Inducing differentiation of human dental pulp cells toward acinar-lineage

TY - JOUR

T1 - Inducing differentiation of human dental pulp cells toward acinar-lineage

AU - Yan, Ming

AU - Kluwe, Lan

AU - Friedrich, Reinhard E

AU - Li, Xiangjun

AU - Ren, Guiyun

AU - Smeets, Ralf

AU - Gosau, Martin

AU - Liu, Xiao

N1 - AJTR Copyright © 2020.

PY - 2020

Y1 - 2020

N2 - OBJECTIVE: To induce acinar-differentiation from human dental pulp cells for potential application in aiding treatment of dry-eye syndromes.METHOD: Human dental pulp cells were co-cultured with human submandibular gland acinar cells using a transwell construction for 2 weeks. The two populations of cells were physically separated while chemical and biochemical components can be exchanged. Fibroblasts were included as a negative control. Expression of amylase, cytokeratin 8 and vimentin were examined by immune-staining. Amylase activity was measured using an AMS Assay Kit.RESULT: Cobblestone-like islands, a feature of acinar cells, appeared in the dental pulp cells which were co-cultured with salivary gland cells for one week and increased in number and size after two weeks. Antibody detected amylase in 30 and 50% of the pulp cells 1 and 2 weeks in the co-culture, respectively. Cytokeratin 8 increased while vimentin decreased. All these changes indicate an acinar-like differentiation of the dental pulp cells. None of these changes were observed in fibroblasts which were also co-cultured with salivary gland cells, indicating that the acinar-like differentiation is specific for the dental pulp cells. Neither of the changes were observed in dental pulp cells when not co-cultured with the salivary gland cells, indicating that induction is specific and essential.CONCLUSIONS: Human dental pulp cells have the potential to differentiate into acinar-like cells which may provide an autologous source for cellular therapy for dry-eye syndromes.

AB - OBJECTIVE: To induce acinar-differentiation from human dental pulp cells for potential application in aiding treatment of dry-eye syndromes.METHOD: Human dental pulp cells were co-cultured with human submandibular gland acinar cells using a transwell construction for 2 weeks. The two populations of cells were physically separated while chemical and biochemical components can be exchanged. Fibroblasts were included as a negative control. Expression of amylase, cytokeratin 8 and vimentin were examined by immune-staining. Amylase activity was measured using an AMS Assay Kit.RESULT: Cobblestone-like islands, a feature of acinar cells, appeared in the dental pulp cells which were co-cultured with salivary gland cells for one week and increased in number and size after two weeks. Antibody detected amylase in 30 and 50% of the pulp cells 1 and 2 weeks in the co-culture, respectively. Cytokeratin 8 increased while vimentin decreased. All these changes indicate an acinar-like differentiation of the dental pulp cells. None of these changes were observed in fibroblasts which were also co-cultured with salivary gland cells, indicating that the acinar-like differentiation is specific for the dental pulp cells. Neither of the changes were observed in dental pulp cells when not co-cultured with the salivary gland cells, indicating that induction is specific and essential.CONCLUSIONS: Human dental pulp cells have the potential to differentiate into acinar-like cells which may provide an autologous source for cellular therapy for dry-eye syndromes.

M3 - SCORING: Journal article

C2 - 33042457

VL - 12

SP - 5781

EP - 5788

JO - AM J TRANSL RES

JF - AM J TRANSL RES

SN - 1943-8141

IS - 9

ER -