Induced and thymus-derived Foxp3⁺ regulatory T cells share a common niche
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Induced and thymus-derived Foxp3⁺ regulatory T cells share a common niche. / Huang, Yi-Ju; Haist, Verena; Baumgärtner, Wolfgang; Föhse, Lisa; Prinz, Immo; Suerbaum, Sebastian; Floess, Stefan; Huehn, Jochen.
In: EUR J IMMUNOL, Vol. 44, No. 2, 02.2014, p. 460-8.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Induced and thymus-derived Foxp3⁺ regulatory T cells share a common niche
AU - Huang, Yi-Ju
AU - Haist, Verena
AU - Baumgärtner, Wolfgang
AU - Föhse, Lisa
AU - Prinz, Immo
AU - Suerbaum, Sebastian
AU - Floess, Stefan
AU - Huehn, Jochen
N1 - © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2014/2
Y1 - 2014/2
N2 - Foxp3⁺ regulatory T (Treg) cells, which play a central role for the maintenance of immune homeostasis and self-tolerance, are known to be both generated in the thymus (thymus-derived, tTreg cells) and in the periphery, where they are converted from conventional CD4⁺ T cells (induced Treg (iTreg) cells). Recent data suggest a division of labor between these two Treg-cell subsets since their combined action was shown to be essential for protection in inflammatory disease models. Here, using the transfer colitis model, we examined whether tTreg cells and iTreg cells fill different niches within the CD4⁺ T-cell compartment. When naive T cells were co-transferred with either pure tTreg cells or with a mixture of tTreg cells and iTreg cells, induction of Foxp3⁺ Treg cells from naive T cells was not hampered by preoccupation of the Treg-cell niche. Using neuropilin-1 (Nrp1) as a surface marker to separate tTreg cells and iTreg cells, we demonstrate that tTreg cells and iTreg cells alone can completely fill the Treg-cell niche and display comparable TCR repertoires. However, when transferred together Nrp1⁺ tTreg cells outcompeted Nrp1⁻ iTreg cells and dominated the Treg-cell compartment. Taken together, our data suggest that tTreg cells and iTreg cells share a common peripheral niche.
AB - Foxp3⁺ regulatory T (Treg) cells, which play a central role for the maintenance of immune homeostasis and self-tolerance, are known to be both generated in the thymus (thymus-derived, tTreg cells) and in the periphery, where they are converted from conventional CD4⁺ T cells (induced Treg (iTreg) cells). Recent data suggest a division of labor between these two Treg-cell subsets since their combined action was shown to be essential for protection in inflammatory disease models. Here, using the transfer colitis model, we examined whether tTreg cells and iTreg cells fill different niches within the CD4⁺ T-cell compartment. When naive T cells were co-transferred with either pure tTreg cells or with a mixture of tTreg cells and iTreg cells, induction of Foxp3⁺ Treg cells from naive T cells was not hampered by preoccupation of the Treg-cell niche. Using neuropilin-1 (Nrp1) as a surface marker to separate tTreg cells and iTreg cells, we demonstrate that tTreg cells and iTreg cells alone can completely fill the Treg-cell niche and display comparable TCR repertoires. However, when transferred together Nrp1⁺ tTreg cells outcompeted Nrp1⁻ iTreg cells and dominated the Treg-cell compartment. Taken together, our data suggest that tTreg cells and iTreg cells share a common peripheral niche.
KW - Animals
KW - CD4-Positive T-Lymphocytes/immunology
KW - Colitis/immunology
KW - Disease Models, Animal
KW - Forkhead Transcription Factors/metabolism
KW - Immune Tolerance/immunology
KW - Inflammation/immunology
KW - Mice
KW - Mice, Inbred BALB C
KW - Mice, Inbred C57BL
KW - Neuropilin-1/immunology
KW - Receptors, Antigen, T-Cell/immunology
KW - T-Lymphocytes, Regulatory/immunology
KW - Thymus Gland/immunology
U2 - 10.1002/eji.201343463
DO - 10.1002/eji.201343463
M3 - SCORING: Journal article
C2 - 24170313
VL - 44
SP - 460
EP - 468
JO - EUR J IMMUNOL
JF - EUR J IMMUNOL
SN - 0014-2980
IS - 2
ER -