Increased interferon alpha expression in circulating plasmacytoid dendritic cells of HIV-1-infected patients.
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Increased interferon alpha expression in circulating plasmacytoid dendritic cells of HIV-1-infected patients. / Lehmann, Clara; Harper, Jill M; Taubert, Dirk; Hartmann, Pia; Fätkenheuer, Gerd; Jung, Norma; van Lunzen, Jan; Stellbrink, Hans-Jürgen; Gallo, Robert C; Romerio, Fabio.
In: JAIDS-J ACQ IMM DEF, Vol. 48, No. 5, 5, 2008, p. 522-530.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Increased interferon alpha expression in circulating plasmacytoid dendritic cells of HIV-1-infected patients.
AU - Lehmann, Clara
AU - Harper, Jill M
AU - Taubert, Dirk
AU - Hartmann, Pia
AU - Fätkenheuer, Gerd
AU - Jung, Norma
AU - van Lunzen, Jan
AU - Stellbrink, Hans-Jürgen
AU - Gallo, Robert C
AU - Romerio, Fabio
PY - 2008
Y1 - 2008
N2 - BACKGROUND: The role of plasmacytoid dendritic cells (pDC) and interferon alpha (IFN alpha) in HIV-1 infection is still unclear. On one hand, HIV-1 disease is associated with a progressive decline of pDC, which displays reduced ability to produce IFN alpha after in vitro challenge. On the other hand, high IFN alpha serum levels in HIV-1-infected individuals have been proposed to promote immune hyper-activation and disease progression. METHODS: We sought to determine whether disappearance of pDC in HIV-1 disease is due to homing in lymphoid tissues. We also studied IFN alpha and myxovirus resistance protein A (MxA) expression in unstimulated pDC and correlated these results with selected clinical and laboratory parameters. RESULTS: We found that pDC decline markedly in peripheral blood of patients progressing to disease but at the same time express much higher levels of IFN alpha and MxA compared to control individuals. On the other hand, we observed steady pDC counts in lymph nodes of HIV-1 patients. The frequency of circulating pDC correlated directly with CD4 cell counts and inversely with viral load. However, we found no correlation between IFN alpha and MxA expression levels, CD4 counts, and viral load. CONCLUSIONS: Circulating pDC decline sharply in the course of HIV-1 disease, but express high levels of IFN alpha, which may represent a hallmark of systemic immune dysfunction.
AB - BACKGROUND: The role of plasmacytoid dendritic cells (pDC) and interferon alpha (IFN alpha) in HIV-1 infection is still unclear. On one hand, HIV-1 disease is associated with a progressive decline of pDC, which displays reduced ability to produce IFN alpha after in vitro challenge. On the other hand, high IFN alpha serum levels in HIV-1-infected individuals have been proposed to promote immune hyper-activation and disease progression. METHODS: We sought to determine whether disappearance of pDC in HIV-1 disease is due to homing in lymphoid tissues. We also studied IFN alpha and myxovirus resistance protein A (MxA) expression in unstimulated pDC and correlated these results with selected clinical and laboratory parameters. RESULTS: We found that pDC decline markedly in peripheral blood of patients progressing to disease but at the same time express much higher levels of IFN alpha and MxA compared to control individuals. On the other hand, we observed steady pDC counts in lymph nodes of HIV-1 patients. The frequency of circulating pDC correlated directly with CD4 cell counts and inversely with viral load. However, we found no correlation between IFN alpha and MxA expression levels, CD4 counts, and viral load. CONCLUSIONS: Circulating pDC decline sharply in the course of HIV-1 disease, but express high levels of IFN alpha, which may represent a hallmark of systemic immune dysfunction.
M3 - SCORING: Zeitschriftenaufsatz
VL - 48
SP - 522
EP - 530
JO - JAIDS-J ACQ IMM DEF
JF - JAIDS-J ACQ IMM DEF
SN - 1525-4135
IS - 5
M1 - 5
ER -
