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Increased HEV seroprevalence in patients with autoimmune hepatitis

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Increased HEV seroprevalence in patients with autoimmune hepatitis. / Pischke, Sven; Gisa, Anett; Suneetha, Pothakamuri Venkata; Wiegand, Steffen Björn; Taubert, Richard; Schlue, Jerome; Wursthorn, Karsten; Bantel, Heike; Raupach, Regina; Bremer, Birgit; Zacher, Behrend Johann; Schmidt, Reinhold Ernst; Manns, Michael Peter; Rifai, Kinan; Witte, Torsten; Wedemeyer, Heiner.

In: PLOS ONE, Vol. 9, No. 1, 01.01.2014, p. e85330.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Pischke, S, Gisa, A, Suneetha, PV, Wiegand, SB, Taubert, R, Schlue, J, Wursthorn, K, Bantel, H, Raupach, R, Bremer, B, Zacher, BJ, Schmidt, RE, Manns, MP, Rifai, K, Witte, T & Wedemeyer, H 2014, 'Increased HEV seroprevalence in patients with autoimmune hepatitis', PLOS ONE, vol. 9, no. 1, pp. e85330. https://doi.org/10.1371/journal.pone.0085330

APA

Pischke, S., Gisa, A., Suneetha, P. V., Wiegand, S. B., Taubert, R., Schlue, J., Wursthorn, K., Bantel, H., Raupach, R., Bremer, B., Zacher, B. J., Schmidt, R. E., Manns, M. P., Rifai, K., Witte, T., & Wedemeyer, H. (2014). Increased HEV seroprevalence in patients with autoimmune hepatitis. PLOS ONE, 9(1), e85330. https://doi.org/10.1371/journal.pone.0085330

Vancouver

Pischke S, Gisa A, Suneetha PV, Wiegand SB, Taubert R, Schlue J et al. Increased HEV seroprevalence in patients with autoimmune hepatitis. PLOS ONE. 2014 Jan 1;9(1):e85330. https://doi.org/10.1371/journal.pone.0085330

Bibtex

@article{bd78cfa90ab44371b2e7cd11f3f20383,
title = "Increased HEV seroprevalence in patients with autoimmune hepatitis",
abstract = "BACKGROUND: Hepatitis E virus (HEV) infection takes a clinically silent, self-limited course in the far majority of cases. Chronic hepatitis E has been reported in some cohorts of immunocompromised individuals. The role of HEV infections in patients with autoimmune hepatitis (AIH) is unknown.METHODS: 969 individuals were tested for anti-HEV antibodies (MP-diagnostics) including 208 patients with AIH, 537 healthy controls, 114 patients with another autoimmune disease, rheumatoid arthritis (RA), and 109 patients with chronic HCV- or HBV-infection (HBV/HCV). Patients with AIH, RA and HBV/HCV were tested for HEV RNA. HEV-specific proliferative T cell responses were investigated using CFSE staining and in vitro stimulation of PBMC with overlapping HEV peptides.RESULTS: HEV-antibodies tested more frequently positive in patients with AIH (n = 16; 7.7%) than in healthy controls (n = 11; 2.0%; p = 0.0002), patients with RA (n = 4; 3.5%; p = 0.13) or patients with HBV/HCV infection (n = 2; 2.8%; p = 0.03). HEV-specific T cell responses could be detected in all anti-HEV-positive AIH patients. One AIH patient receiving immunosuppression with cyclosporin and prednisolone and elevated ALT levels had acute hepatitis E but HEV viremia resolved after reducing immunosuppressive medication. None of the RA or HBV/HCV patients tested HEV RNA positive.CONCLUSIONS: Patients with autoimmune hepatitis but not RA or HBV/HCV patients are more likely to test anti-HEV positive. HEV infection should been ruled out before the diagnosis of AIH is made. Testing for HEV RNA is also recommended in AIH patients not responding to immunosuppressive therapy.",
keywords = "Adolescent, Adult, Aged, Aged, 80 and over, Arthritis, Rheumatoid, Case-Control Studies, Coinfection, Female, Hepatitis Antibodies, Hepatitis B, Hepatitis C, Hepatitis E, Hepatitis E virus, Hepatitis, Autoimmune, Hepatitis, Chronic, Humans, Immunocompromised Host, Male, Middle Aged, RNA, Viral, Seroepidemiologic Studies, T-Lymphocytes",
author = "Sven Pischke and Anett Gisa and Suneetha, {Pothakamuri Venkata} and Wiegand, {Steffen Bj{\"o}rn} and Richard Taubert and Jerome Schlue and Karsten Wursthorn and Heike Bantel and Regina Raupach and Birgit Bremer and Zacher, {Behrend Johann} and Schmidt, {Reinhold Ernst} and Manns, {Michael Peter} and Kinan Rifai and Torsten Witte and Heiner Wedemeyer",
year = "2014",
month = jan,
day = "1",
doi = "10.1371/journal.pone.0085330",
language = "English",
volume = "9",
pages = "e85330",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

RIS

TY - JOUR

T1 - Increased HEV seroprevalence in patients with autoimmune hepatitis

AU - Pischke, Sven

AU - Gisa, Anett

AU - Suneetha, Pothakamuri Venkata

AU - Wiegand, Steffen Björn

AU - Taubert, Richard

AU - Schlue, Jerome

AU - Wursthorn, Karsten

AU - Bantel, Heike

AU - Raupach, Regina

AU - Bremer, Birgit

AU - Zacher, Behrend Johann

AU - Schmidt, Reinhold Ernst

AU - Manns, Michael Peter

AU - Rifai, Kinan

AU - Witte, Torsten

AU - Wedemeyer, Heiner

PY - 2014/1/1

Y1 - 2014/1/1

N2 - BACKGROUND: Hepatitis E virus (HEV) infection takes a clinically silent, self-limited course in the far majority of cases. Chronic hepatitis E has been reported in some cohorts of immunocompromised individuals. The role of HEV infections in patients with autoimmune hepatitis (AIH) is unknown.METHODS: 969 individuals were tested for anti-HEV antibodies (MP-diagnostics) including 208 patients with AIH, 537 healthy controls, 114 patients with another autoimmune disease, rheumatoid arthritis (RA), and 109 patients with chronic HCV- or HBV-infection (HBV/HCV). Patients with AIH, RA and HBV/HCV were tested for HEV RNA. HEV-specific proliferative T cell responses were investigated using CFSE staining and in vitro stimulation of PBMC with overlapping HEV peptides.RESULTS: HEV-antibodies tested more frequently positive in patients with AIH (n = 16; 7.7%) than in healthy controls (n = 11; 2.0%; p = 0.0002), patients with RA (n = 4; 3.5%; p = 0.13) or patients with HBV/HCV infection (n = 2; 2.8%; p = 0.03). HEV-specific T cell responses could be detected in all anti-HEV-positive AIH patients. One AIH patient receiving immunosuppression with cyclosporin and prednisolone and elevated ALT levels had acute hepatitis E but HEV viremia resolved after reducing immunosuppressive medication. None of the RA or HBV/HCV patients tested HEV RNA positive.CONCLUSIONS: Patients with autoimmune hepatitis but not RA or HBV/HCV patients are more likely to test anti-HEV positive. HEV infection should been ruled out before the diagnosis of AIH is made. Testing for HEV RNA is also recommended in AIH patients not responding to immunosuppressive therapy.

AB - BACKGROUND: Hepatitis E virus (HEV) infection takes a clinically silent, self-limited course in the far majority of cases. Chronic hepatitis E has been reported in some cohorts of immunocompromised individuals. The role of HEV infections in patients with autoimmune hepatitis (AIH) is unknown.METHODS: 969 individuals were tested for anti-HEV antibodies (MP-diagnostics) including 208 patients with AIH, 537 healthy controls, 114 patients with another autoimmune disease, rheumatoid arthritis (RA), and 109 patients with chronic HCV- or HBV-infection (HBV/HCV). Patients with AIH, RA and HBV/HCV were tested for HEV RNA. HEV-specific proliferative T cell responses were investigated using CFSE staining and in vitro stimulation of PBMC with overlapping HEV peptides.RESULTS: HEV-antibodies tested more frequently positive in patients with AIH (n = 16; 7.7%) than in healthy controls (n = 11; 2.0%; p = 0.0002), patients with RA (n = 4; 3.5%; p = 0.13) or patients with HBV/HCV infection (n = 2; 2.8%; p = 0.03). HEV-specific T cell responses could be detected in all anti-HEV-positive AIH patients. One AIH patient receiving immunosuppression with cyclosporin and prednisolone and elevated ALT levels had acute hepatitis E but HEV viremia resolved after reducing immunosuppressive medication. None of the RA or HBV/HCV patients tested HEV RNA positive.CONCLUSIONS: Patients with autoimmune hepatitis but not RA or HBV/HCV patients are more likely to test anti-HEV positive. HEV infection should been ruled out before the diagnosis of AIH is made. Testing for HEV RNA is also recommended in AIH patients not responding to immunosuppressive therapy.

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Arthritis, Rheumatoid

KW - Case-Control Studies

KW - Coinfection

KW - Female

KW - Hepatitis Antibodies

KW - Hepatitis B

KW - Hepatitis C

KW - Hepatitis E

KW - Hepatitis E virus

KW - Hepatitis, Autoimmune

KW - Hepatitis, Chronic

KW - Humans

KW - Immunocompromised Host

KW - Male

KW - Middle Aged

KW - RNA, Viral

KW - Seroepidemiologic Studies

KW - T-Lymphocytes

U2 - 10.1371/journal.pone.0085330

DO - 10.1371/journal.pone.0085330

M3 - SCORING: Journal article

C2 - 24465537

VL - 9

SP - e85330

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 1

ER -