Increased bone density and resistance to ovariectomy-induced bone loss in FoxP3-transgenic mice based on impaired osteoclast differentiation

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Increased bone density and resistance to ovariectomy-induced bone loss in FoxP3-transgenic mice based on impaired osteoclast differentiation. / Zaiss, Mario M; Sarter, Kerstin; Hess, Andreas; Engelke, Klaus; Böhm, Christina; Nimmerjahn, Falk; Voll, Reinhard; Schett, Georg; David, Jean-Pierre.

In: ARTHRITIS RHEUMATOL, Vol. 62, No. 8, 01.08.2010, p. 2328-38.

Research output: SCORING: Contribution to journalSCORING: Journal articleResearchpeer-review

Harvard

Zaiss, MM, Sarter, K, Hess, A, Engelke, K, Böhm, C, Nimmerjahn, F, Voll, R, Schett, G & David, J-P 2010, 'Increased bone density and resistance to ovariectomy-induced bone loss in FoxP3-transgenic mice based on impaired osteoclast differentiation', ARTHRITIS RHEUMATOL, vol. 62, no. 8, pp. 2328-38. https://doi.org/10.1002/art.27535

APA

Zaiss, M. M., Sarter, K., Hess, A., Engelke, K., Böhm, C., Nimmerjahn, F., Voll, R., Schett, G., & David, J-P. (2010). Increased bone density and resistance to ovariectomy-induced bone loss in FoxP3-transgenic mice based on impaired osteoclast differentiation. ARTHRITIS RHEUMATOL, 62(8), 2328-38. https://doi.org/10.1002/art.27535

Vancouver

Bibtex

@article{1a1b365f6bb6431ea6a07dfec1f5c098,
title = "Increased bone density and resistance to ovariectomy-induced bone loss in FoxP3-transgenic mice based on impaired osteoclast differentiation",
abstract = "OBJECTIVE: Immune activation triggers bone loss. Activated T cells are the cellular link between immune activation and bone destruction. The aim of this study was to determine whether immune regulatory mechanisms, such as naturally occurring Treg cells, also extend their protective effects to bone homeostasis in vivo.METHODS: Bone parameters in FoxP3-transgenic (Tg) mice were compared with those in their wild-type (WT) littermate controls. Ovariectomy was performed in FoxP3-Tg mice as a model of postmenopausal osteoporosis, and the bone parameters were analyzed. The bones of RAG-1(-/-) mice were analyzed following the adoptive transfer of isolated CD4+CD25+ T cells. CD4+CD25+ T cells and CD4+ T cells isolated from FoxP3-Tg mice and WT mice were cocultured with monocytes to determine their ability to suppress osteoclastogenesis in vitro.RESULTS: FoxP3-Tg mice developed higher bone mass and were protected from ovariectomy-induced bone loss. The increase in bone mass was found to be the result of impaired osteoclast differentiation and bone resorption in vivo. Bone formation was not affected. Adoptive transfer of CD4+CD25+ T cells into T cell-deficient RAG-1(-/-) mice also increased the bone mass, indicating that Treg cells directly affect bone homeostasis without the need to engage other T cell lineages.CONCLUSION: These data demonstrate that Treg cells can control bone resorption in vivo and can preserve bone mass during physiologic and pathologic bone remodeling.",
keywords = "Animals, Bone Density, Bone Resorption, Bone and Bones, CD4-Positive T-Lymphocytes, Cell Differentiation, Cells, Cultured, Flow Cytometry, Forkhead Transcription Factors, Immunohistochemistry, Mice, Mice, Knockout, Osteoclasts, Ovariectomy, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocyte Subsets",
author = "Zaiss, {Mario M} and Kerstin Sarter and Andreas Hess and Klaus Engelke and Christina B{\"o}hm and Falk Nimmerjahn and Reinhard Voll and Georg Schett and Jean-Pierre David",
year = "2010",
month = aug,
day = "1",
doi = "10.1002/art.27535",
language = "English",
volume = "62",
pages = "2328--38",
journal = "ARTHRITIS RHEUMATOL",
issn = "2326-5191",
publisher = "John Wiley and Sons Ltd",
number = "8",

}

RIS

TY - JOUR

T1 - Increased bone density and resistance to ovariectomy-induced bone loss in FoxP3-transgenic mice based on impaired osteoclast differentiation

AU - Zaiss, Mario M

AU - Sarter, Kerstin

AU - Hess, Andreas

AU - Engelke, Klaus

AU - Böhm, Christina

AU - Nimmerjahn, Falk

AU - Voll, Reinhard

AU - Schett, Georg

AU - David, Jean-Pierre

PY - 2010/8/1

Y1 - 2010/8/1

N2 - OBJECTIVE: Immune activation triggers bone loss. Activated T cells are the cellular link between immune activation and bone destruction. The aim of this study was to determine whether immune regulatory mechanisms, such as naturally occurring Treg cells, also extend their protective effects to bone homeostasis in vivo.METHODS: Bone parameters in FoxP3-transgenic (Tg) mice were compared with those in their wild-type (WT) littermate controls. Ovariectomy was performed in FoxP3-Tg mice as a model of postmenopausal osteoporosis, and the bone parameters were analyzed. The bones of RAG-1(-/-) mice were analyzed following the adoptive transfer of isolated CD4+CD25+ T cells. CD4+CD25+ T cells and CD4+ T cells isolated from FoxP3-Tg mice and WT mice were cocultured with monocytes to determine their ability to suppress osteoclastogenesis in vitro.RESULTS: FoxP3-Tg mice developed higher bone mass and were protected from ovariectomy-induced bone loss. The increase in bone mass was found to be the result of impaired osteoclast differentiation and bone resorption in vivo. Bone formation was not affected. Adoptive transfer of CD4+CD25+ T cells into T cell-deficient RAG-1(-/-) mice also increased the bone mass, indicating that Treg cells directly affect bone homeostasis without the need to engage other T cell lineages.CONCLUSION: These data demonstrate that Treg cells can control bone resorption in vivo and can preserve bone mass during physiologic and pathologic bone remodeling.

AB - OBJECTIVE: Immune activation triggers bone loss. Activated T cells are the cellular link between immune activation and bone destruction. The aim of this study was to determine whether immune regulatory mechanisms, such as naturally occurring Treg cells, also extend their protective effects to bone homeostasis in vivo.METHODS: Bone parameters in FoxP3-transgenic (Tg) mice were compared with those in their wild-type (WT) littermate controls. Ovariectomy was performed in FoxP3-Tg mice as a model of postmenopausal osteoporosis, and the bone parameters were analyzed. The bones of RAG-1(-/-) mice were analyzed following the adoptive transfer of isolated CD4+CD25+ T cells. CD4+CD25+ T cells and CD4+ T cells isolated from FoxP3-Tg mice and WT mice were cocultured with monocytes to determine their ability to suppress osteoclastogenesis in vitro.RESULTS: FoxP3-Tg mice developed higher bone mass and were protected from ovariectomy-induced bone loss. The increase in bone mass was found to be the result of impaired osteoclast differentiation and bone resorption in vivo. Bone formation was not affected. Adoptive transfer of CD4+CD25+ T cells into T cell-deficient RAG-1(-/-) mice also increased the bone mass, indicating that Treg cells directly affect bone homeostasis without the need to engage other T cell lineages.CONCLUSION: These data demonstrate that Treg cells can control bone resorption in vivo and can preserve bone mass during physiologic and pathologic bone remodeling.

KW - Animals

KW - Bone Density

KW - Bone Resorption

KW - Bone and Bones

KW - CD4-Positive T-Lymphocytes

KW - Cell Differentiation

KW - Cells, Cultured

KW - Flow Cytometry

KW - Forkhead Transcription Factors

KW - Immunohistochemistry

KW - Mice

KW - Mice, Knockout

KW - Osteoclasts

KW - Ovariectomy

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - T-Lymphocyte Subsets

U2 - 10.1002/art.27535

DO - 10.1002/art.27535

M3 - SCORING: Journal article

C2 - 20506516

VL - 62

SP - 2328

EP - 2338

JO - ARTHRITIS RHEUMATOL

JF - ARTHRITIS RHEUMATOL

SN - 2326-5191

IS - 8

ER -