Increased alpha2,6-sialylation of surface proteins on tolerogenic, immature dendritic cells and regulatory T cells

Jutta Jenner; Gunter Kerst; Rupert Handgretinger; Ingo Müller

doi:10.1016/j.exphem.2006.04.016

Increased alpha2,6-sialylation of surface proteins on tolerogenic, immature dendritic cells and regulatory T cells

Standard

Increased alpha2,6-sialylation of surface proteins on tolerogenic, immature dendritic cells and regulatory T cells. / Jenner, Jutta; Kerst, Gunter; Handgretinger, Rupert; Müller, Ingo.

In: Experimental hematology, Vol. 34, No. 9, 09.2006, p. 1212-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Jenner, J, Kerst, G, Handgretinger, R & Müller, I 2006, 'Increased alpha2,6-sialylation of surface proteins on tolerogenic, immature dendritic cells and regulatory T cells', Experimental hematology, vol. 34, no. 9, pp. 1212-8. https://doi.org/10.1016/j.exphem.2006.04.016

APA

Jenner, J., Kerst, G., Handgretinger, R., & Müller, I. (2006). Increased alpha2,6-sialylation of surface proteins on tolerogenic, immature dendritic cells and regulatory T cells. Experimental hematology, 34(9), 1212-8. https://doi.org/10.1016/j.exphem.2006.04.016

Vancouver

Jenner J, Kerst G, Handgretinger R, Müller I. Increased alpha2,6-sialylation of surface proteins on tolerogenic, immature dendritic cells and regulatory T cells. Experimental hematology. 2006 Sep;34(9):1212-8. https://doi.org/10.1016/j.exphem.2006.04.016

Bibtex

@article{cbd9830fa3b24acfbd1298387c5382f7,

title = "Increased alpha2,6-sialylation of surface proteins on tolerogenic, immature dendritic cells and regulatory T cells",

abstract = "OBJECTIVE: Surface protein glycosylation of lymphocytes plays a key role in development, maturation, and immune regulation. Sialic acid most often is the terminal carbohydrate in these posttranslational modifications. Receptors for sialic acids are expressed on lymphocytes and can generate an inhibitory signal. This study compared the sialic acid expression pattern of tolerogenic cells and effector cells.METHODS: Gene expression profiles of immature and mature monocyte-derived dendritic cells were compared using cDNA array technology. We analyzed the cell-surface protein sialylation of dendritic cells and different T cell subpopulations by flow cytometry using plant lectins.RESULTS: Monocyte-derived dendritic cells showed a separation according to alpha2,6-linked sialic acid density. Tolerogenic, immature DC showed a higher alpha2,6-linked sialic acid, which was drastically downregulated after maturation of DC with proinflammatory cytokines. This differential expression of alpha2,6-linked sialic acid was reflected by transcriptional regulation of specific glycosyl transferases during DC maturation shown by cDNA array analysis. Furthermore, CD4(+) T cells significantly upregulated alpha2,6-linked sialic acid density, whereas alpha2,3-linked sialic acid density remained largely unchanged after stimulation. Isolated CD4(+)CD25(+) T cells showed a population with high density of alpha2,6-linked sialic acid and a population with low expression. The density of this particular carbohydrate was further increased during culture conditions expanding inhibitory T cells.CONCLUSION: Surface proteins on tolerogenic, immature dendritic cells and regulatory T cells are highly alpha2,6-sialylated, suggesting a glycan motif of tolerogenic cells which might serve as ligand for inhibitory siglecs on the surface of effector cells.",

keywords = "Carbohydrate Sequence, Cell Differentiation, Cells, Cultured, Dendritic Cells, Down-Regulation, Gene Expression Profiling, Humans, Immune Tolerance, Membrane Proteins, N-Acetylneuraminic Acid, Protein Processing, Post-Translational, Sialyltransferases, Signal Transduction, T-Lymphocytes, Regulatory, Up-Regulation",

author = "Jutta Jenner and Gunter Kerst and Rupert Handgretinger and Ingo M{\"u}ller",

year = "2006",

month = sep,

doi = "10.1016/j.exphem.2006.04.016",

language = "English",

volume = "34",

pages = "1212--8",

journal = "EXP HEMATOL",

issn = "0301-472X",

publisher = "Elsevier Inc.",

number = "9",

}

RIS

TY - JOUR

T1 - Increased alpha2,6-sialylation of surface proteins on tolerogenic, immature dendritic cells and regulatory T cells

AU - Jenner, Jutta

AU - Kerst, Gunter

AU - Handgretinger, Rupert

AU - Müller, Ingo

PY - 2006/9

Y1 - 2006/9

N2 - OBJECTIVE: Surface protein glycosylation of lymphocytes plays a key role in development, maturation, and immune regulation. Sialic acid most often is the terminal carbohydrate in these posttranslational modifications. Receptors for sialic acids are expressed on lymphocytes and can generate an inhibitory signal. This study compared the sialic acid expression pattern of tolerogenic cells and effector cells.METHODS: Gene expression profiles of immature and mature monocyte-derived dendritic cells were compared using cDNA array technology. We analyzed the cell-surface protein sialylation of dendritic cells and different T cell subpopulations by flow cytometry using plant lectins.RESULTS: Monocyte-derived dendritic cells showed a separation according to alpha2,6-linked sialic acid density. Tolerogenic, immature DC showed a higher alpha2,6-linked sialic acid, which was drastically downregulated after maturation of DC with proinflammatory cytokines. This differential expression of alpha2,6-linked sialic acid was reflected by transcriptional regulation of specific glycosyl transferases during DC maturation shown by cDNA array analysis. Furthermore, CD4(+) T cells significantly upregulated alpha2,6-linked sialic acid density, whereas alpha2,3-linked sialic acid density remained largely unchanged after stimulation. Isolated CD4(+)CD25(+) T cells showed a population with high density of alpha2,6-linked sialic acid and a population with low expression. The density of this particular carbohydrate was further increased during culture conditions expanding inhibitory T cells.CONCLUSION: Surface proteins on tolerogenic, immature dendritic cells and regulatory T cells are highly alpha2,6-sialylated, suggesting a glycan motif of tolerogenic cells which might serve as ligand for inhibitory siglecs on the surface of effector cells.

AB - OBJECTIVE: Surface protein glycosylation of lymphocytes plays a key role in development, maturation, and immune regulation. Sialic acid most often is the terminal carbohydrate in these posttranslational modifications. Receptors for sialic acids are expressed on lymphocytes and can generate an inhibitory signal. This study compared the sialic acid expression pattern of tolerogenic cells and effector cells.METHODS: Gene expression profiles of immature and mature monocyte-derived dendritic cells were compared using cDNA array technology. We analyzed the cell-surface protein sialylation of dendritic cells and different T cell subpopulations by flow cytometry using plant lectins.RESULTS: Monocyte-derived dendritic cells showed a separation according to alpha2,6-linked sialic acid density. Tolerogenic, immature DC showed a higher alpha2,6-linked sialic acid, which was drastically downregulated after maturation of DC with proinflammatory cytokines. This differential expression of alpha2,6-linked sialic acid was reflected by transcriptional regulation of specific glycosyl transferases during DC maturation shown by cDNA array analysis. Furthermore, CD4(+) T cells significantly upregulated alpha2,6-linked sialic acid density, whereas alpha2,3-linked sialic acid density remained largely unchanged after stimulation. Isolated CD4(+)CD25(+) T cells showed a population with high density of alpha2,6-linked sialic acid and a population with low expression. The density of this particular carbohydrate was further increased during culture conditions expanding inhibitory T cells.CONCLUSION: Surface proteins on tolerogenic, immature dendritic cells and regulatory T cells are highly alpha2,6-sialylated, suggesting a glycan motif of tolerogenic cells which might serve as ligand for inhibitory siglecs on the surface of effector cells.

KW - Carbohydrate Sequence

KW - Cell Differentiation

KW - Cells, Cultured

KW - Dendritic Cells

KW - Down-Regulation

KW - Gene Expression Profiling

KW - Humans

KW - Immune Tolerance

KW - Membrane Proteins

KW - N-Acetylneuraminic Acid

KW - Protein Processing, Post-Translational

KW - Sialyltransferases

KW - Signal Transduction

KW - T-Lymphocytes, Regulatory

KW - Up-Regulation

U2 - 10.1016/j.exphem.2006.04.016

DO - 10.1016/j.exphem.2006.04.016

M3 - SCORING: Journal article

C2 - 16939814

VL - 34

SP - 1212

EP - 1218

JO - EXP HEMATOL

JF - EXP HEMATOL

SN - 0301-472X

IS - 9

ER -