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In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in cardiac disease

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In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in cardiac disease. / Sprenger, Julia U; Perera, Ruwan K; Steinbrecher, Julia H; Lehnart, Stephan E; Maier, Lars S; Hasenfuss, Gerd; Nikolaev, Viacheslav O.

In: NAT COMMUN, Vol. 6, 04.2015, p. Art. 6965.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Sprenger, JU, Perera, RK, Steinbrecher, JH, Lehnart, SE, Maier, LS, Hasenfuss, G & Nikolaev, VO 2015, 'In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in cardiac disease', NAT COMMUN, vol. 6, pp. Art. 6965. https://doi.org/10.1038/ncomms7965

APA

Sprenger, J. U., Perera, R. K., Steinbrecher, J. H., Lehnart, S. E., Maier, L. S., Hasenfuss, G., & Nikolaev, V. O. (2015). In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in cardiac disease. NAT COMMUN, 6, Art. 6965. https://doi.org/10.1038/ncomms7965

Vancouver

Sprenger JU, Perera RK, Steinbrecher JH, Lehnart SE, Maier LS, Hasenfuss G et al. In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in cardiac disease. NAT COMMUN. 2015 Apr;6:Art. 6965. https://doi.org/10.1038/ncomms7965

Bibtex

@article{593ba5213be54729a3d2397d007578a9,
title = "In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in cardiac disease",
abstract = "3',5'-cyclic adenosine monophosphate (cAMP) is an ubiquitous second messenger that regulates physiological functions by acting in distinct subcellular microdomains. Although several targeted cAMP biosensors are developed and used in single cells, it is unclear whether such biosensors can be successfully applied in vivo, especially in the context of disease. Here, we describe a transgenic mouse model expressing a targeted cAMP sensor and analyse microdomain-specific second messenger dynamics in the vicinity of the sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA). We demonstrate the biocompatibility of this targeted sensor and its potential for real-time monitoring of compartmentalized cAMP signalling in adult cardiomyocytes isolated from a healthy mouse heart and from an in vivo cardiac disease model. In particular, we uncover the existence of a phosphodiesterase-dependent receptor-microdomain communication, which is affected in hypertrophy, resulting in reduced β-adrenergic receptor-cAMP signalling to SERCA.",
author = "Sprenger, {Julia U} and Perera, {Ruwan K} and Steinbrecher, {Julia H} and Lehnart, {Stephan E} and Maier, {Lars S} and Gerd Hasenfuss and Nikolaev, {Viacheslav O}",
year = "2015",
month = apr,
doi = "10.1038/ncomms7965",
language = "English",
volume = "6",
pages = "Art. 6965",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in cardiac disease

AU - Sprenger, Julia U

AU - Perera, Ruwan K

AU - Steinbrecher, Julia H

AU - Lehnart, Stephan E

AU - Maier, Lars S

AU - Hasenfuss, Gerd

AU - Nikolaev, Viacheslav O

PY - 2015/4

Y1 - 2015/4

N2 - 3',5'-cyclic adenosine monophosphate (cAMP) is an ubiquitous second messenger that regulates physiological functions by acting in distinct subcellular microdomains. Although several targeted cAMP biosensors are developed and used in single cells, it is unclear whether such biosensors can be successfully applied in vivo, especially in the context of disease. Here, we describe a transgenic mouse model expressing a targeted cAMP sensor and analyse microdomain-specific second messenger dynamics in the vicinity of the sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA). We demonstrate the biocompatibility of this targeted sensor and its potential for real-time monitoring of compartmentalized cAMP signalling in adult cardiomyocytes isolated from a healthy mouse heart and from an in vivo cardiac disease model. In particular, we uncover the existence of a phosphodiesterase-dependent receptor-microdomain communication, which is affected in hypertrophy, resulting in reduced β-adrenergic receptor-cAMP signalling to SERCA.

AB - 3',5'-cyclic adenosine monophosphate (cAMP) is an ubiquitous second messenger that regulates physiological functions by acting in distinct subcellular microdomains. Although several targeted cAMP biosensors are developed and used in single cells, it is unclear whether such biosensors can be successfully applied in vivo, especially in the context of disease. Here, we describe a transgenic mouse model expressing a targeted cAMP sensor and analyse microdomain-specific second messenger dynamics in the vicinity of the sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA). We demonstrate the biocompatibility of this targeted sensor and its potential for real-time monitoring of compartmentalized cAMP signalling in adult cardiomyocytes isolated from a healthy mouse heart and from an in vivo cardiac disease model. In particular, we uncover the existence of a phosphodiesterase-dependent receptor-microdomain communication, which is affected in hypertrophy, resulting in reduced β-adrenergic receptor-cAMP signalling to SERCA.

U2 - 10.1038/ncomms7965

DO - 10.1038/ncomms7965

M3 - SCORING: Journal article

C2 - 25917898

VL - 6

SP - Art. 6965

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

ER -