In vivo magnetic resonance imaging of iron oxide-labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury: detection and monitoring at 3T.
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In vivo magnetic resonance imaging of iron oxide-labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury: detection and monitoring at 3T. / Ittrich, Harald; Lange, Claudia; Tögel, Florian; Zander, Axel R.; Dahnke, Hannes; Westenfelder, Christof; Adam, Gerhard; Nolte-Ernsting, Claus.
In: J MAGN RESON IMAGING, Vol. 25, No. 6, 6, 01.06.2007, p. 1179-1191.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - In vivo magnetic resonance imaging of iron oxide-labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury: detection and monitoring at 3T.
AU - Ittrich, Harald
AU - Lange, Claudia
AU - Tögel, Florian
AU - Zander, Axel R.
AU - Dahnke, Hannes
AU - Westenfelder, Christof
AU - Adam, Gerhard
AU - Nolte-Ernsting, Claus
N1 - (c) 2007 Wiley-Liss, Inc.
PY - 2007/6/1
Y1 - 2007/6/1
N2 - PURPOSE: To evaluate MRI for a qualitative and quantitative in vivo tracking of intraaortal injected iron oxide-labeled mesenchymal stem cells (MSC) into rats with acute kidney injury (AKI). MATERIALS AND METHODS: In vitro MRI and R2* measurement of nonlabeled and superparamagnetic iron oxide (SPIO)-labeled MSC (MSC(SPIO)) was performed in correlation to cellular iron content and cytological examination (Prussian blue, electron microscopy). In vivo MRI and R2* evaluation were performed before and after ischemic/reperfusion AKI (N = 14) and intraaortal injection of 1.5 x 10(6) MSC(SPIO) (N = 7), fetal calf serum (FCS) (medium, N = 6), and SPIO alone (N = 1) up to 14 days using a clinical 3T scanner. Signal to noise ratios (SNR), R2* of kidneys, liver, spleen, and bone marrow, renal function (creatinine [CREA], blood urea nitrogen [BUN]), and kidney volume were measured and tested for statistical significance (Student's t-test, P <0.05) in comparison histology (hematoxylin and eosin [H;E], Prussian blue, periodic acid-Schiff [PAS], CD68). RESULTS: In vitro, MSC(SPIO) showed a reduction of SNR and T2* with R2* approximately number of MSC(SPIO) (R2 = 0.98). In vivo MSC(SPIO) administration resulted in a SNR decrease (35 +/- 15%) and R2* increase (101 +/- 18.3%) in renal cortex caused by MSC(SPIO) accumulation in contrast to control animals (P <0.01). Liver, spleen, and bone marrow (MSC(SPIO)) showed a delayed SNR decline/R2* increase (P <0.05) resulting from MSC(SPIO) migration. The increase of kidney volume and the decrease in renal function (P <0.05) was reduced in MSC-treated animals. CONCLUSION: Qualitative and quantitative in vivo cell-tracking and monitoring of organ distribution of intraaortal injected MSC(SPIO) in AKI is feasible in MRI at 3T.
AB - PURPOSE: To evaluate MRI for a qualitative and quantitative in vivo tracking of intraaortal injected iron oxide-labeled mesenchymal stem cells (MSC) into rats with acute kidney injury (AKI). MATERIALS AND METHODS: In vitro MRI and R2* measurement of nonlabeled and superparamagnetic iron oxide (SPIO)-labeled MSC (MSC(SPIO)) was performed in correlation to cellular iron content and cytological examination (Prussian blue, electron microscopy). In vivo MRI and R2* evaluation were performed before and after ischemic/reperfusion AKI (N = 14) and intraaortal injection of 1.5 x 10(6) MSC(SPIO) (N = 7), fetal calf serum (FCS) (medium, N = 6), and SPIO alone (N = 1) up to 14 days using a clinical 3T scanner. Signal to noise ratios (SNR), R2* of kidneys, liver, spleen, and bone marrow, renal function (creatinine [CREA], blood urea nitrogen [BUN]), and kidney volume were measured and tested for statistical significance (Student's t-test, P <0.05) in comparison histology (hematoxylin and eosin [H;E], Prussian blue, periodic acid-Schiff [PAS], CD68). RESULTS: In vitro, MSC(SPIO) showed a reduction of SNR and T2* with R2* approximately number of MSC(SPIO) (R2 = 0.98). In vivo MSC(SPIO) administration resulted in a SNR decrease (35 +/- 15%) and R2* increase (101 +/- 18.3%) in renal cortex caused by MSC(SPIO) accumulation in contrast to control animals (P <0.01). Liver, spleen, and bone marrow (MSC(SPIO)) showed a delayed SNR decline/R2* increase (P <0.05) resulting from MSC(SPIO) migration. The increase of kidney volume and the decrease in renal function (P <0.05) was reduced in MSC-treated animals. CONCLUSION: Qualitative and quantitative in vivo cell-tracking and monitoring of organ distribution of intraaortal injected MSC(SPIO) in AKI is feasible in MRI at 3T.
KW - Animals
KW - Contrast Media
KW - Dextrans
KW - Feasibility Studies
KW - Ferrosoferric Oxide
KW - Image Processing, Computer-Assisted
KW - Injections, Intra-Arterial
KW - Iron
KW - Ischemia
KW - Kidney
KW - Magnetic Resonance Imaging
KW - Magnetite Nanoparticles
KW - Mesenchymal Stem Cell Transplantation
KW - Oxides
KW - Rats
KW - Rats, Sprague-Dawley
KW - Staining and Labeling
U2 - 10.1002/jmri.20925
DO - 10.1002/jmri.20925
M3 - SCORING: Journal article
C2 - 17520738
VL - 25
SP - 1179
EP - 1191
JO - J MAGN RESON IMAGING
JF - J MAGN RESON IMAGING
SN - 1053-1807
IS - 6
M1 - 6
ER -
