In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase

P Greimel; Sabrina Jabs; Stephan Storch; Slim Cherif; Koichi Honke; Thomas Braulke; Joachim Thiem

doi:10.1016/j.carres.2006.02.006

In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase

P Greimel
Sabrina Jabs (Shared first author)
Stephan Storch
Slim Cherif
Koichi Honke
Thomas Braulke
Joachim Thiem

Related Research units

Department of Pediatrics

Abstract

Membrane-bound beta-Gal-3'-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm-Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of beta-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.

Bibliographical data

Original language	English
ISSN	0008-6215
DOIs	https://doi.org/10.1016/j.carres.2006.02.006
Publication status	Published - 22.05.2006

PubMed	16516177