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Improved particle-packed HPLC/MS microchips for proteomic analysis

Standard

Improved particle-packed HPLC/MS microchips for proteomic analysis. / Trusch, Maria; Ehlert, Steffen; Bertsch, Andreas; Kohlbacher, Oliver; Hildebrand, Diana; Schlüter, Hartmut; Tallarek, Ulrich.

In: J SEP SCI, Vol. 33, No. 21, 11.2010, p. 3283-91.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Trusch, M, Ehlert, S, Bertsch, A, Kohlbacher, O, Hildebrand, D, Schlüter, H & Tallarek, U 2010, 'Improved particle-packed HPLC/MS microchips for proteomic analysis', J SEP SCI, vol. 33, no. 21, pp. 3283-91. https://doi.org/10.1002/jssc.201000474

APA

Trusch, M., Ehlert, S., Bertsch, A., Kohlbacher, O., Hildebrand, D., Schlüter, H., & Tallarek, U. (2010). Improved particle-packed HPLC/MS microchips for proteomic analysis. J SEP SCI, 33(21), 3283-91. https://doi.org/10.1002/jssc.201000474

Vancouver

Trusch M, Ehlert S, Bertsch A, Kohlbacher O, Hildebrand D, Schlüter H et al. Improved particle-packed HPLC/MS microchips for proteomic analysis. J SEP SCI. 2010 Nov;33(21):3283-91. https://doi.org/10.1002/jssc.201000474

Bibtex

@article{5f643e76a40c45038d66d6389e93bb0c,
title = "Improved particle-packed HPLC/MS microchips for proteomic analysis",
abstract = "The influence of packing process parameters (packing pressure, application of ultrasound) and the stationary phase particle size (3.5 and 5 μm) on the chromatographic performance of HPLC/MS chips was systematically investigated for proteomic samples. First, reproducibility and detection limits of the separation were evaluated with a low-complexity sample of tryptic BSA peptides. The influence of adsorbent packing quality on protein identification was then tested with a typical proteomics sample of high complexity, a human plasma protein fraction (Cohn fraction IV-4). All HPLC/MS chips provided highly reproducible separations of these proteomic samples, but improved packing conditions and smaller particle sizes resulted in chromatograms with narrower peaks and correspondingly higher signal intensities. Improved separation performance increased the peak capacity, the number of identified peptides, and thus the sequence coverage in the proteomic samples, particularly for low sample amounts.",
keywords = "Blood Proteins, Chromatography, High Pressure Liquid, Humans, Lab-On-A-Chip Devices, Microchip Analytical Procedures, Proteomics, Tandem Mass Spectrometry, Trypsin, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't",
author = "Maria Trusch and Steffen Ehlert and Andreas Bertsch and Oliver Kohlbacher and Diana Hildebrand and Hartmut Schl{\"u}ter and Ulrich Tallarek",
year = "2010",
month = nov,
doi = "10.1002/jssc.201000474",
language = "English",
volume = "33",
pages = "3283--91",
journal = "J SEP SCI",
issn = "1615-9306",
publisher = "Wiley-VCH Verlag GmbH",
number = "21",

}

RIS

TY - JOUR

T1 - Improved particle-packed HPLC/MS microchips for proteomic analysis

AU - Trusch, Maria

AU - Ehlert, Steffen

AU - Bertsch, Andreas

AU - Kohlbacher, Oliver

AU - Hildebrand, Diana

AU - Schlüter, Hartmut

AU - Tallarek, Ulrich

PY - 2010/11

Y1 - 2010/11

N2 - The influence of packing process parameters (packing pressure, application of ultrasound) and the stationary phase particle size (3.5 and 5 μm) on the chromatographic performance of HPLC/MS chips was systematically investigated for proteomic samples. First, reproducibility and detection limits of the separation were evaluated with a low-complexity sample of tryptic BSA peptides. The influence of adsorbent packing quality on protein identification was then tested with a typical proteomics sample of high complexity, a human plasma protein fraction (Cohn fraction IV-4). All HPLC/MS chips provided highly reproducible separations of these proteomic samples, but improved packing conditions and smaller particle sizes resulted in chromatograms with narrower peaks and correspondingly higher signal intensities. Improved separation performance increased the peak capacity, the number of identified peptides, and thus the sequence coverage in the proteomic samples, particularly for low sample amounts.

AB - The influence of packing process parameters (packing pressure, application of ultrasound) and the stationary phase particle size (3.5 and 5 μm) on the chromatographic performance of HPLC/MS chips was systematically investigated for proteomic samples. First, reproducibility and detection limits of the separation were evaluated with a low-complexity sample of tryptic BSA peptides. The influence of adsorbent packing quality on protein identification was then tested with a typical proteomics sample of high complexity, a human plasma protein fraction (Cohn fraction IV-4). All HPLC/MS chips provided highly reproducible separations of these proteomic samples, but improved packing conditions and smaller particle sizes resulted in chromatograms with narrower peaks and correspondingly higher signal intensities. Improved separation performance increased the peak capacity, the number of identified peptides, and thus the sequence coverage in the proteomic samples, particularly for low sample amounts.

KW - Blood Proteins

KW - Chromatography, High Pressure Liquid

KW - Humans

KW - Lab-On-A-Chip Devices

KW - Microchip Analytical Procedures

KW - Proteomics

KW - Tandem Mass Spectrometry

KW - Trypsin

KW - Evaluation Studies

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/jssc.201000474

DO - 10.1002/jssc.201000474

M3 - SCORING: Journal article

C2 - 21049518

VL - 33

SP - 3283

EP - 3291

JO - J SEP SCI

JF - J SEP SCI

SN - 1615-9306

IS - 21

ER -