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Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model

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Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model. / Castro, Liesa; Geertz, Birgit; Reinsch, Marina; Aksehirlioglu, Bülent; Hansen, Arne; Eschenhagen, Thomas; Reichenspurner, Hermann; Weinberger, Florian; Pecha, Simon.

In: JOVE-J VIS EXP, No. 145, 18.03.2019.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Castro, L, Geertz, B, Reinsch, M, Aksehirlioglu, B, Hansen, A, Eschenhagen, T, Reichenspurner, H, Weinberger, F & Pecha, S 2019, 'Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model', JOVE-J VIS EXP, no. 145. https://doi.org/10.3791/58810

APA

Castro, L., Geertz, B., Reinsch, M., Aksehirlioglu, B., Hansen, A., Eschenhagen, T., Reichenspurner, H., Weinberger, F., & Pecha, S. (2019). Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model. JOVE-J VIS EXP, (145). https://doi.org/10.3791/58810

Vancouver

Castro L, Geertz B, Reinsch M, Aksehirlioglu B, Hansen A, Eschenhagen T et al. Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model. JOVE-J VIS EXP. 2019 Mar 18;(145). https://doi.org/10.3791/58810

Bibtex

@article{1047222a3062477c9cd3973d5933753e,
title = "Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model",
abstract = "Due to the limited regeneration capacity of the heart in adult mammals, myocardial infarction results in an irreversible loss of cardiomyocytes. This loss of relevant amounts of heart muscle mass can lead to the heart failure. Besides heart transplantation, there is no curative treatment option for the end-stage heart failure. In times of organ donor shortage, organ independent treatment modalities are needed. Left-ventricular assist devices are a promising therapy option, however, especially as destination therapy, limited by its side-effects like stroke, infections and bleedings. In recent years, several cardiac repair strategies including stem cell injection, cardiac progenitors or myocardial tissue engineering have been investigated. Recent improvements in cell biology allow for the differentiation of large amounts of cardiomyocytes derived from human induced pluripotent stem cells (iPSC). One of the cardiac repair strategies currently under evaluation is to transplant artificial heart tissue. Engineered heart tissue (EHT) is a three-dimensional in vitro created cardiomyocyte network, with functional properties of native heart tissue. We have created EHT-patches from hiPSC derived cardiomyocytes. Here we present a protocol for the induction of left ventricular myocardial cryoinjury in a guinea pig, followed by implantation of hiPSC derived EHT on the left ventricular wall.",
author = "Liesa Castro and Birgit Geertz and Marina Reinsch and B{\"u}lent Aksehirlioglu and Arne Hansen and Thomas Eschenhagen and Hermann Reichenspurner and Florian Weinberger and Simon Pecha",
year = "2019",
month = mar,
day = "18",
doi = "10.3791/58810",
language = "English",
journal = "JOVE-J VIS EXP",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "145",

}

RIS

TY - JOUR

T1 - Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model

AU - Castro, Liesa

AU - Geertz, Birgit

AU - Reinsch, Marina

AU - Aksehirlioglu, Bülent

AU - Hansen, Arne

AU - Eschenhagen, Thomas

AU - Reichenspurner, Hermann

AU - Weinberger, Florian

AU - Pecha, Simon

PY - 2019/3/18

Y1 - 2019/3/18

N2 - Due to the limited regeneration capacity of the heart in adult mammals, myocardial infarction results in an irreversible loss of cardiomyocytes. This loss of relevant amounts of heart muscle mass can lead to the heart failure. Besides heart transplantation, there is no curative treatment option for the end-stage heart failure. In times of organ donor shortage, organ independent treatment modalities are needed. Left-ventricular assist devices are a promising therapy option, however, especially as destination therapy, limited by its side-effects like stroke, infections and bleedings. In recent years, several cardiac repair strategies including stem cell injection, cardiac progenitors or myocardial tissue engineering have been investigated. Recent improvements in cell biology allow for the differentiation of large amounts of cardiomyocytes derived from human induced pluripotent stem cells (iPSC). One of the cardiac repair strategies currently under evaluation is to transplant artificial heart tissue. Engineered heart tissue (EHT) is a three-dimensional in vitro created cardiomyocyte network, with functional properties of native heart tissue. We have created EHT-patches from hiPSC derived cardiomyocytes. Here we present a protocol for the induction of left ventricular myocardial cryoinjury in a guinea pig, followed by implantation of hiPSC derived EHT on the left ventricular wall.

AB - Due to the limited regeneration capacity of the heart in adult mammals, myocardial infarction results in an irreversible loss of cardiomyocytes. This loss of relevant amounts of heart muscle mass can lead to the heart failure. Besides heart transplantation, there is no curative treatment option for the end-stage heart failure. In times of organ donor shortage, organ independent treatment modalities are needed. Left-ventricular assist devices are a promising therapy option, however, especially as destination therapy, limited by its side-effects like stroke, infections and bleedings. In recent years, several cardiac repair strategies including stem cell injection, cardiac progenitors or myocardial tissue engineering have been investigated. Recent improvements in cell biology allow for the differentiation of large amounts of cardiomyocytes derived from human induced pluripotent stem cells (iPSC). One of the cardiac repair strategies currently under evaluation is to transplant artificial heart tissue. Engineered heart tissue (EHT) is a three-dimensional in vitro created cardiomyocyte network, with functional properties of native heart tissue. We have created EHT-patches from hiPSC derived cardiomyocytes. Here we present a protocol for the induction of left ventricular myocardial cryoinjury in a guinea pig, followed by implantation of hiPSC derived EHT on the left ventricular wall.

U2 - 10.3791/58810

DO - 10.3791/58810

M3 - SCORING: Journal article

C2 - 30933073

JO - JOVE-J VIS EXP

JF - JOVE-J VIS EXP

SN - 1940-087X

IS - 145

ER -