Impact of homologous recombination on individual cellular radiosensitivity.
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Impact of homologous recombination on individual cellular radiosensitivity. / Koch, Kerstin; Wrona, Agnieszka; Dikomey, Ekkehard; Borgmann, Kerstin.
In: RADIOTHER ONCOL, Vol. 90, No. 2, 2, 2009, p. 265-272.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Impact of homologous recombination on individual cellular radiosensitivity.
AU - Koch, Kerstin
AU - Wrona, Agnieszka
AU - Dikomey, Ekkehard
AU - Borgmann, Kerstin
PY - 2009
Y1 - 2009
N2 - PURPOSE: Individual radiosensitivity as measured with in vitro irradiated lymphocytes using metaphase analysis can predict the risk of normal tissue effects after radiotherapy. This parameter is considered to be primarily determined by the cellular repair capacity of DNA double-strand breaks (DSBs). It is now tested to which extent this capacity also depends on homologous recombination (HR), which is a pathway available when cells are in S/G2 phase. METHODS: Experiments were performed with CHO K1 cells, in which HR was suppressed via knock-down of RAD51 using RNA interference (RNAi). RAD51 was measured via western and foci formation, cell survival by colony forming, DSBs by gammaH2AX foci formation, and chromosomal damage using PCC, G0 or G2 assay. RESULTS: In quiescent G1 cells DSB repair is completed 6h after irradiation. But there is still a substantial fraction of non-repaired DSBs. Most of these DSBs are repaired when G1 cells are stimulated into cell cycle. Suppression of HR by down-regulation of RAD51 did not affect this repair. In contrast, repair was inhibited when cells were irradiated in late S/G2. In line with these data down-regulation of HR did affect survival of cells irradiated in late S/G2, but not in G1. CONCLUSIONS: Individual radiosensitivity as measured for G0/1 cells using metaphase analysis does not depend on homologous recombination.
AB - PURPOSE: Individual radiosensitivity as measured with in vitro irradiated lymphocytes using metaphase analysis can predict the risk of normal tissue effects after radiotherapy. This parameter is considered to be primarily determined by the cellular repair capacity of DNA double-strand breaks (DSBs). It is now tested to which extent this capacity also depends on homologous recombination (HR), which is a pathway available when cells are in S/G2 phase. METHODS: Experiments were performed with CHO K1 cells, in which HR was suppressed via knock-down of RAD51 using RNA interference (RNAi). RAD51 was measured via western and foci formation, cell survival by colony forming, DSBs by gammaH2AX foci formation, and chromosomal damage using PCC, G0 or G2 assay. RESULTS: In quiescent G1 cells DSB repair is completed 6h after irradiation. But there is still a substantial fraction of non-repaired DSBs. Most of these DSBs are repaired when G1 cells are stimulated into cell cycle. Suppression of HR by down-regulation of RAD51 did not affect this repair. In contrast, repair was inhibited when cells were irradiated in late S/G2. In line with these data down-regulation of HR did affect survival of cells irradiated in late S/G2, but not in G1. CONCLUSIONS: Individual radiosensitivity as measured for G0/1 cells using metaphase analysis does not depend on homologous recombination.
M3 - SCORING: Zeitschriftenaufsatz
VL - 90
SP - 265
EP - 272
JO - RADIOTHER ONCOL
JF - RADIOTHER ONCOL
SN - 0167-8140
IS - 2
M1 - 2
ER -
