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Immunohistochemical detection of cytokeratins in the irradiated rat mandibular gland

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Immunohistochemical detection of cytokeratins in the irradiated rat mandibular gland. / Bartel-Friedrich, S; Friedrich, R E; Moll, R; Lautenschläger, C.

In: ANTICANCER RES, Vol. 19, No. 4A, 1999, p. 2405-9.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bartel-Friedrich, S, Friedrich, RE, Moll, R & Lautenschläger, C 1999, 'Immunohistochemical detection of cytokeratins in the irradiated rat mandibular gland', ANTICANCER RES, vol. 19, no. 4A, pp. 2405-9.

APA

Bartel-Friedrich, S., Friedrich, R. E., Moll, R., & Lautenschläger, C. (1999). Immunohistochemical detection of cytokeratins in the irradiated rat mandibular gland. ANTICANCER RES, 19(4A), 2405-9.

Vancouver

Bartel-Friedrich S, Friedrich RE, Moll R, Lautenschläger C. Immunohistochemical detection of cytokeratins in the irradiated rat mandibular gland. ANTICANCER RES. 1999;19(4A):2405-9.

Bibtex

@article{79746c1e19274f12b7ef982a65bc782f,
title = "Immunohistochemical detection of cytokeratins in the irradiated rat mandibular gland",
abstract = "UNLABELLED: BACKGROUND/MATERIAL AND METHODS: The present study is a prerequisite for the exploration of the cytokeratin (CK) profile in rat salivary gland tumors. In 59 rat mandibular glands we investigated the distribution of CK by immunohistochemical analysis. The animals differed in age and pretreatment status (irradiation versus no irradiation).RESULTS: The monoclonal antibodies (CKE3, Ks13.1, NCL5D3, K8.12; against CK 17, CK 13, CK 8 and CK 13/15/16, respectively) identified different epithelial structures in rat salivary gland tissue, including intercalated duct cells (ICD), striated duct cells (SD), granular convoluted tubules (GTC), excretory duct cells (ECD) and myoepithelial cells (MC). As typical results, CKE3 usually stained the ICD, SD and ECD moderately to strongly and stained the GTC slightly. K8.12 staining was restricted to ECD and MC. Differences in immunoreactivity were seen between irradiated and non-irradiated glands, predominantly with stronger staining in the irradiated group.CONCLUSIONS: Previous radiation has to be considered when interpreting immunohistochemical stainings of salivary gland tissue, especially in tumor differentiation studies following irradiation.",
keywords = "Aging, Animals, Immunohistochemistry, Keratins, Rats, Salivary Gland Neoplasms, Submandibular Gland, Time Factors, Tumor Markers, Biological",
author = "S Bartel-Friedrich and Friedrich, {R E} and R Moll and C Lautenschl{\"a}ger",
year = "1999",
language = "English",
volume = "19",
pages = "2405--9",
journal = "ANTICANCER RES",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "4A",

}

RIS

TY - JOUR

T1 - Immunohistochemical detection of cytokeratins in the irradiated rat mandibular gland

AU - Bartel-Friedrich, S

AU - Friedrich, R E

AU - Moll, R

AU - Lautenschläger, C

PY - 1999

Y1 - 1999

N2 - UNLABELLED: BACKGROUND/MATERIAL AND METHODS: The present study is a prerequisite for the exploration of the cytokeratin (CK) profile in rat salivary gland tumors. In 59 rat mandibular glands we investigated the distribution of CK by immunohistochemical analysis. The animals differed in age and pretreatment status (irradiation versus no irradiation).RESULTS: The monoclonal antibodies (CKE3, Ks13.1, NCL5D3, K8.12; against CK 17, CK 13, CK 8 and CK 13/15/16, respectively) identified different epithelial structures in rat salivary gland tissue, including intercalated duct cells (ICD), striated duct cells (SD), granular convoluted tubules (GTC), excretory duct cells (ECD) and myoepithelial cells (MC). As typical results, CKE3 usually stained the ICD, SD and ECD moderately to strongly and stained the GTC slightly. K8.12 staining was restricted to ECD and MC. Differences in immunoreactivity were seen between irradiated and non-irradiated glands, predominantly with stronger staining in the irradiated group.CONCLUSIONS: Previous radiation has to be considered when interpreting immunohistochemical stainings of salivary gland tissue, especially in tumor differentiation studies following irradiation.

AB - UNLABELLED: BACKGROUND/MATERIAL AND METHODS: The present study is a prerequisite for the exploration of the cytokeratin (CK) profile in rat salivary gland tumors. In 59 rat mandibular glands we investigated the distribution of CK by immunohistochemical analysis. The animals differed in age and pretreatment status (irradiation versus no irradiation).RESULTS: The monoclonal antibodies (CKE3, Ks13.1, NCL5D3, K8.12; against CK 17, CK 13, CK 8 and CK 13/15/16, respectively) identified different epithelial structures in rat salivary gland tissue, including intercalated duct cells (ICD), striated duct cells (SD), granular convoluted tubules (GTC), excretory duct cells (ECD) and myoepithelial cells (MC). As typical results, CKE3 usually stained the ICD, SD and ECD moderately to strongly and stained the GTC slightly. K8.12 staining was restricted to ECD and MC. Differences in immunoreactivity were seen between irradiated and non-irradiated glands, predominantly with stronger staining in the irradiated group.CONCLUSIONS: Previous radiation has to be considered when interpreting immunohistochemical stainings of salivary gland tissue, especially in tumor differentiation studies following irradiation.

KW - Aging

KW - Animals

KW - Immunohistochemistry

KW - Keratins

KW - Rats

KW - Salivary Gland Neoplasms

KW - Submandibular Gland

KW - Time Factors

KW - Tumor Markers, Biological

M3 - SCORING: Journal article

C2 - 10470166

VL - 19

SP - 2405

EP - 2409

JO - ANTICANCER RES

JF - ANTICANCER RES

SN - 0250-7005

IS - 4A

ER -