Immunohistochemical Analysis of Transcription Factors and Markers of Epithelial-Mesenchymal Transition (EMT) in Human Tumors
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Immunohistochemical Analysis of Transcription Factors and Markers of Epithelial-Mesenchymal Transition (EMT) in Human Tumors. / Ghulam, Jasamin; Stuerken, Christine; Wicklein, Daniel; Pries, Ralph; Wollenberg, Barbara; Schumacher, Udo.
In: ANTICANCER RES, Vol. 39, No. 10, 10.2019, p. 5437-5448.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Immunohistochemical Analysis of Transcription Factors and Markers of Epithelial-Mesenchymal Transition (EMT) in Human Tumors
AU - Ghulam, Jasamin
AU - Stuerken, Christine
AU - Wicklein, Daniel
AU - Pries, Ralph
AU - Wollenberg, Barbara
AU - Schumacher, Udo
N1 - Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
PY - 2019/10
Y1 - 2019/10
N2 - BACKGROUND/AIM: Epithelial-mesenchymal transition (EMT) is a key multi-step process which enables cancer cells to detach from the epithelial primary tumor mass and allows them to metastasize to distant organs. We immunohistochemically analyzed the expression of the transcription factors (TWIST-1, SLUG, ZEB1, ZEB2) and components of the extracellular matrix (laminin-5, fibronectin) which influence the EMT.MATERIALS AND METHODS: Primary human breast (MDA-MB-231), colon (HT29, HCT116), ovarian (SKOV3, OVCAR3) and head and neck squamous cell carcinoma cell lines (UTSCC2, UTSCC24A) grown as xenografts were immunohistochemically analyzed in vitro and in vivo.RESULTS: A high SLUG expression was observed in every cancer entity both in vitro and in vivo. ZEB1 and ZEB2 showed a high in vivo expression especially in SKOV3 and in in vitro grown MDA-MB-231 cells.CONCLUSION: SLUG expression showed the highest expression in all cancer entities investigated. Hence, it presumably represents the master regulator of EMT in these metastatic tumor entities.
AB - BACKGROUND/AIM: Epithelial-mesenchymal transition (EMT) is a key multi-step process which enables cancer cells to detach from the epithelial primary tumor mass and allows them to metastasize to distant organs. We immunohistochemically analyzed the expression of the transcription factors (TWIST-1, SLUG, ZEB1, ZEB2) and components of the extracellular matrix (laminin-5, fibronectin) which influence the EMT.MATERIALS AND METHODS: Primary human breast (MDA-MB-231), colon (HT29, HCT116), ovarian (SKOV3, OVCAR3) and head and neck squamous cell carcinoma cell lines (UTSCC2, UTSCC24A) grown as xenografts were immunohistochemically analyzed in vitro and in vivo.RESULTS: A high SLUG expression was observed in every cancer entity both in vitro and in vivo. ZEB1 and ZEB2 showed a high in vivo expression especially in SKOV3 and in in vitro grown MDA-MB-231 cells.CONCLUSION: SLUG expression showed the highest expression in all cancer entities investigated. Hence, it presumably represents the master regulator of EMT in these metastatic tumor entities.
KW - Biomarkers, Tumor/metabolism
KW - Cell Line, Tumor
KW - Epithelial-Mesenchymal Transition/physiology
KW - Extracellular Matrix/metabolism
KW - HCT116 Cells
KW - HT29 Cells
KW - Humans
KW - Immunohistochemistry/methods
KW - Transcription Factors/metabolism
KW - Zinc Finger E-box Binding Homeobox 2/metabolism
KW - Zinc Finger E-box-Binding Homeobox 1/metabolism
U2 - 10.21873/anticanres.13737
DO - 10.21873/anticanres.13737
M3 - SCORING: Journal article
C2 - 31570438
VL - 39
SP - 5437
EP - 5448
JO - ANTICANCER RES
JF - ANTICANCER RES
SN - 0250-7005
IS - 10
ER -
