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Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice

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Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice. / Allweiss, Lena; Lütgehetmann, Marc; Dandri, Maura.

In: Methods Mol Biol, Vol. 1540, 2017, p. 135-142.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Allweiss, L, Lütgehetmann, M & Dandri, M 2017, 'Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice', Methods Mol Biol, vol. 1540, pp. 135-142. https://doi.org/10.1007/978-1-4939-6700-1_11

APA

Allweiss, L., Lütgehetmann, M., & Dandri, M. (2017). Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice. Methods Mol Biol, 1540, 135-142. https://doi.org/10.1007/978-1-4939-6700-1_11

Vancouver

Allweiss L, Lütgehetmann M, Dandri M. Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice. Methods Mol Biol. 2017;1540:135-142. https://doi.org/10.1007/978-1-4939-6700-1_11

Bibtex

@article{a07534f78778494598cb2daf3daddd69,
title = "Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice",
abstract = "The hepatitis B virus (HBV) is the causative agent for chronic hepatitis B infection, which affects an estimate of 240 million people worldwide and puts them at risk of developing terminal liver disease. The life cycle of the virus and its interactions with the host immune system are still incompletely understood, and currently available treatment options rarely achieve a cure. Therefore, basic research and new drug development are needed. One parameter for measuring the intrahepatic activity of the virus is monitoring the production of the HBV core antigen (HBcAg), which not only serves as the main structural protein of its nucleocapsid but is also recruited to the covalently closed circular DNA (cccDNA), the nuclear HBV genome responsible for infection persistence. Here, we report a sensitive immunofluorescence staining method to detect HBcAg in cryopreserved liver sections. The method combines conventional immunofluorescence staining procedures with the Tyramide Signal Amplification (TSA) system.",
keywords = "Animals, Fluorescent Antibody Technique, Hepatitis B Core Antigens, Hepatitis B virus, Hepatitis B, Chronic, Humans, Immunohistochemistry, Mice, Microscopy, Fluorescence, Transplantation Chimera, Journal Article",
author = "Lena Allweiss and Marc L{\"u}tgehetmann and Maura Dandri",
year = "2017",
doi = "10.1007/978-1-4939-6700-1_11",
language = "English",
volume = "1540",
pages = "135--142",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Immunofluorescent Staining for the Detection of the Hepatitis B Core Antigen in Frozen Liver Sections of Human Liver Chimeric Mice

AU - Allweiss, Lena

AU - Lütgehetmann, Marc

AU - Dandri, Maura

PY - 2017

Y1 - 2017

N2 - The hepatitis B virus (HBV) is the causative agent for chronic hepatitis B infection, which affects an estimate of 240 million people worldwide and puts them at risk of developing terminal liver disease. The life cycle of the virus and its interactions with the host immune system are still incompletely understood, and currently available treatment options rarely achieve a cure. Therefore, basic research and new drug development are needed. One parameter for measuring the intrahepatic activity of the virus is monitoring the production of the HBV core antigen (HBcAg), which not only serves as the main structural protein of its nucleocapsid but is also recruited to the covalently closed circular DNA (cccDNA), the nuclear HBV genome responsible for infection persistence. Here, we report a sensitive immunofluorescence staining method to detect HBcAg in cryopreserved liver sections. The method combines conventional immunofluorescence staining procedures with the Tyramide Signal Amplification (TSA) system.

AB - The hepatitis B virus (HBV) is the causative agent for chronic hepatitis B infection, which affects an estimate of 240 million people worldwide and puts them at risk of developing terminal liver disease. The life cycle of the virus and its interactions with the host immune system are still incompletely understood, and currently available treatment options rarely achieve a cure. Therefore, basic research and new drug development are needed. One parameter for measuring the intrahepatic activity of the virus is monitoring the production of the HBV core antigen (HBcAg), which not only serves as the main structural protein of its nucleocapsid but is also recruited to the covalently closed circular DNA (cccDNA), the nuclear HBV genome responsible for infection persistence. Here, we report a sensitive immunofluorescence staining method to detect HBcAg in cryopreserved liver sections. The method combines conventional immunofluorescence staining procedures with the Tyramide Signal Amplification (TSA) system.

KW - Animals

KW - Fluorescent Antibody Technique

KW - Hepatitis B Core Antigens

KW - Hepatitis B virus

KW - Hepatitis B, Chronic

KW - Humans

KW - Immunohistochemistry

KW - Mice

KW - Microscopy, Fluorescence

KW - Transplantation Chimera

KW - Journal Article

U2 - 10.1007/978-1-4939-6700-1_11

DO - 10.1007/978-1-4939-6700-1_11

M3 - SCORING: Journal article

C2 - 27975313

VL - 1540

SP - 135

EP - 142

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -