Immunoexpression analysis and prognostic value of BLCAP in breast cancer.
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Immunoexpression analysis and prognostic value of BLCAP in breast cancer. / Gromova, Irina; Gromov, Pavel; Kroman, Niels; Wielenga, Vera Timmermans; Simon, Ronald; Sauter, Guido; Moreira, José M A.
In: PLOS ONE, Vol. 7, No. 9, 9, 2012, p. 45967.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Immunoexpression analysis and prognostic value of BLCAP in breast cancer.
AU - Gromova, Irina
AU - Gromov, Pavel
AU - Kroman, Niels
AU - Wielenga, Vera Timmermans
AU - Simon, Ronald
AU - Sauter, Guido
AU - Moreira, José M A
PY - 2012
Y1 - 2012
N2 - Bladder Cancer Associated Protein (BLCAP, formerly Bc10), was identified by our laboratory as being down-regulated in bladder cancer with progression. BLCAP is ubiquitously expressed in different tissues, and several studies have found differential expression of BLCAP in various cancer types, such as cervical and renal cancer, as well as human tongue carcinoma and osteosarcoma. Here we report the first study of the expression patterns of BLCAP in breast tissue. We analyzed by immunohistochemistry tissue sections of normal and malignant specimens collected from 123 clinical high-risk breast cancer patients within the Danish Center for Translational Breast Cancer Research (DCTB) prospective study dataset. The staining pattern, the distribution of the immunostaining, and its intensity were studied in detail. We observed weak immunoreactivity for BLCAP in mammary epithelial cells, almost exclusively localizing to the cytoplasm and found that levels of expression of BLCAP were generally higher in malignant cells as compared to normal cells. Quantitative IHC analysis of BLCAP expression in breast tissues confirmed this differential BLCAP expression in tumor cells, and we could establish, in a 62-patient sample matched cohort, that immunostaining intensity for BLCAP was increased in tumors relative to normal tissue, in more than 45% of the cases examined, indicating that BLCAP may be of value as a marker for breast cancer. We also analyzed BLCAP expression and prognostic value using a set of tissue microarrays comprising an independent cohort of 2,197 breast cancer patients for which we had follow-up clinical information.
AB - Bladder Cancer Associated Protein (BLCAP, formerly Bc10), was identified by our laboratory as being down-regulated in bladder cancer with progression. BLCAP is ubiquitously expressed in different tissues, and several studies have found differential expression of BLCAP in various cancer types, such as cervical and renal cancer, as well as human tongue carcinoma and osteosarcoma. Here we report the first study of the expression patterns of BLCAP in breast tissue. We analyzed by immunohistochemistry tissue sections of normal and malignant specimens collected from 123 clinical high-risk breast cancer patients within the Danish Center for Translational Breast Cancer Research (DCTB) prospective study dataset. The staining pattern, the distribution of the immunostaining, and its intensity were studied in detail. We observed weak immunoreactivity for BLCAP in mammary epithelial cells, almost exclusively localizing to the cytoplasm and found that levels of expression of BLCAP were generally higher in malignant cells as compared to normal cells. Quantitative IHC analysis of BLCAP expression in breast tissues confirmed this differential BLCAP expression in tumor cells, and we could establish, in a 62-patient sample matched cohort, that immunostaining intensity for BLCAP was increased in tumors relative to normal tissue, in more than 45% of the cases examined, indicating that BLCAP may be of value as a marker for breast cancer. We also analyzed BLCAP expression and prognostic value using a set of tissue microarrays comprising an independent cohort of 2,197 breast cancer patients for which we had follow-up clinical information.
KW - Adult
KW - Humans
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Prospective Studies
KW - Cohort Studies
KW - Prognosis
KW - Disease Progression
KW - Denmark
KW - Tumor Markers, Biological/metabolism
KW - Immunohistochemistry/methods
KW - Gene Expression Regulation, Neoplastic
KW - Cytoplasm/metabolism
KW - Electrophoresis, Gel, Two-Dimensional/methods
KW - Mass Spectrometry/methods
KW - Breast Neoplasms/immunology
KW - Neoplasm Proteins/biosynthesis/genetics
KW - Adult
KW - Humans
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Prospective Studies
KW - Cohort Studies
KW - Prognosis
KW - Disease Progression
KW - Denmark
KW - Tumor Markers, Biological/metabolism
KW - Immunohistochemistry/methods
KW - Gene Expression Regulation, Neoplastic
KW - Cytoplasm/metabolism
KW - Electrophoresis, Gel, Two-Dimensional/methods
KW - Mass Spectrometry/methods
KW - Breast Neoplasms/immunology
KW - Neoplasm Proteins/biosynthesis/genetics
U2 - 10.1371/journal.pone.0045967
DO - 10.1371/journal.pone.0045967
M3 - SCORING: Journal article
VL - 7
SP - 45967
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 9
M1 - 9
ER -
