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Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes.

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Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes. / Paul, D; Höhne, M; Pinkert, C; Piasecki, Angelika; Ummelmann, E; Brinster, R L.

In: EXP CELL RES, Vol. 175, No. 2, 2, 1988, p. 354-362.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Paul, D, Höhne, M, Pinkert, C, Piasecki, A, Ummelmann, E & Brinster, RL 1988, 'Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes.', EXP CELL RES, vol. 175, no. 2, 2, pp. 354-362. <http://www.ncbi.nlm.nih.gov/pubmed/3282899?dopt=Citation>

APA

Paul, D., Höhne, M., Pinkert, C., Piasecki, A., Ummelmann, E., & Brinster, R. L. (1988). Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes. EXP CELL RES, 175(2), 354-362. [2]. http://www.ncbi.nlm.nih.gov/pubmed/3282899?dopt=Citation

Vancouver

Paul D, Höhne M, Pinkert C, Piasecki A, Ummelmann E, Brinster RL. Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes. EXP CELL RES. 1988;175(2):354-362. 2.

Bibtex

@article{f67f868b3b8948d7a74683f0b1efd3ac,
title = "Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes.",
abstract = "Hepatocytes of transgenic mouse fetuses harboring SV40 virus transforming gene sequences in the SV delta e-MGH fusion gene construct 202 driven by the mouse metallothionein (MT-I) enhancer [R. D. Palmiter, H. Y. Chen, A. Messing, and R. L. Brinster (1985) Nature (London) 316, 457-460] were cultured at Day 19 of gestation and established as a differentiated line expressing albumin and alpha-fetoprotein (AFP) mRNAs. Hepatocyte line FMH-202 contains integrated SV40 sequences, expresses SV40 T-antigen genes, and exhibits unlimited growth potential because it has been cultured 18 months without apparent decrease in cell viability or in growth rate that could suggest the occurrence of a crisis period. Immortalized cells multiply in chemically defined medium deficient in arginine with transferrin plus insulin, whereas EGF, insulin, and transferrin are obligatory requirements for fetal or newborn mouse hepatocyte multiplication in primary cultures. Cells did not grow in agar and were not tumorigenic in nude mice. Their immortalized, nonmalignant phenotype was further documented by low saturation densities of confluent monolayers showing no overgrowth, and by growth arrest in the absence of insulin with subsequent induction of DNA synthesis and resumption of cell growth in response to insulin. Thus, it appears that immortalized SV40 T-antigen-expressing hepatocytes are present in the liver of the transgenic mice. However, at later points in liver development the transforming activity of T-antigen becomes apparent and leads to hepatocellular carcinoma formation in vivo.",
keywords = "Animals, Mice, Mice, Transgenic, Cell Differentiation, Cell Division, Fetus, Insulin/metabolism, Culture Media, Genes, Viral, Cell Survival, Liver/*cytology, Antigens, Polyomavirus Transforming/*genetics, Arginine/biosynthesis, *Cell Line, DNA, Viral/genetics, Nucleic Acid Hybridization, Transferrin/metabolism, Animals, Mice, Mice, Transgenic, Cell Differentiation, Cell Division, Fetus, Insulin/metabolism, Culture Media, Genes, Viral, Cell Survival, Liver/*cytology, Antigens, Polyomavirus Transforming/*genetics, Arginine/biosynthesis, *Cell Line, DNA, Viral/genetics, Nucleic Acid Hybridization, Transferrin/metabolism",
author = "D Paul and M H{\"o}hne and C Pinkert and Angelika Piasecki and E Ummelmann and Brinster, {R L}",
year = "1988",
language = "English",
volume = "175",
pages = "354--362",
journal = "EXP CELL RES",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes.

AU - Paul, D

AU - Höhne, M

AU - Pinkert, C

AU - Piasecki, Angelika

AU - Ummelmann, E

AU - Brinster, R L

PY - 1988

Y1 - 1988

N2 - Hepatocytes of transgenic mouse fetuses harboring SV40 virus transforming gene sequences in the SV delta e-MGH fusion gene construct 202 driven by the mouse metallothionein (MT-I) enhancer [R. D. Palmiter, H. Y. Chen, A. Messing, and R. L. Brinster (1985) Nature (London) 316, 457-460] were cultured at Day 19 of gestation and established as a differentiated line expressing albumin and alpha-fetoprotein (AFP) mRNAs. Hepatocyte line FMH-202 contains integrated SV40 sequences, expresses SV40 T-antigen genes, and exhibits unlimited growth potential because it has been cultured 18 months without apparent decrease in cell viability or in growth rate that could suggest the occurrence of a crisis period. Immortalized cells multiply in chemically defined medium deficient in arginine with transferrin plus insulin, whereas EGF, insulin, and transferrin are obligatory requirements for fetal or newborn mouse hepatocyte multiplication in primary cultures. Cells did not grow in agar and were not tumorigenic in nude mice. Their immortalized, nonmalignant phenotype was further documented by low saturation densities of confluent monolayers showing no overgrowth, and by growth arrest in the absence of insulin with subsequent induction of DNA synthesis and resumption of cell growth in response to insulin. Thus, it appears that immortalized SV40 T-antigen-expressing hepatocytes are present in the liver of the transgenic mice. However, at later points in liver development the transforming activity of T-antigen becomes apparent and leads to hepatocellular carcinoma formation in vivo.

AB - Hepatocytes of transgenic mouse fetuses harboring SV40 virus transforming gene sequences in the SV delta e-MGH fusion gene construct 202 driven by the mouse metallothionein (MT-I) enhancer [R. D. Palmiter, H. Y. Chen, A. Messing, and R. L. Brinster (1985) Nature (London) 316, 457-460] were cultured at Day 19 of gestation and established as a differentiated line expressing albumin and alpha-fetoprotein (AFP) mRNAs. Hepatocyte line FMH-202 contains integrated SV40 sequences, expresses SV40 T-antigen genes, and exhibits unlimited growth potential because it has been cultured 18 months without apparent decrease in cell viability or in growth rate that could suggest the occurrence of a crisis period. Immortalized cells multiply in chemically defined medium deficient in arginine with transferrin plus insulin, whereas EGF, insulin, and transferrin are obligatory requirements for fetal or newborn mouse hepatocyte multiplication in primary cultures. Cells did not grow in agar and were not tumorigenic in nude mice. Their immortalized, nonmalignant phenotype was further documented by low saturation densities of confluent monolayers showing no overgrowth, and by growth arrest in the absence of insulin with subsequent induction of DNA synthesis and resumption of cell growth in response to insulin. Thus, it appears that immortalized SV40 T-antigen-expressing hepatocytes are present in the liver of the transgenic mice. However, at later points in liver development the transforming activity of T-antigen becomes apparent and leads to hepatocellular carcinoma formation in vivo.

KW - Animals

KW - Mice

KW - Mice, Transgenic

KW - Cell Differentiation

KW - Cell Division

KW - Fetus

KW - Insulin/metabolism

KW - Culture Media

KW - Genes, Viral

KW - Cell Survival

KW - Liver/cytology

KW - Antigens, Polyomavirus Transforming/genetics

KW - Arginine/biosynthesis

KW - Cell Line

KW - DNA, Viral/genetics

KW - Nucleic Acid Hybridization

KW - Transferrin/metabolism

KW - Animals

KW - Mice

KW - Mice, Transgenic

KW - Cell Differentiation

KW - Cell Division

KW - Fetus

KW - Insulin/metabolism

KW - Culture Media

KW - Genes, Viral

KW - Cell Survival

KW - Liver/cytology

KW - Antigens, Polyomavirus Transforming/genetics

KW - Arginine/biosynthesis

KW - Cell Line

KW - DNA, Viral/genetics

KW - Nucleic Acid Hybridization

KW - Transferrin/metabolism

M3 - SCORING: Journal article

VL - 175

SP - 354

EP - 362

JO - EXP CELL RES

JF - EXP CELL RES

SN - 0014-4827

IS - 2

M1 - 2

ER -