Immediate antigen-specific effector functions by TCR-transgenic CD8+ NKT cells

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Immediate antigen-specific effector functions by TCR-transgenic CD8+ NKT cells. / Wingender, Gerhard; Berg, Martina; Jüngerkes, Frank; Diehl, Linda; Sullivan, Barbara A; Kronenberg, Mitchell; Limmer, Andreas; Knolle, Percy A.

In: EUR J IMMUNOL, Vol. 36, No. 3, 03.2006, p. 570-82.

Research output: SCORING: Contribution to journalSCORING: Journal articleResearchpeer-review

Harvard

Wingender, G, Berg, M, Jüngerkes, F, Diehl, L, Sullivan, BA, Kronenberg, M, Limmer, A & Knolle, PA 2006, 'Immediate antigen-specific effector functions by TCR-transgenic CD8+ NKT cells', EUR J IMMUNOL, vol. 36, no. 3, pp. 570-82. https://doi.org/10.1002/eji.200535461

APA

Wingender, G., Berg, M., Jüngerkes, F., Diehl, L., Sullivan, B. A., Kronenberg, M., Limmer, A., & Knolle, P. A. (2006). Immediate antigen-specific effector functions by TCR-transgenic CD8+ NKT cells. EUR J IMMUNOL, 36(3), 570-82. https://doi.org/10.1002/eji.200535461

Vancouver

Wingender G, Berg M, Jüngerkes F, Diehl L, Sullivan BA, Kronenberg M et al. Immediate antigen-specific effector functions by TCR-transgenic CD8+ NKT cells. EUR J IMMUNOL. 2006 Mar;36(3):570-82. https://doi.org/10.1002/eji.200535461

Bibtex

@article{f4ea58e9be0b44e09df68d2f388cd8b1,
title = "Immediate antigen-specific effector functions by TCR-transgenic CD8+ NKT cells",
abstract = "Only recently have natural antigens for CD1d-dependent, invariant Valpha14+ natural killer T (iNKT) cells been identified. Similar data for CD1d-independent and CD8+ NKT cell populations are still missing. Here, we show that the MHC class I-restricted CD8+ TCR-transgenic mouse lines OT-I, P14 and H-Y contain a significant proportion of transgenic CD8+ NK1.1+ T cells. In liver, most of NK1.1+ T cells express CD8alphaalpha homodimers. Transgenic NKT cells did not bind invariant Valpha14-to-Jalpha18 TCR rearrangement (Valpha14i)-specific CD1d/alpha-galactosylceramide tetramers and the frequency of iNKT cells was severely reduced. The activated cell surface phenotype and the distribution of transgenic NKT cells in vivo were similar to that reported for iNKT cells. The OT-I and P14 CD8+ NKT cells recognized their cognate antigen in the context of H2-Kb and produced cytokines shortly after TCR stimulation. Importantly, transgenic NKT cells exerted immediate antigen-specific cytotoxicity in vitro and in vivo. Our results demonstrate the presence of transgenic CD8+ NKT cells in MHC class I-restricted TCR-transgenic animals, which are endowed with rapid antigen-specific effector functions. These data imply that experiments studying naive T cell function in TCR-transgenic animals should be interpreted with caution, and that such animals could be utilized for studying CD8+ NKT cell function in an antigen-specific manner.",
keywords = "Animals, Antigen Presentation, Antigens, CD1, Antigens, CD1d, Antigens, CD8, Antigens, Ly, Antigens, Surface, CD8-Positive T-Lymphocytes, Galactosylceramides, Gene Rearrangement, T-Lymphocyte, H-2 Antigens, Killer Cells, Natural, Lectins, C-Type, Liver, Lymphocyte Activation, Mice, Mice, Transgenic, NK Cell Lectin-Like Receptor Subfamily B, Proteins, Receptors, Antigen, T-Cell, alpha-beta",
author = "Gerhard Wingender and Martina Berg and Frank J{\"u}ngerkes and Linda Diehl and Sullivan, {Barbara A} and Mitchell Kronenberg and Andreas Limmer and Knolle, {Percy A}",
year = "2006",
month = mar,
doi = "10.1002/eji.200535461",
language = "English",
volume = "36",
pages = "570--82",
journal = "EUR J IMMUNOL",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag GmbH",
number = "3",

}

RIS

TY - JOUR

T1 - Immediate antigen-specific effector functions by TCR-transgenic CD8+ NKT cells

AU - Wingender, Gerhard

AU - Berg, Martina

AU - Jüngerkes, Frank

AU - Diehl, Linda

AU - Sullivan, Barbara A

AU - Kronenberg, Mitchell

AU - Limmer, Andreas

AU - Knolle, Percy A

PY - 2006/3

Y1 - 2006/3

N2 - Only recently have natural antigens for CD1d-dependent, invariant Valpha14+ natural killer T (iNKT) cells been identified. Similar data for CD1d-independent and CD8+ NKT cell populations are still missing. Here, we show that the MHC class I-restricted CD8+ TCR-transgenic mouse lines OT-I, P14 and H-Y contain a significant proportion of transgenic CD8+ NK1.1+ T cells. In liver, most of NK1.1+ T cells express CD8alphaalpha homodimers. Transgenic NKT cells did not bind invariant Valpha14-to-Jalpha18 TCR rearrangement (Valpha14i)-specific CD1d/alpha-galactosylceramide tetramers and the frequency of iNKT cells was severely reduced. The activated cell surface phenotype and the distribution of transgenic NKT cells in vivo were similar to that reported for iNKT cells. The OT-I and P14 CD8+ NKT cells recognized their cognate antigen in the context of H2-Kb and produced cytokines shortly after TCR stimulation. Importantly, transgenic NKT cells exerted immediate antigen-specific cytotoxicity in vitro and in vivo. Our results demonstrate the presence of transgenic CD8+ NKT cells in MHC class I-restricted TCR-transgenic animals, which are endowed with rapid antigen-specific effector functions. These data imply that experiments studying naive T cell function in TCR-transgenic animals should be interpreted with caution, and that such animals could be utilized for studying CD8+ NKT cell function in an antigen-specific manner.

AB - Only recently have natural antigens for CD1d-dependent, invariant Valpha14+ natural killer T (iNKT) cells been identified. Similar data for CD1d-independent and CD8+ NKT cell populations are still missing. Here, we show that the MHC class I-restricted CD8+ TCR-transgenic mouse lines OT-I, P14 and H-Y contain a significant proportion of transgenic CD8+ NK1.1+ T cells. In liver, most of NK1.1+ T cells express CD8alphaalpha homodimers. Transgenic NKT cells did not bind invariant Valpha14-to-Jalpha18 TCR rearrangement (Valpha14i)-specific CD1d/alpha-galactosylceramide tetramers and the frequency of iNKT cells was severely reduced. The activated cell surface phenotype and the distribution of transgenic NKT cells in vivo were similar to that reported for iNKT cells. The OT-I and P14 CD8+ NKT cells recognized their cognate antigen in the context of H2-Kb and produced cytokines shortly after TCR stimulation. Importantly, transgenic NKT cells exerted immediate antigen-specific cytotoxicity in vitro and in vivo. Our results demonstrate the presence of transgenic CD8+ NKT cells in MHC class I-restricted TCR-transgenic animals, which are endowed with rapid antigen-specific effector functions. These data imply that experiments studying naive T cell function in TCR-transgenic animals should be interpreted with caution, and that such animals could be utilized for studying CD8+ NKT cell function in an antigen-specific manner.

KW - Animals

KW - Antigen Presentation

KW - Antigens, CD1

KW - Antigens, CD1d

KW - Antigens, CD8

KW - Antigens, Ly

KW - Antigens, Surface

KW - CD8-Positive T-Lymphocytes

KW - Galactosylceramides

KW - Gene Rearrangement, T-Lymphocyte

KW - H-2 Antigens

KW - Killer Cells, Natural

KW - Lectins, C-Type

KW - Liver

KW - Lymphocyte Activation

KW - Mice

KW - Mice, Transgenic

KW - NK Cell Lectin-Like Receptor Subfamily B

KW - Proteins

KW - Receptors, Antigen, T-Cell, alpha-beta

U2 - 10.1002/eji.200535461

DO - 10.1002/eji.200535461

M3 - SCORING: Journal article

C2 - 16506291

VL - 36

SP - 570

EP - 582

JO - EUR J IMMUNOL

JF - EUR J IMMUNOL

SN - 0014-2980

IS - 3

ER -