Imaging analysis of nuclear antiviral factors through direct detection of incoming adenovirus genome complexes
Standard
Imaging analysis of nuclear antiviral factors through direct detection of incoming adenovirus genome complexes. / Komatsu, Tetsuro; Will, Hans; Nagata, Kyosuke; Wodrich, Harald.
In: BIOCHEM BIOPH RES CO, Vol. 473, No. 1, 22.04.2016, p. 200-5.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Imaging analysis of nuclear antiviral factors through direct detection of incoming adenovirus genome complexes
AU - Komatsu, Tetsuro
AU - Will, Hans
AU - Nagata, Kyosuke
AU - Wodrich, Harald
N1 - Copyright © 2016 Elsevier Inc. All rights reserved.
PY - 2016/4/22
Y1 - 2016/4/22
N2 - Recent studies involving several viral systems have highlighted the importance of cellular intrinsic defense mechanisms through nuclear antiviral proteins that restrict viral propagation. These factors include among others components of PML nuclear bodies, the nuclear DNA sensor IFI16, and a potential restriction factor PHF13/SPOC1. For several nuclear replicating DNA viruses, it was shown that these factors sense and target viral genomes immediately upon nuclear import. In contrast to the anticipated view, we recently found that incoming adenoviral genomes are not targeted by PML nuclear bodies. Here we further explored cellular responses against adenoviral infection by focusing on specific conditions as well as additional nuclear antiviral factors. In line with our previous findings, we show that neither interferon treatment nor the use of specific isoforms of PML nuclear body components results in co-localization between incoming adenoviral genomes and the subnuclear domains. Furthermore, our imaging analyses indicated that neither IFI16 nor PHF13/SPOC1 are likely to target incoming adenoviral genomes. Thus our findings suggest that incoming adenoviral genomes may be able to escape from a large repertoire of nuclear antiviral mechanisms, providing a rationale for the efficient initiation of lytic replication cycle.
AB - Recent studies involving several viral systems have highlighted the importance of cellular intrinsic defense mechanisms through nuclear antiviral proteins that restrict viral propagation. These factors include among others components of PML nuclear bodies, the nuclear DNA sensor IFI16, and a potential restriction factor PHF13/SPOC1. For several nuclear replicating DNA viruses, it was shown that these factors sense and target viral genomes immediately upon nuclear import. In contrast to the anticipated view, we recently found that incoming adenoviral genomes are not targeted by PML nuclear bodies. Here we further explored cellular responses against adenoviral infection by focusing on specific conditions as well as additional nuclear antiviral factors. In line with our previous findings, we show that neither interferon treatment nor the use of specific isoforms of PML nuclear body components results in co-localization between incoming adenoviral genomes and the subnuclear domains. Furthermore, our imaging analyses indicated that neither IFI16 nor PHF13/SPOC1 are likely to target incoming adenoviral genomes. Thus our findings suggest that incoming adenoviral genomes may be able to escape from a large repertoire of nuclear antiviral mechanisms, providing a rationale for the efficient initiation of lytic replication cycle.
KW - Adenoviridae
KW - Adenoviridae Infections
KW - Cell Line, Tumor
KW - DNA-Binding Proteins
KW - Fluorescent Antibody Technique, Indirect
KW - Genome, Viral
KW - Host-Pathogen Interactions
KW - Humans
KW - Interferons
KW - Microscopy, Fluorescence
KW - Neutrophils
KW - Nuclear Proteins
KW - Phosphoproteins
KW - Transcription Factors
KW - Virus Replication
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.bbrc.2016.03.078
DO - 10.1016/j.bbrc.2016.03.078
M3 - SCORING: Journal article
C2 - 27012198
VL - 473
SP - 200
EP - 205
JO - BIOCHEM BIOPH RES CO
JF - BIOCHEM BIOPH RES CO
SN - 0006-291X
IS - 1
ER -