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IgG subclass switching and clonal expansion in cutaneous melanoma and normal skin

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IgG subclass switching and clonal expansion in cutaneous melanoma and normal skin. / Saul, Louise; Ilieva, Kristina M; Bax, Heather J; Karagiannis, Panagiotis; Correa, Isabel; Rodriguez-Hernandez, Irene; Josephs, Debra H; Tosi, Isabella; Egbuniwe, Isioma U; Lombardi, Sara; Crescioli, Silvia; Hobbs, Carl; Villanova, Federica; Cheung, Anthony; Geh, Jenny L C; Healy, Ciaran; Harries, Mark; Sanz-Moreno, Victoria; Fear, David J; Spicer, James F; Lacy, Katie E; Nestle, Frank O; Karagiannis, Sophia N.

In: SCI REP-UK, Vol. 6, 14.07.2016, p. 29736.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Saul, L, Ilieva, KM, Bax, HJ, Karagiannis, P, Correa, I, Rodriguez-Hernandez, I, Josephs, DH, Tosi, I, Egbuniwe, IU, Lombardi, S, Crescioli, S, Hobbs, C, Villanova, F, Cheung, A, Geh, JLC, Healy, C, Harries, M, Sanz-Moreno, V, Fear, DJ, Spicer, JF, Lacy, KE, Nestle, FO & Karagiannis, SN 2016, 'IgG subclass switching and clonal expansion in cutaneous melanoma and normal skin', SCI REP-UK, vol. 6, pp. 29736. https://doi.org/10.1038/srep29736

APA

Saul, L., Ilieva, K. M., Bax, H. J., Karagiannis, P., Correa, I., Rodriguez-Hernandez, I., Josephs, D. H., Tosi, I., Egbuniwe, I. U., Lombardi, S., Crescioli, S., Hobbs, C., Villanova, F., Cheung, A., Geh, J. L. C., Healy, C., Harries, M., Sanz-Moreno, V., Fear, D. J., ... Karagiannis, S. N. (2016). IgG subclass switching and clonal expansion in cutaneous melanoma and normal skin. SCI REP-UK, 6, 29736. https://doi.org/10.1038/srep29736

Vancouver

Saul L, Ilieva KM, Bax HJ, Karagiannis P, Correa I, Rodriguez-Hernandez I et al. IgG subclass switching and clonal expansion in cutaneous melanoma and normal skin. SCI REP-UK. 2016 Jul 14;6:29736. https://doi.org/10.1038/srep29736

Bibtex

@article{11a9ac0ffe34453fb7c69a6a0d615812,
title = "IgG subclass switching and clonal expansion in cutaneous melanoma and normal skin",
abstract = "B cells participate in immune surveillance in human circulation and tissues, including tumors such as melanoma. By contrast, the role of humoral responses in cutaneous immunity is underappreciated. We report circulating skin-homing CD22+CLA+B cells in healthy volunteers and melanoma patients (n = 73) and CD22+ cells in melanoma and normal skin samples (n = 189). Normal and malignant skin featured mature IgG and CD22 mRNA, alongside mRNA for the transiently-expressed enzyme Activation-induced cytidine Deaminase (AID). Gene expression analyses of publically-available data (n = 234 GEO, n = 384 TCGA) confirmed heightened humoral responses (CD20, CD22, AID) in melanoma. Analyses of 51 melanoma-associated and 29 normal skin-derived IgG sequence repertoires revealed lower IgG1/IgGtotal representation compared with antibodies from circulating B cells. Consistent with AID, comparable somatic hypermutation frequencies and class-switching indicated affinity-matured antibodies in normal and malignant skin. A melanoma-associated antibody subset featured shorter complementarity-determining (CDR3) regions relative to those from circulating B cells. Clonal amplification in melanoma-associated antibodies and homology modeling indicated differential potential antigen recognition profiles between normal skin and melanoma sequences, suggesting distinct antibody repertoires. Evidence for IgG-expressing B cells, class switching and antibody maturation in normal and malignant skin and clonally-expanded antibodies in melanoma, support the involvement of mature B cells in cutaneous immunity.",
keywords = "Journal Article",
author = "Louise Saul and Ilieva, {Kristina M} and Bax, {Heather J} and Panagiotis Karagiannis and Isabel Correa and Irene Rodriguez-Hernandez and Josephs, {Debra H} and Isabella Tosi and Egbuniwe, {Isioma U} and Sara Lombardi and Silvia Crescioli and Carl Hobbs and Federica Villanova and Anthony Cheung and Geh, {Jenny L C} and Ciaran Healy and Mark Harries and Victoria Sanz-Moreno and Fear, {David J} and Spicer, {James F} and Lacy, {Katie E} and Nestle, {Frank O} and Karagiannis, {Sophia N}",
year = "2016",
month = jul,
day = "14",
doi = "10.1038/srep29736",
language = "English",
volume = "6",
pages = "29736",
journal = "SCI REP-UK",
issn = "2045-2322",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - IgG subclass switching and clonal expansion in cutaneous melanoma and normal skin

AU - Saul, Louise

AU - Ilieva, Kristina M

AU - Bax, Heather J

AU - Karagiannis, Panagiotis

AU - Correa, Isabel

AU - Rodriguez-Hernandez, Irene

AU - Josephs, Debra H

AU - Tosi, Isabella

AU - Egbuniwe, Isioma U

AU - Lombardi, Sara

AU - Crescioli, Silvia

AU - Hobbs, Carl

AU - Villanova, Federica

AU - Cheung, Anthony

AU - Geh, Jenny L C

AU - Healy, Ciaran

AU - Harries, Mark

AU - Sanz-Moreno, Victoria

AU - Fear, David J

AU - Spicer, James F

AU - Lacy, Katie E

AU - Nestle, Frank O

AU - Karagiannis, Sophia N

PY - 2016/7/14

Y1 - 2016/7/14

N2 - B cells participate in immune surveillance in human circulation and tissues, including tumors such as melanoma. By contrast, the role of humoral responses in cutaneous immunity is underappreciated. We report circulating skin-homing CD22+CLA+B cells in healthy volunteers and melanoma patients (n = 73) and CD22+ cells in melanoma and normal skin samples (n = 189). Normal and malignant skin featured mature IgG and CD22 mRNA, alongside mRNA for the transiently-expressed enzyme Activation-induced cytidine Deaminase (AID). Gene expression analyses of publically-available data (n = 234 GEO, n = 384 TCGA) confirmed heightened humoral responses (CD20, CD22, AID) in melanoma. Analyses of 51 melanoma-associated and 29 normal skin-derived IgG sequence repertoires revealed lower IgG1/IgGtotal representation compared with antibodies from circulating B cells. Consistent with AID, comparable somatic hypermutation frequencies and class-switching indicated affinity-matured antibodies in normal and malignant skin. A melanoma-associated antibody subset featured shorter complementarity-determining (CDR3) regions relative to those from circulating B cells. Clonal amplification in melanoma-associated antibodies and homology modeling indicated differential potential antigen recognition profiles between normal skin and melanoma sequences, suggesting distinct antibody repertoires. Evidence for IgG-expressing B cells, class switching and antibody maturation in normal and malignant skin and clonally-expanded antibodies in melanoma, support the involvement of mature B cells in cutaneous immunity.

AB - B cells participate in immune surveillance in human circulation and tissues, including tumors such as melanoma. By contrast, the role of humoral responses in cutaneous immunity is underappreciated. We report circulating skin-homing CD22+CLA+B cells in healthy volunteers and melanoma patients (n = 73) and CD22+ cells in melanoma and normal skin samples (n = 189). Normal and malignant skin featured mature IgG and CD22 mRNA, alongside mRNA for the transiently-expressed enzyme Activation-induced cytidine Deaminase (AID). Gene expression analyses of publically-available data (n = 234 GEO, n = 384 TCGA) confirmed heightened humoral responses (CD20, CD22, AID) in melanoma. Analyses of 51 melanoma-associated and 29 normal skin-derived IgG sequence repertoires revealed lower IgG1/IgGtotal representation compared with antibodies from circulating B cells. Consistent with AID, comparable somatic hypermutation frequencies and class-switching indicated affinity-matured antibodies in normal and malignant skin. A melanoma-associated antibody subset featured shorter complementarity-determining (CDR3) regions relative to those from circulating B cells. Clonal amplification in melanoma-associated antibodies and homology modeling indicated differential potential antigen recognition profiles between normal skin and melanoma sequences, suggesting distinct antibody repertoires. Evidence for IgG-expressing B cells, class switching and antibody maturation in normal and malignant skin and clonally-expanded antibodies in melanoma, support the involvement of mature B cells in cutaneous immunity.

KW - Journal Article

U2 - 10.1038/srep29736

DO - 10.1038/srep29736

M3 - SCORING: Journal article

C2 - 27411958

VL - 6

SP - 29736

JO - SCI REP-UK

JF - SCI REP-UK

SN - 2045-2322

ER -