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Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients.

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Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients. / Binder, Thomas; Siegert, W; Kruse, A; Oettle, H; Wilborn, F; Peng, R; Timm, H; Neuhaus, P; Schmidt, C A.

In: J VIROL METHODS, Vol. 78, No. 1-2, 1-2, 1999, p. 153-162.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Binder, T, Siegert, W, Kruse, A, Oettle, H, Wilborn, F, Peng, R, Timm, H, Neuhaus, P & Schmidt, CA 1999, 'Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients.', J VIROL METHODS, vol. 78, no. 1-2, 1-2, pp. 153-162. <http://www.ncbi.nlm.nih.gov/pubmed/10204705?dopt=Citation>

APA

Binder, T., Siegert, W., Kruse, A., Oettle, H., Wilborn, F., Peng, R., Timm, H., Neuhaus, P., & Schmidt, C. A. (1999). Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients. J VIROL METHODS, 78(1-2), 153-162. [1-2]. http://www.ncbi.nlm.nih.gov/pubmed/10204705?dopt=Citation

Vancouver

Binder T, Siegert W, Kruse A, Oettle H, Wilborn F, Peng R et al. Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients. J VIROL METHODS. 1999;78(1-2):153-162. 1-2.

Bibtex

@article{6c9edfa76f29493a934bf85a35b73bef,
title = "Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients.",
abstract = "Single strand conformation polymorphism analysis (SSCP) of PCR-amplified DNA and subsequent DNA sequencing of human cytomegalovirus (HCMV) glycoprotein B (gB) gene were applied to identify known HCMV strains and to detect new virus variants. 61 HCMV PCR positive patients were studied out of a cohort of 410 patients after liver transplantation (LTX). SSCP was able to distinguish between strains Davis, AD169, and Towne, and in addition could identify five new virus variants (Berlin B, C, E, F, and H). Their frequency, gB and gH types were determined. Simultaneous infections with two or three strains or variants, as well as a switch from one virus to another virus were observed during long-term follow-up. No correlation between the occurrence of certain virus strains or gB types and defined clinical manifestations of HCMV infection after LTX was drawn.",
author = "Thomas Binder and W Siegert and A Kruse and H Oettle and F Wilborn and R Peng and H Timm and P Neuhaus and Schmidt, {C A}",
year = "1999",
language = "Deutsch",
volume = "78",
pages = "153--162",
journal = "J VIROL METHODS",
issn = "0166-0934",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Identification of human cytomegalovirus variants by analysis of single strand conformation polymorphism and DNA sequencing of the envelope glycoprotein B gene region-distribution frequency in liver transplant recipients.

AU - Binder, Thomas

AU - Siegert, W

AU - Kruse, A

AU - Oettle, H

AU - Wilborn, F

AU - Peng, R

AU - Timm, H

AU - Neuhaus, P

AU - Schmidt, C A

PY - 1999

Y1 - 1999

N2 - Single strand conformation polymorphism analysis (SSCP) of PCR-amplified DNA and subsequent DNA sequencing of human cytomegalovirus (HCMV) glycoprotein B (gB) gene were applied to identify known HCMV strains and to detect new virus variants. 61 HCMV PCR positive patients were studied out of a cohort of 410 patients after liver transplantation (LTX). SSCP was able to distinguish between strains Davis, AD169, and Towne, and in addition could identify five new virus variants (Berlin B, C, E, F, and H). Their frequency, gB and gH types were determined. Simultaneous infections with two or three strains or variants, as well as a switch from one virus to another virus were observed during long-term follow-up. No correlation between the occurrence of certain virus strains or gB types and defined clinical manifestations of HCMV infection after LTX was drawn.

AB - Single strand conformation polymorphism analysis (SSCP) of PCR-amplified DNA and subsequent DNA sequencing of human cytomegalovirus (HCMV) glycoprotein B (gB) gene were applied to identify known HCMV strains and to detect new virus variants. 61 HCMV PCR positive patients were studied out of a cohort of 410 patients after liver transplantation (LTX). SSCP was able to distinguish between strains Davis, AD169, and Towne, and in addition could identify five new virus variants (Berlin B, C, E, F, and H). Their frequency, gB and gH types were determined. Simultaneous infections with two or three strains or variants, as well as a switch from one virus to another virus were observed during long-term follow-up. No correlation between the occurrence of certain virus strains or gB types and defined clinical manifestations of HCMV infection after LTX was drawn.

M3 - SCORING: Zeitschriftenaufsatz

VL - 78

SP - 153

EP - 162

JO - J VIROL METHODS

JF - J VIROL METHODS

SN - 0166-0934

IS - 1-2

M1 - 1-2

ER -