Identification of antigenic proteins of a Theileria species pathogenic for small ruminants in China recognized by antisera of infected animals.
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Identification of antigenic proteins of a Theileria species pathogenic for small ruminants in China recognized by antisera of infected animals. / Miranda, Joana; Stumme, Barbara; Beyer, Doreen; Cruz, Helder; Oliva, Abel González; Bakheit, Mohammed; Wicklein, Daniel; Yin, Hong; Lou, Jianxun; Ahmed, Jabbar S; Seitzer, Ulrike.
In: ANN NY ACAD SCI, Vol. 1026, 2004, p. 161-164.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Identification of antigenic proteins of a Theileria species pathogenic for small ruminants in China recognized by antisera of infected animals.
AU - Miranda, Joana
AU - Stumme, Barbara
AU - Beyer, Doreen
AU - Cruz, Helder
AU - Oliva, Abel González
AU - Bakheit, Mohammed
AU - Wicklein, Daniel
AU - Yin, Hong
AU - Lou, Jianxun
AU - Ahmed, Jabbar S
AU - Seitzer, Ulrike
PY - 2004
Y1 - 2004
N2 - The antigenic proteins of the piroplasm stage of Theileria species (China), the causative agent of theilerosis of small ruminants in China, were analyzed by Western blot, revealing several specific immunoreactive proteins of different predicted molecular weights. Furthermore, sera from Theileria species (China)-infected animals were probed for reactivity with the TaSP protein of T. annulata, for which a homologue has been described in Theileria species (China). Affinity chromatography demonstrated the presence of TaSP-reactive antibodies, and the majority of the sera showed reactivity with this protein both in Western blots and in ELISA. The identified parasite antigens and TaSP will be assessed for their suitability for developing diagnostic methods as well as evaluated for their capacity to stimulated host immune competent cells.
AB - The antigenic proteins of the piroplasm stage of Theileria species (China), the causative agent of theilerosis of small ruminants in China, were analyzed by Western blot, revealing several specific immunoreactive proteins of different predicted molecular weights. Furthermore, sera from Theileria species (China)-infected animals were probed for reactivity with the TaSP protein of T. annulata, for which a homologue has been described in Theileria species (China). Affinity chromatography demonstrated the presence of TaSP-reactive antibodies, and the majority of the sera showed reactivity with this protein both in Western blots and in ELISA. The identified parasite antigens and TaSP will be assessed for their suitability for developing diagnostic methods as well as evaluated for their capacity to stimulated host immune competent cells.
KW - Animals
KW - China
KW - Enzyme-Linked Immunosorbent Assay
KW - Blotting, Western
KW - Molecular Weight
KW - Cross Reactions
KW - Antigens, Protozoan/analysis
KW - Chromatography, Affinity
KW - Protozoan Proteins/analysis/immunology
KW - Ruminants
KW - Theileria/immunology/pathogenicity
KW - Theileriasis/immunology/pathology
KW - Animals
KW - China
KW - Enzyme-Linked Immunosorbent Assay
KW - Blotting, Western
KW - Molecular Weight
KW - Cross Reactions
KW - Antigens, Protozoan/analysis
KW - Chromatography, Affinity
KW - Protozoan Proteins/analysis/immunology
KW - Ruminants
KW - Theileria/immunology/pathogenicity
KW - Theileriasis/immunology/pathology
M3 - SCORING: Journal article
VL - 1026
SP - 161
EP - 164
JO - ANN NY ACAD SCI
JF - ANN NY ACAD SCI
SN - 0077-8923
ER -
