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Identification of antibodies against extracellular matrix proteins in human osteoarthritis

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Identification of antibodies against extracellular matrix proteins in human osteoarthritis. / Ruthard, Johannes; Hermes, Gabriele; Hartmann, Ursula; Sengle, Gerhard; Pongratz, Georg; Ostendorf, Benedikt; Schneider, Matthias; Höllriegl, Stefan; Zaucke, Frank; Wagener, Raimund; Streichert, Thomas; Klatt, Andreas R.

In: BIOCHEM BIOPH RES CO, Vol. 503, No. 3, 10.09.2018, p. 1273-1277.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ruthard, J, Hermes, G, Hartmann, U, Sengle, G, Pongratz, G, Ostendorf, B, Schneider, M, Höllriegl, S, Zaucke, F, Wagener, R, Streichert, T & Klatt, AR 2018, 'Identification of antibodies against extracellular matrix proteins in human osteoarthritis', BIOCHEM BIOPH RES CO, vol. 503, no. 3, pp. 1273-1277. https://doi.org/10.1016/j.bbrc.2018.07.036

APA

Ruthard, J., Hermes, G., Hartmann, U., Sengle, G., Pongratz, G., Ostendorf, B., Schneider, M., Höllriegl, S., Zaucke, F., Wagener, R., Streichert, T., & Klatt, A. R. (2018). Identification of antibodies against extracellular matrix proteins in human osteoarthritis. BIOCHEM BIOPH RES CO, 503(3), 1273-1277. https://doi.org/10.1016/j.bbrc.2018.07.036

Vancouver

Ruthard J, Hermes G, Hartmann U, Sengle G, Pongratz G, Ostendorf B et al. Identification of antibodies against extracellular matrix proteins in human osteoarthritis. BIOCHEM BIOPH RES CO. 2018 Sep 10;503(3):1273-1277. https://doi.org/10.1016/j.bbrc.2018.07.036

Bibtex

@article{2aca7fae4fdd45429a7be820aba74de6,
title = "Identification of antibodies against extracellular matrix proteins in human osteoarthritis",
abstract = "We investigated the presence of autoantibodies against the extracellular matrix proteins thrombospondin-4 (TSP-4), cartilage oligomeric matrix protein (COMP), C-type lectin domain family 3 member A (CLEC3A), collagen II, collagen VI, matrilin-3, and fibrillin-2 in the serum of osteoarthritis (OA) patients. We compared those results with the presence of such antibodies in rheumatoid arthritis (RA) patients and in healthy donors (HD). Our study examines whether antibodies against extracellular proteins can be used as potential biomarkers to support the clinical diagnosis of OA. 10 OA, 10 RA patients and 10 HD were enrolled in this explorative cross-sectional study. SDS-PAGE and immunoblot were used to investigate the presence of antibodies against extracellular matrix proteins. The serum of 5/10 OA patients but 0/10 HD exhibited TSP-4 IgG isotype antibodies (P = 0.033). The serum of 8/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4 or COMP (P = 0.005). The serum of 9/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4, COMP or CLEC3A (P = 0.005). We found strong evidence for the presence of IgG isotype autoantibodies against the cartilage extracellular matrix proteins TSP-4, COMP and CLEC3A in OA. The detection of IgG isotype autoantibodies against TSP-4, COMP and CLEC3A may support the clinical diagnosis of OA. OA with autoantibodies against cartilage extracellular matrix proteins defines a new OA subgroup suggesting that patients with high concentrations of autoantibodies may benefit from an immune suppressive therapy.",
keywords = "Arthritis, Rheumatoid, Autoantibodies, Biomarkers, Cartilage Oligomeric Matrix Protein, Collagen Type II, Collagen Type VI, Fibrillin-2, Humans, Lectins, C-Type, Matrilin Proteins, Middle Aged, Osteoarthritis, Thrombospondins, Journal Article, Research Support, Non-U.S. Gov't",
author = "Johannes Ruthard and Gabriele Hermes and Ursula Hartmann and Gerhard Sengle and Georg Pongratz and Benedikt Ostendorf and Matthias Schneider and Stefan H{\"o}llriegl and Frank Zaucke and Raimund Wagener and Thomas Streichert and Klatt, {Andreas R}",
note = "Copyright {\textcopyright} 2018 Elsevier Inc. All rights reserved.",
year = "2018",
month = sep,
day = "10",
doi = "10.1016/j.bbrc.2018.07.036",
language = "English",
volume = "503",
pages = "1273--1277",
journal = "BIOCHEM BIOPH RES CO",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Identification of antibodies against extracellular matrix proteins in human osteoarthritis

AU - Ruthard, Johannes

AU - Hermes, Gabriele

AU - Hartmann, Ursula

AU - Sengle, Gerhard

AU - Pongratz, Georg

AU - Ostendorf, Benedikt

AU - Schneider, Matthias

AU - Höllriegl, Stefan

AU - Zaucke, Frank

AU - Wagener, Raimund

AU - Streichert, Thomas

AU - Klatt, Andreas R

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2018/9/10

Y1 - 2018/9/10

N2 - We investigated the presence of autoantibodies against the extracellular matrix proteins thrombospondin-4 (TSP-4), cartilage oligomeric matrix protein (COMP), C-type lectin domain family 3 member A (CLEC3A), collagen II, collagen VI, matrilin-3, and fibrillin-2 in the serum of osteoarthritis (OA) patients. We compared those results with the presence of such antibodies in rheumatoid arthritis (RA) patients and in healthy donors (HD). Our study examines whether antibodies against extracellular proteins can be used as potential biomarkers to support the clinical diagnosis of OA. 10 OA, 10 RA patients and 10 HD were enrolled in this explorative cross-sectional study. SDS-PAGE and immunoblot were used to investigate the presence of antibodies against extracellular matrix proteins. The serum of 5/10 OA patients but 0/10 HD exhibited TSP-4 IgG isotype antibodies (P = 0.033). The serum of 8/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4 or COMP (P = 0.005). The serum of 9/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4, COMP or CLEC3A (P = 0.005). We found strong evidence for the presence of IgG isotype autoantibodies against the cartilage extracellular matrix proteins TSP-4, COMP and CLEC3A in OA. The detection of IgG isotype autoantibodies against TSP-4, COMP and CLEC3A may support the clinical diagnosis of OA. OA with autoantibodies against cartilage extracellular matrix proteins defines a new OA subgroup suggesting that patients with high concentrations of autoantibodies may benefit from an immune suppressive therapy.

AB - We investigated the presence of autoantibodies against the extracellular matrix proteins thrombospondin-4 (TSP-4), cartilage oligomeric matrix protein (COMP), C-type lectin domain family 3 member A (CLEC3A), collagen II, collagen VI, matrilin-3, and fibrillin-2 in the serum of osteoarthritis (OA) patients. We compared those results with the presence of such antibodies in rheumatoid arthritis (RA) patients and in healthy donors (HD). Our study examines whether antibodies against extracellular proteins can be used as potential biomarkers to support the clinical diagnosis of OA. 10 OA, 10 RA patients and 10 HD were enrolled in this explorative cross-sectional study. SDS-PAGE and immunoblot were used to investigate the presence of antibodies against extracellular matrix proteins. The serum of 5/10 OA patients but 0/10 HD exhibited TSP-4 IgG isotype antibodies (P = 0.033). The serum of 8/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4 or COMP (P = 0.005). The serum of 9/10 OA patients but only 1/10 HD exhibited IgG isotype antibodies against TSP-4, COMP or CLEC3A (P = 0.005). We found strong evidence for the presence of IgG isotype autoantibodies against the cartilage extracellular matrix proteins TSP-4, COMP and CLEC3A in OA. The detection of IgG isotype autoantibodies against TSP-4, COMP and CLEC3A may support the clinical diagnosis of OA. OA with autoantibodies against cartilage extracellular matrix proteins defines a new OA subgroup suggesting that patients with high concentrations of autoantibodies may benefit from an immune suppressive therapy.

KW - Arthritis, Rheumatoid

KW - Autoantibodies

KW - Biomarkers

KW - Cartilage Oligomeric Matrix Protein

KW - Collagen Type II

KW - Collagen Type VI

KW - Fibrillin-2

KW - Humans

KW - Lectins, C-Type

KW - Matrilin Proteins

KW - Middle Aged

KW - Osteoarthritis

KW - Thrombospondins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.bbrc.2018.07.036

DO - 10.1016/j.bbrc.2018.07.036

M3 - SCORING: Journal article

C2 - 30001809

VL - 503

SP - 1273

EP - 1277

JO - BIOCHEM BIOPH RES CO

JF - BIOCHEM BIOPH RES CO

SN - 0006-291X

IS - 3

ER -