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Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay

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Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay. / Baccelli, Irène; Schneeweiss, Andreas; Riethdorf, Sabine; Stenzinger, Albrecht; Schillert, Anja; Vogel, Vanessa; Klein, Corinna; Saini, Massimo; Bäuerle, Tobias; Wallwiener, Markus; Holland-Letz, Tim; Höfner, Thomas; Sprick, Martin; Scharpff, Martina; Marmé, Frederik; Sinn, Hans Peter; Pantel, Klaus; Weichert, Wilko; Trumpp, Andreas.

In: NAT BIOTECHNOL, Vol. 31, No. 6, 01.06.2013, p. 539-44.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Baccelli, I, Schneeweiss, A, Riethdorf, S, Stenzinger, A, Schillert, A, Vogel, V, Klein, C, Saini, M, Bäuerle, T, Wallwiener, M, Holland-Letz, T, Höfner, T, Sprick, M, Scharpff, M, Marmé, F, Sinn, HP, Pantel, K, Weichert, W & Trumpp, A 2013, 'Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay', NAT BIOTECHNOL, vol. 31, no. 6, pp. 539-44. https://doi.org/10.1038/nbt.2576

APA

Baccelli, I., Schneeweiss, A., Riethdorf, S., Stenzinger, A., Schillert, A., Vogel, V., Klein, C., Saini, M., Bäuerle, T., Wallwiener, M., Holland-Letz, T., Höfner, T., Sprick, M., Scharpff, M., Marmé, F., Sinn, H. P., Pantel, K., Weichert, W., & Trumpp, A. (2013). Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay. NAT BIOTECHNOL, 31(6), 539-44. https://doi.org/10.1038/nbt.2576

Vancouver

Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V et al. Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay. NAT BIOTECHNOL. 2013 Jun 1;31(6):539-44. https://doi.org/10.1038/nbt.2576

Bibtex

@article{1fc17db20bcc4141b5432e6e6334370d,
title = "Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay",
abstract = "It has been hypothesized that carcinoma metastasis is initiated by a subpopulation of circulating tumor cells (CTCs) found in the blood of patients. However, although the presence of CTCs is an indicator of poor prognosis in several carcinoma entities, the existence and phenotype of metastasis-initiating cells (MICs) among CTCs has not been experimentally demonstrated. Here we developed a xenograft assay and used it to show that primary human luminal breast cancer CTCs contain MICs that give rise to bone, lung and liver metastases in mice. These MIC-containing CTC populations expressed EPCAM, CD44, CD47 and MET. In a small cohort of patients with metastases, the number of EPCAM(+)CD44(+)CD47(+)MET(+) CTCs, but not of bulk EPCAM(+) CTCs, correlated with lower overall survival and increased number of metastasic sites. These data describe functional circulating MICs and associated markers, which may aid the design of better tools to diagnose and treat metastatic breast cancer.",
keywords = "Adult, Aged, Animals, Breast Neoplasms, Cell Count, Cell Line, Tumor, Female, Gene Expression Regulation, Neoplastic, Humans, Mice, Middle Aged, Neoplasm Metastasis, Neoplastic Cells, Circulating, Prognosis, Tumor Markers, Biological, Xenograft Model Antitumor Assays",
author = "Ir{\`e}ne Baccelli and Andreas Schneeweiss and Sabine Riethdorf and Albrecht Stenzinger and Anja Schillert and Vanessa Vogel and Corinna Klein and Massimo Saini and Tobias B{\"a}uerle and Markus Wallwiener and Tim Holland-Letz and Thomas H{\"o}fner and Martin Sprick and Martina Scharpff and Frederik Marm{\'e} and Sinn, {Hans Peter} and Klaus Pantel and Wilko Weichert and Andreas Trumpp",
year = "2013",
month = jun,
day = "1",
doi = "10.1038/nbt.2576",
language = "English",
volume = "31",
pages = "539--44",
journal = "NAT BIOTECHNOL",
issn = "1087-0156",
publisher = "NATURE PUBLISHING GROUP",
number = "6",

}

RIS

TY - JOUR

T1 - Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay

AU - Baccelli, Irène

AU - Schneeweiss, Andreas

AU - Riethdorf, Sabine

AU - Stenzinger, Albrecht

AU - Schillert, Anja

AU - Vogel, Vanessa

AU - Klein, Corinna

AU - Saini, Massimo

AU - Bäuerle, Tobias

AU - Wallwiener, Markus

AU - Holland-Letz, Tim

AU - Höfner, Thomas

AU - Sprick, Martin

AU - Scharpff, Martina

AU - Marmé, Frederik

AU - Sinn, Hans Peter

AU - Pantel, Klaus

AU - Weichert, Wilko

AU - Trumpp, Andreas

PY - 2013/6/1

Y1 - 2013/6/1

N2 - It has been hypothesized that carcinoma metastasis is initiated by a subpopulation of circulating tumor cells (CTCs) found in the blood of patients. However, although the presence of CTCs is an indicator of poor prognosis in several carcinoma entities, the existence and phenotype of metastasis-initiating cells (MICs) among CTCs has not been experimentally demonstrated. Here we developed a xenograft assay and used it to show that primary human luminal breast cancer CTCs contain MICs that give rise to bone, lung and liver metastases in mice. These MIC-containing CTC populations expressed EPCAM, CD44, CD47 and MET. In a small cohort of patients with metastases, the number of EPCAM(+)CD44(+)CD47(+)MET(+) CTCs, but not of bulk EPCAM(+) CTCs, correlated with lower overall survival and increased number of metastasic sites. These data describe functional circulating MICs and associated markers, which may aid the design of better tools to diagnose and treat metastatic breast cancer.

AB - It has been hypothesized that carcinoma metastasis is initiated by a subpopulation of circulating tumor cells (CTCs) found in the blood of patients. However, although the presence of CTCs is an indicator of poor prognosis in several carcinoma entities, the existence and phenotype of metastasis-initiating cells (MICs) among CTCs has not been experimentally demonstrated. Here we developed a xenograft assay and used it to show that primary human luminal breast cancer CTCs contain MICs that give rise to bone, lung and liver metastases in mice. These MIC-containing CTC populations expressed EPCAM, CD44, CD47 and MET. In a small cohort of patients with metastases, the number of EPCAM(+)CD44(+)CD47(+)MET(+) CTCs, but not of bulk EPCAM(+) CTCs, correlated with lower overall survival and increased number of metastasic sites. These data describe functional circulating MICs and associated markers, which may aid the design of better tools to diagnose and treat metastatic breast cancer.

KW - Adult

KW - Aged

KW - Animals

KW - Breast Neoplasms

KW - Cell Count

KW - Cell Line, Tumor

KW - Female

KW - Gene Expression Regulation, Neoplastic

KW - Humans

KW - Mice

KW - Middle Aged

KW - Neoplasm Metastasis

KW - Neoplastic Cells, Circulating

KW - Prognosis

KW - Tumor Markers, Biological

KW - Xenograft Model Antitumor Assays

U2 - 10.1038/nbt.2576

DO - 10.1038/nbt.2576

M3 - SCORING: Journal article

C2 - 23609047

VL - 31

SP - 539

EP - 544

JO - NAT BIOTECHNOL

JF - NAT BIOTECHNOL

SN - 1087-0156

IS - 6

ER -