Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes

Anne Hofmann; Stefanie Wienkoop; Sönke Harder; Fabian Bartlog; Sabine Lüthje

doi:10.3390/ijms21228872

Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes

Standard

Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes. / Hofmann, Anne; Wienkoop, Stefanie; Harder, Sönke; Bartlog, Fabian; Lüthje, Sabine.

In: INT J MOL SCI, Vol. 21, No. 22, 8872, 23.11.2020.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hofmann, A, Wienkoop, S, Harder, S, Bartlog, F & Lüthje, S 2020, 'Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes', INT J MOL SCI, vol. 21, no. 22, 8872. https://doi.org/10.3390/ijms21228872

APA

Hofmann, A., Wienkoop, S., Harder, S., Bartlog, F., & Lüthje, S. (2020). Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes. INT J MOL SCI, 21(22), [8872]. https://doi.org/10.3390/ijms21228872

Vancouver

Hofmann A, Wienkoop S, Harder S, Bartlog F, Lüthje S. Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes. INT J MOL SCI. 2020 Nov 23;21(22). 8872. https://doi.org/10.3390/ijms21228872

Bibtex

@article{07f0be8b90a34ec8b6f58476934b62f9,

title = "Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes",

abstract = "Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production and scavenging enzymes in plants. The influence of hypoxia on roots of hydroponically grown maize (Zea mays L.) plants was investigated. Gene expression (RNA Seq and RT-qPCR) and proteome (LC-MS/MS and 2D-PAGE) analyses were used to determine the alterations in soluble and membrane-bound class III peroxidases under hypoxia. Gel-free peroxidase analyses of plasma membrane-bound proteins showed an increased abundance of ZmPrx03, ZmPrx24, ZmPrx81, and ZmPr85 in stressed samples. Furthermore, RT-qPCR analyses of the corresponding peroxidase genes revealed an increased expression. These peroxidases could be separated with 2D-PAGE and identified by mass spectrometry. An increased abundance of ZmPrx03 and ZmPrx85 was determined. Further peroxidases were identified in detergent-insoluble membranes. Co-regulation with a respiratory burst oxidase homolog (Rboh) and key enzymes of the phenylpropanoid pathway indicates a function of the peroxidases in membrane protection, aerenchyma formation, and cell wall remodeling under hypoxia. This hypothesis was supported by the following: (i) an elevated level of hydrogen peroxide and aerenchyma formation; (ii) an increased guaiacol peroxidase activity in membrane fractions of stressed samples, whereas a decrease was observed in soluble fractions; and (iii) alterations in lignified cells, cellulose, and suberin in root cross-sections.",

keywords = "Cell Hypoxia/genetics, Cell Membrane/genetics, Cell Wall/genetics, Chromatography, Liquid, Gene Expression Regulation, Plant, Isoenzymes/genetics, NADPH Oxidases/genetics, Oxidation-Reduction, Peroxidase/genetics, Peroxidases/genetics, Plant Roots/enzymology, Protein Binding/genetics, Proteome/genetics, Reactive Oxygen Species/metabolism, Tandem Mass Spectrometry, Zea mays/enzymology",

author = "Anne Hofmann and Stefanie Wienkoop and S{\"o}nke Harder and Fabian Bartlog and Sabine L{\"u}thje",

year = "2020",

month = nov,

day = "23",

doi = "10.3390/ijms21228872",

language = "English",

volume = "21",

journal = "INT J MOL SCI",

issn = "1661-6596",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "22",

}

RIS

TY - JOUR

T1 - Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes

AU - Hofmann, Anne

AU - Wienkoop, Stefanie

AU - Harder, Sönke

AU - Bartlog, Fabian

AU - Lüthje, Sabine

PY - 2020/11/23

Y1 - 2020/11/23

N2 - Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production and scavenging enzymes in plants. The influence of hypoxia on roots of hydroponically grown maize (Zea mays L.) plants was investigated. Gene expression (RNA Seq and RT-qPCR) and proteome (LC-MS/MS and 2D-PAGE) analyses were used to determine the alterations in soluble and membrane-bound class III peroxidases under hypoxia. Gel-free peroxidase analyses of plasma membrane-bound proteins showed an increased abundance of ZmPrx03, ZmPrx24, ZmPrx81, and ZmPr85 in stressed samples. Furthermore, RT-qPCR analyses of the corresponding peroxidase genes revealed an increased expression. These peroxidases could be separated with 2D-PAGE and identified by mass spectrometry. An increased abundance of ZmPrx03 and ZmPrx85 was determined. Further peroxidases were identified in detergent-insoluble membranes. Co-regulation with a respiratory burst oxidase homolog (Rboh) and key enzymes of the phenylpropanoid pathway indicates a function of the peroxidases in membrane protection, aerenchyma formation, and cell wall remodeling under hypoxia. This hypothesis was supported by the following: (i) an elevated level of hydrogen peroxide and aerenchyma formation; (ii) an increased guaiacol peroxidase activity in membrane fractions of stressed samples, whereas a decrease was observed in soluble fractions; and (iii) alterations in lignified cells, cellulose, and suberin in root cross-sections.

AB - Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production and scavenging enzymes in plants. The influence of hypoxia on roots of hydroponically grown maize (Zea mays L.) plants was investigated. Gene expression (RNA Seq and RT-qPCR) and proteome (LC-MS/MS and 2D-PAGE) analyses were used to determine the alterations in soluble and membrane-bound class III peroxidases under hypoxia. Gel-free peroxidase analyses of plasma membrane-bound proteins showed an increased abundance of ZmPrx03, ZmPrx24, ZmPrx81, and ZmPr85 in stressed samples. Furthermore, RT-qPCR analyses of the corresponding peroxidase genes revealed an increased expression. These peroxidases could be separated with 2D-PAGE and identified by mass spectrometry. An increased abundance of ZmPrx03 and ZmPrx85 was determined. Further peroxidases were identified in detergent-insoluble membranes. Co-regulation with a respiratory burst oxidase homolog (Rboh) and key enzymes of the phenylpropanoid pathway indicates a function of the peroxidases in membrane protection, aerenchyma formation, and cell wall remodeling under hypoxia. This hypothesis was supported by the following: (i) an elevated level of hydrogen peroxide and aerenchyma formation; (ii) an increased guaiacol peroxidase activity in membrane fractions of stressed samples, whereas a decrease was observed in soluble fractions; and (iii) alterations in lignified cells, cellulose, and suberin in root cross-sections.

KW - Cell Hypoxia/genetics

KW - Cell Membrane/genetics

KW - Cell Wall/genetics

KW - Chromatography, Liquid

KW - Gene Expression Regulation, Plant

KW - Isoenzymes/genetics

KW - NADPH Oxidases/genetics

KW - Oxidation-Reduction

KW - Peroxidase/genetics

KW - Peroxidases/genetics

KW - Plant Roots/enzymology

KW - Protein Binding/genetics

KW - Proteome/genetics

KW - Reactive Oxygen Species/metabolism

KW - Tandem Mass Spectrometry

KW - Zea mays/enzymology

U2 - 10.3390/ijms21228872

DO - 10.3390/ijms21228872

M3 - SCORING: Journal article

C2 - 33238617

VL - 21

JO - INT J MOL SCI

JF - INT J MOL SCI

SN - 1661-6596

IS - 22

M1 - 8872

ER -