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Hydroxyethyl starch normalizes platelet and leukocyte adhesion within pulmonary microcirculation during LPS-induced endotoxemia

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Hydroxyethyl starch normalizes platelet and leukocyte adhesion within pulmonary microcirculation during LPS-induced endotoxemia. / Küpper, Sebastian; Mees, Soeren Torge; Gassmann, Peter; Brodde, Martin F; Kehrel, Beate; Haier, Joerg.

In: SHOCK, Vol. 28, No. 3, 09.2007, p. 300-8.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Küpper, S, Mees, ST, Gassmann, P, Brodde, MF, Kehrel, B & Haier, J 2007, 'Hydroxyethyl starch normalizes platelet and leukocyte adhesion within pulmonary microcirculation during LPS-induced endotoxemia', SHOCK, vol. 28, no. 3, pp. 300-8. https://doi.org/10.1097/shk.0b013e3180340664

APA

Küpper, S., Mees, S. T., Gassmann, P., Brodde, M. F., Kehrel, B., & Haier, J. (2007). Hydroxyethyl starch normalizes platelet and leukocyte adhesion within pulmonary microcirculation during LPS-induced endotoxemia. SHOCK, 28(3), 300-8. https://doi.org/10.1097/shk.0b013e3180340664

Vancouver

Küpper S, Mees ST, Gassmann P, Brodde MF, Kehrel B, Haier J. Hydroxyethyl starch normalizes platelet and leukocyte adhesion within pulmonary microcirculation during LPS-induced endotoxemia. SHOCK. 2007 Sep;28(3):300-8. https://doi.org/10.1097/shk.0b013e3180340664

Bibtex

@article{4eb13403c72f4a2bbf8e4faf51072cf8,
title = "Hydroxyethyl starch normalizes platelet and leukocyte adhesion within pulmonary microcirculation during LPS-induced endotoxemia",
abstract = "Growing evidence supports substantial pathophysiological impact of platelets and their interactions on the development of septic lung failure. We developed a rat model of endotoxemia for direct in situ visualization of pulmonary microcirculation by in vivo fluorescence videomicroscopy. Male Sprague-Dawley rats were assigned to control, endotoxemia (Escherichia coli LPS, 15 mg/kg, i.v.), and fluid management for treatment of LPS-induced hypovolemia (Ringer lactate, hydroxyethyl starch [HES] 6%) groups (n = 7 each). Leukocytes were labeled in vivo by rhodamine, and 5 x 10(6) Calcein-AM-labeled nonactivated platelets were injected. Microcirculatory parameters (vessel diameter, ventilation-perfusion ratio) and adhesive characteristics of platelets and leukocytes (velocity, rolling, sticking) within the pulmonary microcirculation were quantified after endotoxin application under various regimens of fluid substitution for 60 min. A reduction of cell velocity and enhanced cell adhesion was seen in leukocytes and platelets (P < 0.05) after LPS injection. Fluid treatment with HES 6% resulted in a significant increase of platelet's velocity compared with the LPS group (442.86 +/- 20.60 vs. 343.93 +/- 11.17; P < 0.05), whereas Ringer lactate showed no beneficial effects. Similarly, HES 6% normalized LPS-induced platelet rolling and sticking as well as alterations in ventilation-perfusion ratio. Using direct visualization of the pulmonary microcirculation, we observed that platelet and leukocyte interactions are enhanced in the lung during LPS endotoxemia. Fluid therapy with HES 6% seems to have restorative effects on these cellular functions within the pulmonary microcirculation.",
keywords = "Animals, Blood Platelets, Blood Pressure, Cell Adhesion, Endotoxemia, Heart Rate, Hydroxyethyl Starch Derivatives, Leukocytes, Lipopolysaccharides, Lung, Male, Microcirculation, P-Selectin, Platelet Activation, Rats, Rats, Sprague-Dawley",
author = "Sebastian K{\"u}pper and Mees, {Soeren Torge} and Peter Gassmann and Brodde, {Martin F} and Beate Kehrel and Joerg Haier",
year = "2007",
month = sep,
doi = "10.1097/shk.0b013e3180340664",
language = "English",
volume = "28",
pages = "300--8",
journal = "SHOCK",
issn = "1073-2322",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

RIS

TY - JOUR

T1 - Hydroxyethyl starch normalizes platelet and leukocyte adhesion within pulmonary microcirculation during LPS-induced endotoxemia

AU - Küpper, Sebastian

AU - Mees, Soeren Torge

AU - Gassmann, Peter

AU - Brodde, Martin F

AU - Kehrel, Beate

AU - Haier, Joerg

PY - 2007/9

Y1 - 2007/9

N2 - Growing evidence supports substantial pathophysiological impact of platelets and their interactions on the development of septic lung failure. We developed a rat model of endotoxemia for direct in situ visualization of pulmonary microcirculation by in vivo fluorescence videomicroscopy. Male Sprague-Dawley rats were assigned to control, endotoxemia (Escherichia coli LPS, 15 mg/kg, i.v.), and fluid management for treatment of LPS-induced hypovolemia (Ringer lactate, hydroxyethyl starch [HES] 6%) groups (n = 7 each). Leukocytes were labeled in vivo by rhodamine, and 5 x 10(6) Calcein-AM-labeled nonactivated platelets were injected. Microcirculatory parameters (vessel diameter, ventilation-perfusion ratio) and adhesive characteristics of platelets and leukocytes (velocity, rolling, sticking) within the pulmonary microcirculation were quantified after endotoxin application under various regimens of fluid substitution for 60 min. A reduction of cell velocity and enhanced cell adhesion was seen in leukocytes and platelets (P < 0.05) after LPS injection. Fluid treatment with HES 6% resulted in a significant increase of platelet's velocity compared with the LPS group (442.86 +/- 20.60 vs. 343.93 +/- 11.17; P < 0.05), whereas Ringer lactate showed no beneficial effects. Similarly, HES 6% normalized LPS-induced platelet rolling and sticking as well as alterations in ventilation-perfusion ratio. Using direct visualization of the pulmonary microcirculation, we observed that platelet and leukocyte interactions are enhanced in the lung during LPS endotoxemia. Fluid therapy with HES 6% seems to have restorative effects on these cellular functions within the pulmonary microcirculation.

AB - Growing evidence supports substantial pathophysiological impact of platelets and their interactions on the development of septic lung failure. We developed a rat model of endotoxemia for direct in situ visualization of pulmonary microcirculation by in vivo fluorescence videomicroscopy. Male Sprague-Dawley rats were assigned to control, endotoxemia (Escherichia coli LPS, 15 mg/kg, i.v.), and fluid management for treatment of LPS-induced hypovolemia (Ringer lactate, hydroxyethyl starch [HES] 6%) groups (n = 7 each). Leukocytes were labeled in vivo by rhodamine, and 5 x 10(6) Calcein-AM-labeled nonactivated platelets were injected. Microcirculatory parameters (vessel diameter, ventilation-perfusion ratio) and adhesive characteristics of platelets and leukocytes (velocity, rolling, sticking) within the pulmonary microcirculation were quantified after endotoxin application under various regimens of fluid substitution for 60 min. A reduction of cell velocity and enhanced cell adhesion was seen in leukocytes and platelets (P < 0.05) after LPS injection. Fluid treatment with HES 6% resulted in a significant increase of platelet's velocity compared with the LPS group (442.86 +/- 20.60 vs. 343.93 +/- 11.17; P < 0.05), whereas Ringer lactate showed no beneficial effects. Similarly, HES 6% normalized LPS-induced platelet rolling and sticking as well as alterations in ventilation-perfusion ratio. Using direct visualization of the pulmonary microcirculation, we observed that platelet and leukocyte interactions are enhanced in the lung during LPS endotoxemia. Fluid therapy with HES 6% seems to have restorative effects on these cellular functions within the pulmonary microcirculation.

KW - Animals

KW - Blood Platelets

KW - Blood Pressure

KW - Cell Adhesion

KW - Endotoxemia

KW - Heart Rate

KW - Hydroxyethyl Starch Derivatives

KW - Leukocytes

KW - Lipopolysaccharides

KW - Lung

KW - Male

KW - Microcirculation

KW - P-Selectin

KW - Platelet Activation

KW - Rats

KW - Rats, Sprague-Dawley

U2 - 10.1097/shk.0b013e3180340664

DO - 10.1097/shk.0b013e3180340664

M3 - SCORING: Journal article

C2 - 17545948

VL - 28

SP - 300

EP - 308

JO - SHOCK

JF - SHOCK

SN - 1073-2322

IS - 3

ER -