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Hyaluronan export through plasma membranes depends on concurrent K+ efflux by K(ir) channels.

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Hyaluronan export through plasma membranes depends on concurrent K+ efflux by K(ir) channels. / Hagenfeld, Daniel; Borkenhagen, Beatrice; Schulz, Tobias; Schillers, Hermann; Schumacher, Udo; Prehm, Peter.

In: PLOS ONE, Vol. 7, No. 6, 6, 2012, p. 39096.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hagenfeld, D, Borkenhagen, B, Schulz, T, Schillers, H, Schumacher, U & Prehm, P 2012, 'Hyaluronan export through plasma membranes depends on concurrent K+ efflux by K(ir) channels.', PLOS ONE, vol. 7, no. 6, 6, pp. 39096. https://doi.org/10.1371/journal.pone.0039096

APA

Hagenfeld, D., Borkenhagen, B., Schulz, T., Schillers, H., Schumacher, U., & Prehm, P. (2012). Hyaluronan export through plasma membranes depends on concurrent K+ efflux by K(ir) channels. PLOS ONE, 7(6), 39096. [6]. https://doi.org/10.1371/journal.pone.0039096

Vancouver

Hagenfeld D, Borkenhagen B, Schulz T, Schillers H, Schumacher U, Prehm P. Hyaluronan export through plasma membranes depends on concurrent K+ efflux by K(ir) channels. PLOS ONE. 2012;7(6):39096. 6. https://doi.org/10.1371/journal.pone.0039096

Bibtex

@article{737803d047704f729c9929a9abb19c38,
title = "Hyaluronan export through plasma membranes depends on concurrent K+ efflux by K(ir) channels.",
abstract = "Hyaluronan is synthesized within the cytoplasm and exported into the extracellular matrix through the cell membrane of fibroblasts by the MRP5 transporter. In order to meet the law of electroneutrality, a cation is required to neutralize the emerging negative hyaluronan charges. As we previously observed an inhibiting of hyaluronan export by inhibitors of K(+) channels, hyaluronan export was now analysed by simultaneously measuring membrane potential in the presence of drugs. This was done by both hyaluronan import into inside-out vesicles and by inhibition with antisense siRNA. Hyaluronan export from fibroblast was particularly inhibited by glibenclamide, ropivacain and BaCl(2) which all belong to ATP-sensitive inwardly-rectifying K(ir) channel inhibitors. Import of hyaluronan into vesicles was activated by 150 mM KCl and this activation was abolished by ATP. siRNA for the K(+) channels K(ir)3.4 and K(ir)6.2 inhibited hyaluronan export. Collectively, these results indicated that hyaluronan export depends on concurrent K(+) efflux.",
keywords = "Humans, Reverse Transcriptase Polymerase Chain Reaction, Cell Line, Tumor, Fibroblasts, DNA Primers/genetics, Cell Membrane/*metabolism, RNA, Small Interfering/genetics, Membrane Potentials/physiology, Potassium/*metabolism, Amides/pharmacology, Barium Compounds/pharmacology, Biological Transport/drug effects/physiology, Chlorides/pharmacology, Glucuronosyltransferase/metabolism, Glyburide/pharmacology, Hyaluronic Acid/*metabolism, Potassium Channels, Inwardly Rectifying/antagonists & inhibitors/*metabolism, Potassium Chloride, RNA, Antisense/pharmacology, Transport Vesicles/metabolism, Humans, Reverse Transcriptase Polymerase Chain Reaction, Cell Line, Tumor, Fibroblasts, DNA Primers/genetics, Cell Membrane/*metabolism, RNA, Small Interfering/genetics, Membrane Potentials/physiology, Potassium/*metabolism, Amides/pharmacology, Barium Compounds/pharmacology, Biological Transport/drug effects/physiology, Chlorides/pharmacology, Glucuronosyltransferase/metabolism, Glyburide/pharmacology, Hyaluronic Acid/*metabolism, Potassium Channels, Inwardly Rectifying/antagonists & inhibitors/*metabolism, Potassium Chloride, RNA, Antisense/pharmacology, Transport Vesicles/metabolism",
author = "Daniel Hagenfeld and Beatrice Borkenhagen and Tobias Schulz and Hermann Schillers and Udo Schumacher and Peter Prehm",
year = "2012",
doi = "10.1371/journal.pone.0039096",
language = "English",
volume = "7",
pages = "39096",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - Hyaluronan export through plasma membranes depends on concurrent K+ efflux by K(ir) channels.

AU - Hagenfeld, Daniel

AU - Borkenhagen, Beatrice

AU - Schulz, Tobias

AU - Schillers, Hermann

AU - Schumacher, Udo

AU - Prehm, Peter

PY - 2012

Y1 - 2012

N2 - Hyaluronan is synthesized within the cytoplasm and exported into the extracellular matrix through the cell membrane of fibroblasts by the MRP5 transporter. In order to meet the law of electroneutrality, a cation is required to neutralize the emerging negative hyaluronan charges. As we previously observed an inhibiting of hyaluronan export by inhibitors of K(+) channels, hyaluronan export was now analysed by simultaneously measuring membrane potential in the presence of drugs. This was done by both hyaluronan import into inside-out vesicles and by inhibition with antisense siRNA. Hyaluronan export from fibroblast was particularly inhibited by glibenclamide, ropivacain and BaCl(2) which all belong to ATP-sensitive inwardly-rectifying K(ir) channel inhibitors. Import of hyaluronan into vesicles was activated by 150 mM KCl and this activation was abolished by ATP. siRNA for the K(+) channels K(ir)3.4 and K(ir)6.2 inhibited hyaluronan export. Collectively, these results indicated that hyaluronan export depends on concurrent K(+) efflux.

AB - Hyaluronan is synthesized within the cytoplasm and exported into the extracellular matrix through the cell membrane of fibroblasts by the MRP5 transporter. In order to meet the law of electroneutrality, a cation is required to neutralize the emerging negative hyaluronan charges. As we previously observed an inhibiting of hyaluronan export by inhibitors of K(+) channels, hyaluronan export was now analysed by simultaneously measuring membrane potential in the presence of drugs. This was done by both hyaluronan import into inside-out vesicles and by inhibition with antisense siRNA. Hyaluronan export from fibroblast was particularly inhibited by glibenclamide, ropivacain and BaCl(2) which all belong to ATP-sensitive inwardly-rectifying K(ir) channel inhibitors. Import of hyaluronan into vesicles was activated by 150 mM KCl and this activation was abolished by ATP. siRNA for the K(+) channels K(ir)3.4 and K(ir)6.2 inhibited hyaluronan export. Collectively, these results indicated that hyaluronan export depends on concurrent K(+) efflux.

KW - Humans

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Cell Line, Tumor

KW - Fibroblasts

KW - DNA Primers/genetics

KW - Cell Membrane/metabolism

KW - RNA, Small Interfering/genetics

KW - Membrane Potentials/physiology

KW - Potassium/metabolism

KW - Amides/pharmacology

KW - Barium Compounds/pharmacology

KW - Biological Transport/drug effects/physiology

KW - Chlorides/pharmacology

KW - Glucuronosyltransferase/metabolism

KW - Glyburide/pharmacology

KW - Hyaluronic Acid/metabolism

KW - Potassium Channels, Inwardly Rectifying/antagonists & inhibitors/metabolism

KW - Potassium Chloride

KW - RNA, Antisense/pharmacology

KW - Transport Vesicles/metabolism

KW - Humans

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Cell Line, Tumor

KW - Fibroblasts

KW - DNA Primers/genetics

KW - Cell Membrane/metabolism

KW - RNA, Small Interfering/genetics

KW - Membrane Potentials/physiology

KW - Potassium/metabolism

KW - Amides/pharmacology

KW - Barium Compounds/pharmacology

KW - Biological Transport/drug effects/physiology

KW - Chlorides/pharmacology

KW - Glucuronosyltransferase/metabolism

KW - Glyburide/pharmacology

KW - Hyaluronic Acid/metabolism

KW - Potassium Channels, Inwardly Rectifying/antagonists & inhibitors/metabolism

KW - Potassium Chloride

KW - RNA, Antisense/pharmacology

KW - Transport Vesicles/metabolism

U2 - 10.1371/journal.pone.0039096

DO - 10.1371/journal.pone.0039096

M3 - SCORING: Journal article

VL - 7

SP - 39096

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 6

M1 - 6

ER -