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Human native Cav1 channels in chromaffin cells: contribution to exocytosis and firing of spontaneous action potentials

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Human native Cav1 channels in chromaffin cells: contribution to exocytosis and firing of spontaneous action potentials. / Hernández-Vivanco, Alicia; Sanz-Lázaro, Sara; Jiménez-Pompa, Amanda; García-Magro, Nuria; Carmona-Hidalgo, Beatriz; Pérez-Alvarez, Alberto; Caba-González, Jose Carlos; Tabernero, Angel; Alonso Y Gregorio, Sergio; Passas, Juan; Blázquez, Jesús; González-Enguita, Carmen; de Castro-Guerín, Cristina; Albillos, Almudena.

In: EUR J PHARMACOL, Vol. 796, 02.2017, p. 115-121.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hernández-Vivanco, A, Sanz-Lázaro, S, Jiménez-Pompa, A, García-Magro, N, Carmona-Hidalgo, B, Pérez-Alvarez, A, Caba-González, JC, Tabernero, A, Alonso Y Gregorio, S, Passas, J, Blázquez, J, González-Enguita, C, de Castro-Guerín, C & Albillos, A 2017, 'Human native Cav1 channels in chromaffin cells: contribution to exocytosis and firing of spontaneous action potentials', EUR J PHARMACOL, vol. 796, pp. 115-121. https://doi.org/10.1016/j.ejphar.2016.12.009

APA

Hernández-Vivanco, A., Sanz-Lázaro, S., Jiménez-Pompa, A., García-Magro, N., Carmona-Hidalgo, B., Pérez-Alvarez, A., Caba-González, J. C., Tabernero, A., Alonso Y Gregorio, S., Passas, J., Blázquez, J., González-Enguita, C., de Castro-Guerín, C., & Albillos, A. (2017). Human native Cav1 channels in chromaffin cells: contribution to exocytosis and firing of spontaneous action potentials. EUR J PHARMACOL, 796, 115-121. https://doi.org/10.1016/j.ejphar.2016.12.009

Vancouver

Hernández-Vivanco A, Sanz-Lázaro S, Jiménez-Pompa A, García-Magro N, Carmona-Hidalgo B, Pérez-Alvarez A et al. Human native Cav1 channels in chromaffin cells: contribution to exocytosis and firing of spontaneous action potentials. EUR J PHARMACOL. 2017 Feb;796:115-121. https://doi.org/10.1016/j.ejphar.2016.12.009

Bibtex

@article{2f549fa07d8749efa0593b3b5b4c798f,
title = "Human native Cav1 channels in chromaffin cells: contribution to exocytosis and firing of spontaneous action potentials",
abstract = "The present study was performed to evaluate the Cav1 channel subtypes expressed in human chromaffin cells and the role that these channels play in exocytosis and cell excitability. Here we show that human chromaffin cells obtained from organ donors express Cav1.2 and Cav1.3 subtypes using molecular and pharmacological techniques. Immunocytochemical data demonstrated the presence of Cav1.2 and Cav1.3 subtypes, but not Cav1.1 or Cav1.4. Electrophysiological experiments were conducted to investigate the contribution of Cav1 channels to the exocytotic process and cell excitability. Cav1 channels contribute to the exocytosis of secretory vesicles, evidenced by the block of 3μM nifedipine (36.5±2%) of membrane capacitance increment elicited by 200ms depolarizing pulses. These channels show a minor contribution to the initiation of spontaneous action potential firing, as shown by the 2.5 pA of current at the threshold potential (-34mV), which elicits 10.4mV of potential increment. In addition, we found that only 8% of human chromaffin cells exhibit spontaneous action potentials. These data offer novel information regarding human chromaffin cells and the role of human native Cav1 channels in exocytosis and cell excitability.",
author = "Alicia Hern{\'a}ndez-Vivanco and Sara Sanz-L{\'a}zaro and Amanda Jim{\'e}nez-Pompa and Nuria Garc{\'i}a-Magro and Beatriz Carmona-Hidalgo and Alberto P{\'e}rez-Alvarez and Caba-Gonz{\'a}lez, {Jose Carlos} and Angel Tabernero and {Alonso Y Gregorio}, Sergio and Juan Passas and Jes{\'u}s Bl{\'a}zquez and Carmen Gonz{\'a}lez-Enguita and {de Castro-Guer{\'i}n}, Cristina and Almudena Albillos",
note = "Copyright {\textcopyright} 2016 Elsevier B.V. All rights reserved.",
year = "2017",
month = feb,
doi = "10.1016/j.ejphar.2016.12.009",
language = "English",
volume = "796",
pages = "115--121",
journal = "EUR J PHARMACOL",
issn = "0014-2999",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Human native Cav1 channels in chromaffin cells: contribution to exocytosis and firing of spontaneous action potentials

AU - Hernández-Vivanco, Alicia

AU - Sanz-Lázaro, Sara

AU - Jiménez-Pompa, Amanda

AU - García-Magro, Nuria

AU - Carmona-Hidalgo, Beatriz

AU - Pérez-Alvarez, Alberto

AU - Caba-González, Jose Carlos

AU - Tabernero, Angel

AU - Alonso Y Gregorio, Sergio

AU - Passas, Juan

AU - Blázquez, Jesús

AU - González-Enguita, Carmen

AU - de Castro-Guerín, Cristina

AU - Albillos, Almudena

N1 - Copyright © 2016 Elsevier B.V. All rights reserved.

PY - 2017/2

Y1 - 2017/2

N2 - The present study was performed to evaluate the Cav1 channel subtypes expressed in human chromaffin cells and the role that these channels play in exocytosis and cell excitability. Here we show that human chromaffin cells obtained from organ donors express Cav1.2 and Cav1.3 subtypes using molecular and pharmacological techniques. Immunocytochemical data demonstrated the presence of Cav1.2 and Cav1.3 subtypes, but not Cav1.1 or Cav1.4. Electrophysiological experiments were conducted to investigate the contribution of Cav1 channels to the exocytotic process and cell excitability. Cav1 channels contribute to the exocytosis of secretory vesicles, evidenced by the block of 3μM nifedipine (36.5±2%) of membrane capacitance increment elicited by 200ms depolarizing pulses. These channels show a minor contribution to the initiation of spontaneous action potential firing, as shown by the 2.5 pA of current at the threshold potential (-34mV), which elicits 10.4mV of potential increment. In addition, we found that only 8% of human chromaffin cells exhibit spontaneous action potentials. These data offer novel information regarding human chromaffin cells and the role of human native Cav1 channels in exocytosis and cell excitability.

AB - The present study was performed to evaluate the Cav1 channel subtypes expressed in human chromaffin cells and the role that these channels play in exocytosis and cell excitability. Here we show that human chromaffin cells obtained from organ donors express Cav1.2 and Cav1.3 subtypes using molecular and pharmacological techniques. Immunocytochemical data demonstrated the presence of Cav1.2 and Cav1.3 subtypes, but not Cav1.1 or Cav1.4. Electrophysiological experiments were conducted to investigate the contribution of Cav1 channels to the exocytotic process and cell excitability. Cav1 channels contribute to the exocytosis of secretory vesicles, evidenced by the block of 3μM nifedipine (36.5±2%) of membrane capacitance increment elicited by 200ms depolarizing pulses. These channels show a minor contribution to the initiation of spontaneous action potential firing, as shown by the 2.5 pA of current at the threshold potential (-34mV), which elicits 10.4mV of potential increment. In addition, we found that only 8% of human chromaffin cells exhibit spontaneous action potentials. These data offer novel information regarding human chromaffin cells and the role of human native Cav1 channels in exocytosis and cell excitability.

U2 - 10.1016/j.ejphar.2016.12.009

DO - 10.1016/j.ejphar.2016.12.009

M3 - SCORING: Journal article

C2 - 27988286

VL - 796

SP - 115

EP - 121

JO - EUR J PHARMACOL

JF - EUR J PHARMACOL

SN - 0014-2999

ER -