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Human intestine luminal ACE2 and amino acid transporter expression increased by ACE-inhibitors

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Human intestine luminal ACE2 and amino acid transporter expression increased by ACE-inhibitors. / Vuille-dit-Bille, Raphael N; Camargo, Simone M; Emmenegger, Luca; Sasse, Tom; Kummer, Eva; Jando, Julia; Hamie, Qeumars M; Meier, Chantal F; Hunziker, Schirin; Forras-Kaufmann, Zsofia; Kuyumcu, Sena; Fox, Mark; Schwizer, Werner; Fried, Michael; Lindenmeyer, Maja; Götze, Oliver; Verrey, François.

In: AMINO ACIDS, Vol. 47, No. 4, 04.2015, p. 693-705.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Vuille-dit-Bille, RN, Camargo, SM, Emmenegger, L, Sasse, T, Kummer, E, Jando, J, Hamie, QM, Meier, CF, Hunziker, S, Forras-Kaufmann, Z, Kuyumcu, S, Fox, M, Schwizer, W, Fried, M, Lindenmeyer, M, Götze, O & Verrey, F 2015, 'Human intestine luminal ACE2 and amino acid transporter expression increased by ACE-inhibitors', AMINO ACIDS, vol. 47, no. 4, pp. 693-705. https://doi.org/10.1007/s00726-014-1889-6

APA

Vuille-dit-Bille, R. N., Camargo, S. M., Emmenegger, L., Sasse, T., Kummer, E., Jando, J., Hamie, Q. M., Meier, C. F., Hunziker, S., Forras-Kaufmann, Z., Kuyumcu, S., Fox, M., Schwizer, W., Fried, M., Lindenmeyer, M., Götze, O., & Verrey, F. (2015). Human intestine luminal ACE2 and amino acid transporter expression increased by ACE-inhibitors. AMINO ACIDS, 47(4), 693-705. https://doi.org/10.1007/s00726-014-1889-6

Vancouver

Vuille-dit-Bille RN, Camargo SM, Emmenegger L, Sasse T, Kummer E, Jando J et al. Human intestine luminal ACE2 and amino acid transporter expression increased by ACE-inhibitors. AMINO ACIDS. 2015 Apr;47(4):693-705. https://doi.org/10.1007/s00726-014-1889-6

Bibtex

@article{57f4a200092d4f25926def15bf88ac24,
title = "Human intestine luminal ACE2 and amino acid transporter expression increased by ACE-inhibitors",
abstract = "Sodium-dependent neutral amino acid transporter B(0)AT1 (SLC6A19) and imino acid (proline) transporter SIT1 (SLC6A20) are expressed at the luminal membrane of small intestine enterocytes and proximal tubule kidney cells where they exert key functions for amino acid (re)absorption as documented by their role in Hartnup disorder and iminoglycinuria, respectively. Expression of B(0)AT1 was shown in rodent intestine to depend on the presence of the carboxypeptidase angiotensin-converting enzyme 2 (ACE2). This enzyme belongs to the renin-angiotensin system and its expression is induced by treatment with ACE-inhibitors (ACEIs) or angiotensin II AT1 receptor blockers (ARBs) in many rodent tissues. We show here in the Xenopus laevis oocyte expression system that human ACE2 also functionally interacts with SIT1. To investigate in human intestine the potential effect of ACEIs or ARBs on ACE2, we analysed intestinal biopsies taken during routine gastroduodenoscopy and ileocolonoscopy from 46 patients of which 9 were under ACEI and 13 ARB treatment. Analysis of transcript expression by real-time PCR and of proteins by immunofluorescence showed a co-localization of SIT1 and B(0)AT1 with ACE2 in the brush-border membrane of human small intestine enterocytes and a distinct axial expression pattern of the tested gene products along the intestine. Patients treated with ACEIs displayed in comparison with untreated controls increased intestinal mRNA levels of ACE2, peptide transporter PEPT1 (SLC15A1) and AA transporters B(0)AT1 and PAT1 (SLC36A1). This study unravels in human intestine the localization and distribution of intestinal transporters involved in amino acid absorption and suggests that ACEIs impact on their expression. ",
keywords = "Amino Acid Transport Systems, Neutral, Angiotensin-Converting Enzyme Inhibitors, Animals, Gene Expression, Humans, Intestinal Mucosa, Intestines, Membrane Transport Proteins, Peptidyl-Dipeptidase A, Protein Transport, Up-Regulation, Xenopus laevis, Journal Article, Research Support, Non-U.S. Gov't",
author = "Vuille-dit-Bille, {Raphael N} and Camargo, {Simone M} and Luca Emmenegger and Tom Sasse and Eva Kummer and Julia Jando and Hamie, {Qeumars M} and Meier, {Chantal F} and Schirin Hunziker and Zsofia Forras-Kaufmann and Sena Kuyumcu and Mark Fox and Werner Schwizer and Michael Fried and Maja Lindenmeyer and Oliver G{\"o}tze and Fran{\c c}ois Verrey",
year = "2015",
month = apr,
doi = "10.1007/s00726-014-1889-6",
language = "English",
volume = "47",
pages = "693--705",
journal = "AMINO ACIDS",
issn = "0939-4451",
publisher = "Springer Wien",
number = "4",

}

RIS

TY - JOUR

T1 - Human intestine luminal ACE2 and amino acid transporter expression increased by ACE-inhibitors

AU - Vuille-dit-Bille, Raphael N

AU - Camargo, Simone M

AU - Emmenegger, Luca

AU - Sasse, Tom

AU - Kummer, Eva

AU - Jando, Julia

AU - Hamie, Qeumars M

AU - Meier, Chantal F

AU - Hunziker, Schirin

AU - Forras-Kaufmann, Zsofia

AU - Kuyumcu, Sena

AU - Fox, Mark

AU - Schwizer, Werner

AU - Fried, Michael

AU - Lindenmeyer, Maja

AU - Götze, Oliver

AU - Verrey, François

PY - 2015/4

Y1 - 2015/4

N2 - Sodium-dependent neutral amino acid transporter B(0)AT1 (SLC6A19) and imino acid (proline) transporter SIT1 (SLC6A20) are expressed at the luminal membrane of small intestine enterocytes and proximal tubule kidney cells where they exert key functions for amino acid (re)absorption as documented by their role in Hartnup disorder and iminoglycinuria, respectively. Expression of B(0)AT1 was shown in rodent intestine to depend on the presence of the carboxypeptidase angiotensin-converting enzyme 2 (ACE2). This enzyme belongs to the renin-angiotensin system and its expression is induced by treatment with ACE-inhibitors (ACEIs) or angiotensin II AT1 receptor blockers (ARBs) in many rodent tissues. We show here in the Xenopus laevis oocyte expression system that human ACE2 also functionally interacts with SIT1. To investigate in human intestine the potential effect of ACEIs or ARBs on ACE2, we analysed intestinal biopsies taken during routine gastroduodenoscopy and ileocolonoscopy from 46 patients of which 9 were under ACEI and 13 ARB treatment. Analysis of transcript expression by real-time PCR and of proteins by immunofluorescence showed a co-localization of SIT1 and B(0)AT1 with ACE2 in the brush-border membrane of human small intestine enterocytes and a distinct axial expression pattern of the tested gene products along the intestine. Patients treated with ACEIs displayed in comparison with untreated controls increased intestinal mRNA levels of ACE2, peptide transporter PEPT1 (SLC15A1) and AA transporters B(0)AT1 and PAT1 (SLC36A1). This study unravels in human intestine the localization and distribution of intestinal transporters involved in amino acid absorption and suggests that ACEIs impact on their expression.

AB - Sodium-dependent neutral amino acid transporter B(0)AT1 (SLC6A19) and imino acid (proline) transporter SIT1 (SLC6A20) are expressed at the luminal membrane of small intestine enterocytes and proximal tubule kidney cells where they exert key functions for amino acid (re)absorption as documented by their role in Hartnup disorder and iminoglycinuria, respectively. Expression of B(0)AT1 was shown in rodent intestine to depend on the presence of the carboxypeptidase angiotensin-converting enzyme 2 (ACE2). This enzyme belongs to the renin-angiotensin system and its expression is induced by treatment with ACE-inhibitors (ACEIs) or angiotensin II AT1 receptor blockers (ARBs) in many rodent tissues. We show here in the Xenopus laevis oocyte expression system that human ACE2 also functionally interacts with SIT1. To investigate in human intestine the potential effect of ACEIs or ARBs on ACE2, we analysed intestinal biopsies taken during routine gastroduodenoscopy and ileocolonoscopy from 46 patients of which 9 were under ACEI and 13 ARB treatment. Analysis of transcript expression by real-time PCR and of proteins by immunofluorescence showed a co-localization of SIT1 and B(0)AT1 with ACE2 in the brush-border membrane of human small intestine enterocytes and a distinct axial expression pattern of the tested gene products along the intestine. Patients treated with ACEIs displayed in comparison with untreated controls increased intestinal mRNA levels of ACE2, peptide transporter PEPT1 (SLC15A1) and AA transporters B(0)AT1 and PAT1 (SLC36A1). This study unravels in human intestine the localization and distribution of intestinal transporters involved in amino acid absorption and suggests that ACEIs impact on their expression.

KW - Amino Acid Transport Systems, Neutral

KW - Angiotensin-Converting Enzyme Inhibitors

KW - Animals

KW - Gene Expression

KW - Humans

KW - Intestinal Mucosa

KW - Intestines

KW - Membrane Transport Proteins

KW - Peptidyl-Dipeptidase A

KW - Protein Transport

KW - Up-Regulation

KW - Xenopus laevis

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s00726-014-1889-6

DO - 10.1007/s00726-014-1889-6

M3 - SCORING: Journal article

C2 - 25534429

VL - 47

SP - 693

EP - 705

JO - AMINO ACIDS

JF - AMINO ACIDS

SN - 0939-4451

IS - 4

ER -