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Human engineered heart tissue transplantation in a guinea pig chronic injury model

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Human engineered heart tissue transplantation in a guinea pig chronic injury model. / von Bibra, Constantin; Shibamiya, Aya; Geertz, Birgit; Querdel, Eva; Köhne, Maria; Stüdemann, Tim; Starbatty, Jutta; Schmidt, Felix N; Hansen, Arne; Hiebl, Bernhard; Eschenhagen, Thomas; Weinberger, Florian.

In: J MOL CELL CARDIOL, Vol. 166, 23.01.2022, p. 1-10.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

von Bibra, C, Shibamiya, A, Geertz, B, Querdel, E, Köhne, M, Stüdemann, T, Starbatty, J, Schmidt, FN, Hansen, A, Hiebl, B, Eschenhagen, T & Weinberger, F 2022, 'Human engineered heart tissue transplantation in a guinea pig chronic injury model', J MOL CELL CARDIOL, vol. 166, pp. 1-10. https://doi.org/10.1016/j.yjmcc.2022.01.007

APA

von Bibra, C., Shibamiya, A., Geertz, B., Querdel, E., Köhne, M., Stüdemann, T., Starbatty, J., Schmidt, F. N., Hansen, A., Hiebl, B., Eschenhagen, T., & Weinberger, F. (2022). Human engineered heart tissue transplantation in a guinea pig chronic injury model. J MOL CELL CARDIOL, 166, 1-10. https://doi.org/10.1016/j.yjmcc.2022.01.007

Vancouver

von Bibra C, Shibamiya A, Geertz B, Querdel E, Köhne M, Stüdemann T et al. Human engineered heart tissue transplantation in a guinea pig chronic injury model. J MOL CELL CARDIOL. 2022 Jan 23;166:1-10. https://doi.org/10.1016/j.yjmcc.2022.01.007

Bibtex

@article{756ccd04ae214c2bb25570ecd1de01c6,
title = "Human engineered heart tissue transplantation in a guinea pig chronic injury model",
abstract = "Myocardial injury leads to an irreversible loss of cardiomyocytes (CM). The implantation of human engineered heart tissue (EHT) has become a promising regenerative approach. Previous studies exhibited beneficial, dose-dependent effects of human induced pluripotent stem cell (hiPSC)-derived EHT patch transplantation in a guinea pig model in the subacute phase of myocardial injury. Yet, advanced heart failure often results from a chronic remodeling process. Therefore, from a clinical standpoint it is worthwhile to explore the ability to repair the chronically injured heart. In this study human EHT patches were generated from hiPSC-derived CMs (15 × 106 cells) and implanted epicardially four weeks after injury in a guinea pig cryo-injury model. Cardiac function was evaluated by echocardiography after a follow-up period of four weeks. Hearts revealed large transmural myocardial injuries amounting to 27% of the left ventricle. EHT recipient hearts demonstrated compact muscle islands of human origin in the scar region, as indicated by a positive staining for human Ku80 and dystrophin, remuscularizing 5% of the scar area. Echocardiographic analysis demonstrated no significant functional difference between animals that received EHT patches and animals in the cell-free control group (fractional area change 36% vs. 34%). Thus, EHT patches engrafted in the chronically injured heart but in contrast to the subacute model, grafts were smaller and EHT patch transplantation did not improve left ventricular function, highlighting the difficulties for a regenerative approach.",
keywords = "Animals, Cicatrix, Guinea Pigs, Heart Ventricles, Humans, Induced Pluripotent Stem Cells, Myocytes, Cardiac/transplantation, Tissue Engineering/methods",
author = "{von Bibra}, Constantin and Aya Shibamiya and Birgit Geertz and Eva Querdel and Maria K{\"o}hne and Tim St{\"u}demann and Jutta Starbatty and Schmidt, {Felix N} and Arne Hansen and Bernhard Hiebl and Thomas Eschenhagen and Florian Weinberger",
note = "Copyright {\textcopyright} 2022. Published by Elsevier Ltd.",
year = "2022",
month = jan,
day = "23",
doi = "10.1016/j.yjmcc.2022.01.007",
language = "English",
volume = "166",
pages = "1--10",
journal = "J MOL CELL CARDIOL",
issn = "0022-2828",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Human engineered heart tissue transplantation in a guinea pig chronic injury model

AU - von Bibra, Constantin

AU - Shibamiya, Aya

AU - Geertz, Birgit

AU - Querdel, Eva

AU - Köhne, Maria

AU - Stüdemann, Tim

AU - Starbatty, Jutta

AU - Schmidt, Felix N

AU - Hansen, Arne

AU - Hiebl, Bernhard

AU - Eschenhagen, Thomas

AU - Weinberger, Florian

N1 - Copyright © 2022. Published by Elsevier Ltd.

PY - 2022/1/23

Y1 - 2022/1/23

N2 - Myocardial injury leads to an irreversible loss of cardiomyocytes (CM). The implantation of human engineered heart tissue (EHT) has become a promising regenerative approach. Previous studies exhibited beneficial, dose-dependent effects of human induced pluripotent stem cell (hiPSC)-derived EHT patch transplantation in a guinea pig model in the subacute phase of myocardial injury. Yet, advanced heart failure often results from a chronic remodeling process. Therefore, from a clinical standpoint it is worthwhile to explore the ability to repair the chronically injured heart. In this study human EHT patches were generated from hiPSC-derived CMs (15 × 106 cells) and implanted epicardially four weeks after injury in a guinea pig cryo-injury model. Cardiac function was evaluated by echocardiography after a follow-up period of four weeks. Hearts revealed large transmural myocardial injuries amounting to 27% of the left ventricle. EHT recipient hearts demonstrated compact muscle islands of human origin in the scar region, as indicated by a positive staining for human Ku80 and dystrophin, remuscularizing 5% of the scar area. Echocardiographic analysis demonstrated no significant functional difference between animals that received EHT patches and animals in the cell-free control group (fractional area change 36% vs. 34%). Thus, EHT patches engrafted in the chronically injured heart but in contrast to the subacute model, grafts were smaller and EHT patch transplantation did not improve left ventricular function, highlighting the difficulties for a regenerative approach.

AB - Myocardial injury leads to an irreversible loss of cardiomyocytes (CM). The implantation of human engineered heart tissue (EHT) has become a promising regenerative approach. Previous studies exhibited beneficial, dose-dependent effects of human induced pluripotent stem cell (hiPSC)-derived EHT patch transplantation in a guinea pig model in the subacute phase of myocardial injury. Yet, advanced heart failure often results from a chronic remodeling process. Therefore, from a clinical standpoint it is worthwhile to explore the ability to repair the chronically injured heart. In this study human EHT patches were generated from hiPSC-derived CMs (15 × 106 cells) and implanted epicardially four weeks after injury in a guinea pig cryo-injury model. Cardiac function was evaluated by echocardiography after a follow-up period of four weeks. Hearts revealed large transmural myocardial injuries amounting to 27% of the left ventricle. EHT recipient hearts demonstrated compact muscle islands of human origin in the scar region, as indicated by a positive staining for human Ku80 and dystrophin, remuscularizing 5% of the scar area. Echocardiographic analysis demonstrated no significant functional difference between animals that received EHT patches and animals in the cell-free control group (fractional area change 36% vs. 34%). Thus, EHT patches engrafted in the chronically injured heart but in contrast to the subacute model, grafts were smaller and EHT patch transplantation did not improve left ventricular function, highlighting the difficulties for a regenerative approach.

KW - Animals

KW - Cicatrix

KW - Guinea Pigs

KW - Heart Ventricles

KW - Humans

KW - Induced Pluripotent Stem Cells

KW - Myocytes, Cardiac/transplantation

KW - Tissue Engineering/methods

U2 - 10.1016/j.yjmcc.2022.01.007

DO - 10.1016/j.yjmcc.2022.01.007

M3 - SCORING: Journal article

C2 - 35081367

VL - 166

SP - 1

EP - 10

JO - J MOL CELL CARDIOL

JF - J MOL CELL CARDIOL

SN - 0022-2828

ER -