Research ExplorerPublicationsHuman Engineered Heart Tissue: Analysis of Contractile Force

Human Engineered Heart Tissue: Analysis of Contractile Force

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Human Engineered Heart Tissue: Analysis of Contractile Force. / Mannhardt, Ingra; Breckwoldt, Kaja; Letuffe-Brenière, David; Schaaf, Sebastian; Schulz, Herbert; Neuber, Christiane; Benzin, Anika; Werner, Tessa; Eder, Alexandra; Schulze, Thomas; Klampe, Birgit; Christ, Torsten; Hirt, Marc N; Huebner, Norbert; Moretti, Alessandra; Eschenhagen, Thomas; Hansen, Arne.

In: STEM CELL REP, Vol. 7, No. 1, 12.07.2016, p. 29-42.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Mannhardt, I, Breckwoldt, K, Letuffe-Brenière, D, Schaaf, S, Schulz, H, Neuber, C, Benzin, A, Werner, T, Eder, A, Schulze, T, Klampe, B, Christ, T, Hirt, MN, Huebner, N, Moretti, A, Eschenhagen, T & Hansen, A 2016, 'Human Engineered Heart Tissue: Analysis of Contractile Force', STEM CELL REP, vol. 7, no. 1, pp. 29-42. https://doi.org/10.1016/j.stemcr.2016.04.011

APA

Mannhardt, I., Breckwoldt, K., Letuffe-Brenière, D., Schaaf, S., Schulz, H., Neuber, C., Benzin, A., Werner, T., Eder, A., Schulze, T., Klampe, B., Christ, T., Hirt, M. N., Huebner, N., Moretti, A., Eschenhagen, T., & Hansen, A. (2016). Human Engineered Heart Tissue: Analysis of Contractile Force. STEM CELL REP, 7(1), 29-42. https://doi.org/10.1016/j.stemcr.2016.04.011

Vancouver

Mannhardt I, Breckwoldt K, Letuffe-Brenière D, Schaaf S, Schulz H, Neuber C et al. Human Engineered Heart Tissue: Analysis of Contractile Force. STEM CELL REP. 2016 Jul 12;7(1):29-42. https://doi.org/10.1016/j.stemcr.2016.04.011

Bibtex

@article{f82823b996b64426b30fbc5410eabbbc,
title = "Human Engineered Heart Tissue: Analysis of Contractile Force",
abstract = "Analyzing contractile force, the most important and best understood function of cardiomyocytes in vivo is not established in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM). This study describes the generation of 3D, strip-format, force-generating engineered heart tissues (EHT) from hiPSC-CM and their physiological and pharmacological properties. CM were differentiated from hiPSC by a growth factor-based three-stage protocol. EHTs were generated and analyzed histologically and functionally. HiPSC-CM in EHTs showed well-developed sarcomeric organization and alignment, and frequent mitochondria. Systematic contractility analysis (26 concentration-response curves) reveals that EHTs replicated canonical response to physiological and pharmacological regulators of inotropy, membrane- and calcium-clock mediators of pacemaking, modulators of ion-channel currents, and proarrhythmic compounds with unprecedented precision. The analysis demonstrates a high degree of similarity between hiPSC-CM in EHT format and native human heart tissue, indicating that human EHTs are useful for preclinical drug testing and disease modeling.",
keywords = "Journal Article",
author = "Ingra Mannhardt and Kaja Breckwoldt and David Letuffe-Breni{\`e}re and Sebastian Schaaf and Herbert Schulz and Christiane Neuber and Anika Benzin and Tessa Werner and Alexandra Eder and Thomas Schulze and Birgit Klampe and Torsten Christ and Hirt, {Marc N} and Norbert Huebner and Alessandra Moretti and Thomas Eschenhagen and Arne Hansen",
note = "Copyright {\textcopyright} 2016 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2016",
month = jul,
day = "12",
doi = "10.1016/j.stemcr.2016.04.011",
language = "English",
volume = "7",
pages = "29--42",
journal = "STEM CELL REP",
issn = "2213-6711",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - Human Engineered Heart Tissue: Analysis of Contractile Force

AU - Mannhardt, Ingra

AU - Breckwoldt, Kaja

AU - Letuffe-Brenière, David

AU - Schaaf, Sebastian

AU - Schulz, Herbert

AU - Neuber, Christiane

AU - Benzin, Anika

AU - Werner, Tessa

AU - Eder, Alexandra

AU - Schulze, Thomas

AU - Klampe, Birgit

AU - Christ, Torsten

AU - Hirt, Marc N

AU - Huebner, Norbert

AU - Moretti, Alessandra

AU - Eschenhagen, Thomas

AU - Hansen, Arne

N1 - Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2016/7/12

Y1 - 2016/7/12

N2 - Analyzing contractile force, the most important and best understood function of cardiomyocytes in vivo is not established in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM). This study describes the generation of 3D, strip-format, force-generating engineered heart tissues (EHT) from hiPSC-CM and their physiological and pharmacological properties. CM were differentiated from hiPSC by a growth factor-based three-stage protocol. EHTs were generated and analyzed histologically and functionally. HiPSC-CM in EHTs showed well-developed sarcomeric organization and alignment, and frequent mitochondria. Systematic contractility analysis (26 concentration-response curves) reveals that EHTs replicated canonical response to physiological and pharmacological regulators of inotropy, membrane- and calcium-clock mediators of pacemaking, modulators of ion-channel currents, and proarrhythmic compounds with unprecedented precision. The analysis demonstrates a high degree of similarity between hiPSC-CM in EHT format and native human heart tissue, indicating that human EHTs are useful for preclinical drug testing and disease modeling.

AB - Analyzing contractile force, the most important and best understood function of cardiomyocytes in vivo is not established in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM). This study describes the generation of 3D, strip-format, force-generating engineered heart tissues (EHT) from hiPSC-CM and their physiological and pharmacological properties. CM were differentiated from hiPSC by a growth factor-based three-stage protocol. EHTs were generated and analyzed histologically and functionally. HiPSC-CM in EHTs showed well-developed sarcomeric organization and alignment, and frequent mitochondria. Systematic contractility analysis (26 concentration-response curves) reveals that EHTs replicated canonical response to physiological and pharmacological regulators of inotropy, membrane- and calcium-clock mediators of pacemaking, modulators of ion-channel currents, and proarrhythmic compounds with unprecedented precision. The analysis demonstrates a high degree of similarity between hiPSC-CM in EHT format and native human heart tissue, indicating that human EHTs are useful for preclinical drug testing and disease modeling.

KW - Journal Article

U2 - 10.1016/j.stemcr.2016.04.011

DO - 10.1016/j.stemcr.2016.04.011

M3 - SCORING: Journal article

C2 - 27211213

VL - 7

SP - 29

EP - 42

JO - STEM CELL REP

JF - STEM CELL REP

SN - 2213-6711

IS - 1

ER -