Human endogenous retrovirus (HERV)-K transcripts in germ cell and trophoblastic tumours.
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Human endogenous retrovirus (HERV)-K transcripts in germ cell and trophoblastic tumours. / Herbst, H; Sauter, M; Kühler-Obbarius, C; Löning, Thomas; Mueller-Lantzsch, N.
In: APMIS, Vol. 106, No. 1, 1, 1998, p. 216-220.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Human endogenous retrovirus (HERV)-K transcripts in germ cell and trophoblastic tumours.
AU - Herbst, H
AU - Sauter, M
AU - Kühler-Obbarius, C
AU - Löning, Thomas
AU - Mueller-Lantzsch, N
PY - 1998
Y1 - 1998
N2 - Prompted by the observation of retroviral particle formation in teratocarcinoma cell lines and the consistent finding of antibodies against Gag and Env proteins encoded by human endogenous retrovirus (HERV)-K genomes in the sera of patients with classical seminoma, we studied ovarian and testicular germ cell tumours, their precursor lesions, dysgenetic gonads, and trophoblast lesions for expression of HERV-K sequences by in situ hybridization using radioactive and non-radioactive probes. HERV-K transcripts were detected in all testicular and ovarian germ cell tumours with the exception of teratomas and spermatocytic seminomas. HERV-K expression was also common to testicular carcinoma in situ as well as gonocytes of dysgenetic gonads. Among gestational trophoblastic lesions, HERV-K expression was regularly found in choriocarcinomas, but not in molar lesions. The patterns of HERV-K expression suggest a common molecular pathogenesis of most germ cell tumour entities and malignant gestational trophoblastic disease. They furthermore support the concept of carcinoma in situ as a precursor lesion common to most testicular germ cell neoplasms. The detection of HERV-K gene products in body fluids and tissues may aid diagnosis and monitoring of germ cell tumours and related lesions.
AB - Prompted by the observation of retroviral particle formation in teratocarcinoma cell lines and the consistent finding of antibodies against Gag and Env proteins encoded by human endogenous retrovirus (HERV)-K genomes in the sera of patients with classical seminoma, we studied ovarian and testicular germ cell tumours, their precursor lesions, dysgenetic gonads, and trophoblast lesions for expression of HERV-K sequences by in situ hybridization using radioactive and non-radioactive probes. HERV-K transcripts were detected in all testicular and ovarian germ cell tumours with the exception of teratomas and spermatocytic seminomas. HERV-K expression was also common to testicular carcinoma in situ as well as gonocytes of dysgenetic gonads. Among gestational trophoblastic lesions, HERV-K expression was regularly found in choriocarcinomas, but not in molar lesions. The patterns of HERV-K expression suggest a common molecular pathogenesis of most germ cell tumour entities and malignant gestational trophoblastic disease. They furthermore support the concept of carcinoma in situ as a precursor lesion common to most testicular germ cell neoplasms. The detection of HERV-K gene products in body fluids and tissues may aid diagnosis and monitoring of germ cell tumours and related lesions.
M3 - SCORING: Zeitschriftenaufsatz
VL - 106
SP - 216
EP - 220
JO - APMIS
JF - APMIS
SN - 0903-4641
IS - 1
M1 - 1
ER -
