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Human cytomegalovirus induces upregulation of arginase II possible implications for vasculopathies

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Human cytomegalovirus induces upregulation of arginase II possible implications for vasculopathies. / Yaiw, Koon-Chu; Mohammad, Abdul-Aleem; Taher, Chato; Wilhelmi, Vanessa; Davoudi, Belghis; Strååt, Klas; Assinger, Alice; Ovchinnikova, Olga; Shlyakhto, Eugene; Rahbar, Afsar; Koutonguk, Oksana; Religa, Piotr; Butler, Lynn; Khan, Zahidul; Streblow, Daniel; Pernow, John; Söderberg-Nauclér, Cecilia.

In: BASIC RES CARDIOL, Vol. 109, No. 2, 01.03.2014, p. 401.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Yaiw, K-C, Mohammad, A-A, Taher, C, Wilhelmi, V, Davoudi, B, Strååt, K, Assinger, A, Ovchinnikova, O, Shlyakhto, E, Rahbar, A, Koutonguk, O, Religa, P, Butler, L, Khan, Z, Streblow, D, Pernow, J & Söderberg-Nauclér, C 2014, 'Human cytomegalovirus induces upregulation of arginase II possible implications for vasculopathies', BASIC RES CARDIOL, vol. 109, no. 2, pp. 401. https://doi.org/10.1007/s00395-014-0401-5

APA

Yaiw, K-C., Mohammad, A-A., Taher, C., Wilhelmi, V., Davoudi, B., Strååt, K., Assinger, A., Ovchinnikova, O., Shlyakhto, E., Rahbar, A., Koutonguk, O., Religa, P., Butler, L., Khan, Z., Streblow, D., Pernow, J., & Söderberg-Nauclér, C. (2014). Human cytomegalovirus induces upregulation of arginase II possible implications for vasculopathies. BASIC RES CARDIOL, 109(2), 401. https://doi.org/10.1007/s00395-014-0401-5

Vancouver

Yaiw K-C, Mohammad A-A, Taher C, Wilhelmi V, Davoudi B, Strååt K et al. Human cytomegalovirus induces upregulation of arginase II possible implications for vasculopathies. BASIC RES CARDIOL. 2014 Mar 1;109(2):401. https://doi.org/10.1007/s00395-014-0401-5

Bibtex

@article{9501653fba994bf1a4ddafd10ca35a7b,
title = "Human cytomegalovirus induces upregulation of arginase II possible implications for vasculopathies",
abstract = "Both human cytomegalovirus (HCMV) and arginase II (ARG II) have been implicated in the pathogenesis of cardiovascular diseases. The effects of HCMV on ARG II are unknown. The aim of this study was to investigate the effects of HCMV on ARG II expression in endothelial and vascular smooth muscle cells (SMC) both in vitro and ex vivo. Endothelial and SMC were infected with either HCMV or UV-irradiated HCMV. Expression of ARG II, endothelial or inducible nitric oxide synthase (eNOS and iNOS, respectively) and viral immediate early (IE) was quantified using quantitative PCR. Ganciclovir and short interfering RNA were used to determine the viral gene mediating the effects on ARG II. Detection of viral antigens and ARG II expression was performed by immunofluorescence or immunohistochemistry. HCMV infection increased both ARG II mRNA and protein levels in the examined cells; this effect was mediated by the HCMV IE2-p86 protein. The upregulation of ARG II was accompanied by a downregulation of eNOS but an induction of iNOS in HCMV-infected endothelial cells. Both eNOS and iNOS expressions were induced in HCMV-infected SMC. ARG II was abundantly expressed in endothelial cells, foam cells and SMC and was importantly significantly upregulated in HCMV-immunoreactive human carotid atherosclerotic plaques. HCMV IE2-p86 mediates ARG II upregulation in vitro and ARG II is co-expressed with HCMV antigens in human carotid atherosclerotic plaques. We speculate that HCMV may contribute to endothelial dysfunction via ARG II induction and reduced eNOS production.",
keywords = "Antiviral Agents, Aorta, Arginase, Carotid Artery Diseases, Cytomegalovirus, Cytomegalovirus Infections, Endothelial Cells, Ganciclovir, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Viral, Human Umbilical Vein Endothelial Cells, Humans, Immediate-Early Proteins, Muscle, Smooth, Vascular, Nitric Oxide Synthase Type II, Nitric Oxide Synthase Type III, Pulmonary Artery, RNA, Messenger, RNA, Small Interfering, Trans-Activators, Up-Regulation, Vasculitis",
author = "Koon-Chu Yaiw and Abdul-Aleem Mohammad and Chato Taher and Vanessa Wilhelmi and Belghis Davoudi and Klas Str{\aa}{\aa}t and Alice Assinger and Olga Ovchinnikova and Eugene Shlyakhto and Afsar Rahbar and Oksana Koutonguk and Piotr Religa and Lynn Butler and Zahidul Khan and Daniel Streblow and John Pernow and Cecilia S{\"o}derberg-Naucl{\'e}r",
year = "2014",
month = mar,
day = "1",
doi = "10.1007/s00395-014-0401-5",
language = "English",
volume = "109",
pages = "401",
journal = "BASIC RES CARDIOL",
issn = "0300-8428",
publisher = "D. Steinkopff-Verlag",
number = "2",

}

RIS

TY - JOUR

T1 - Human cytomegalovirus induces upregulation of arginase II possible implications for vasculopathies

AU - Yaiw, Koon-Chu

AU - Mohammad, Abdul-Aleem

AU - Taher, Chato

AU - Wilhelmi, Vanessa

AU - Davoudi, Belghis

AU - Strååt, Klas

AU - Assinger, Alice

AU - Ovchinnikova, Olga

AU - Shlyakhto, Eugene

AU - Rahbar, Afsar

AU - Koutonguk, Oksana

AU - Religa, Piotr

AU - Butler, Lynn

AU - Khan, Zahidul

AU - Streblow, Daniel

AU - Pernow, John

AU - Söderberg-Nauclér, Cecilia

PY - 2014/3/1

Y1 - 2014/3/1

N2 - Both human cytomegalovirus (HCMV) and arginase II (ARG II) have been implicated in the pathogenesis of cardiovascular diseases. The effects of HCMV on ARG II are unknown. The aim of this study was to investigate the effects of HCMV on ARG II expression in endothelial and vascular smooth muscle cells (SMC) both in vitro and ex vivo. Endothelial and SMC were infected with either HCMV or UV-irradiated HCMV. Expression of ARG II, endothelial or inducible nitric oxide synthase (eNOS and iNOS, respectively) and viral immediate early (IE) was quantified using quantitative PCR. Ganciclovir and short interfering RNA were used to determine the viral gene mediating the effects on ARG II. Detection of viral antigens and ARG II expression was performed by immunofluorescence or immunohistochemistry. HCMV infection increased both ARG II mRNA and protein levels in the examined cells; this effect was mediated by the HCMV IE2-p86 protein. The upregulation of ARG II was accompanied by a downregulation of eNOS but an induction of iNOS in HCMV-infected endothelial cells. Both eNOS and iNOS expressions were induced in HCMV-infected SMC. ARG II was abundantly expressed in endothelial cells, foam cells and SMC and was importantly significantly upregulated in HCMV-immunoreactive human carotid atherosclerotic plaques. HCMV IE2-p86 mediates ARG II upregulation in vitro and ARG II is co-expressed with HCMV antigens in human carotid atherosclerotic plaques. We speculate that HCMV may contribute to endothelial dysfunction via ARG II induction and reduced eNOS production.

AB - Both human cytomegalovirus (HCMV) and arginase II (ARG II) have been implicated in the pathogenesis of cardiovascular diseases. The effects of HCMV on ARG II are unknown. The aim of this study was to investigate the effects of HCMV on ARG II expression in endothelial and vascular smooth muscle cells (SMC) both in vitro and ex vivo. Endothelial and SMC were infected with either HCMV or UV-irradiated HCMV. Expression of ARG II, endothelial or inducible nitric oxide synthase (eNOS and iNOS, respectively) and viral immediate early (IE) was quantified using quantitative PCR. Ganciclovir and short interfering RNA were used to determine the viral gene mediating the effects on ARG II. Detection of viral antigens and ARG II expression was performed by immunofluorescence or immunohistochemistry. HCMV infection increased both ARG II mRNA and protein levels in the examined cells; this effect was mediated by the HCMV IE2-p86 protein. The upregulation of ARG II was accompanied by a downregulation of eNOS but an induction of iNOS in HCMV-infected endothelial cells. Both eNOS and iNOS expressions were induced in HCMV-infected SMC. ARG II was abundantly expressed in endothelial cells, foam cells and SMC and was importantly significantly upregulated in HCMV-immunoreactive human carotid atherosclerotic plaques. HCMV IE2-p86 mediates ARG II upregulation in vitro and ARG II is co-expressed with HCMV antigens in human carotid atherosclerotic plaques. We speculate that HCMV may contribute to endothelial dysfunction via ARG II induction and reduced eNOS production.

KW - Antiviral Agents

KW - Aorta

KW - Arginase

KW - Carotid Artery Diseases

KW - Cytomegalovirus

KW - Cytomegalovirus Infections

KW - Endothelial Cells

KW - Ganciclovir

KW - Gene Expression Regulation, Enzymologic

KW - Gene Expression Regulation, Viral

KW - Human Umbilical Vein Endothelial Cells

KW - Humans

KW - Immediate-Early Proteins

KW - Muscle, Smooth, Vascular

KW - Nitric Oxide Synthase Type II

KW - Nitric Oxide Synthase Type III

KW - Pulmonary Artery

KW - RNA, Messenger

KW - RNA, Small Interfering

KW - Trans-Activators

KW - Up-Regulation

KW - Vasculitis

U2 - 10.1007/s00395-014-0401-5

DO - 10.1007/s00395-014-0401-5

M3 - SCORING: Journal article

C2 - 24442486

VL - 109

SP - 401

JO - BASIC RES CARDIOL

JF - BASIC RES CARDIOL

SN - 0300-8428

IS - 2

ER -