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Human archival tissues provide a valuable source for the analysis of spatial genome organization

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Human archival tissues provide a valuable source for the analysis of spatial genome organization. / Wiech, Thorsten; Timme, Sylvia; Riede, Florian; Stein, Stefan; Schuricke, Michael; Cremer, Christoph; Werner, Martin; Hausmann, Michael; Walch, Axel.

In: HISTOCHEM CELL BIOL, Vol. 123, No. 3, 01.03.2005, p. 229-38.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wiech, T, Timme, S, Riede, F, Stein, S, Schuricke, M, Cremer, C, Werner, M, Hausmann, M & Walch, A 2005, 'Human archival tissues provide a valuable source for the analysis of spatial genome organization', HISTOCHEM CELL BIOL, vol. 123, no. 3, pp. 229-38. https://doi.org/10.1007/s00418-005-0768-3

APA

Wiech, T., Timme, S., Riede, F., Stein, S., Schuricke, M., Cremer, C., Werner, M., Hausmann, M., & Walch, A. (2005). Human archival tissues provide a valuable source for the analysis of spatial genome organization. HISTOCHEM CELL BIOL, 123(3), 229-38. https://doi.org/10.1007/s00418-005-0768-3

Vancouver

Wiech T, Timme S, Riede F, Stein S, Schuricke M, Cremer C et al. Human archival tissues provide a valuable source for the analysis of spatial genome organization. HISTOCHEM CELL BIOL. 2005 Mar 1;123(3):229-38. https://doi.org/10.1007/s00418-005-0768-3

Bibtex

@article{9b6979542fb248cbae0675eaa6da14ab,
title = "Human archival tissues provide a valuable source for the analysis of spatial genome organization",
abstract = "Sections from archival formalin-fixed, paraffin wax-embedded human tissues are a valuable source for the study of the nuclear architecture of specific tissue types in terms of the three-dimensional spatial positioning and architecture of chromosome territories and sub-chromosomal domains. Chromosome painting, centromeric, and locus-specific probes were hybridized to tissue microarrays prepared from formalin-fixed paraffin wax-embedded samples of pancreas and breast. The cell nuclei were analyzed using quantitative three-dimensional image microscopy. The results obtained from non-neoplastic pancreatic cells of randomly selected individuals indicated that the radial arrangement of the chromosome 8 territories as well as their shape (roundness) did not significantly differ between the individuals and were in accordance with assumptions of a probabilistic model for computer simulations. There were considerable differences between pancreatic tumor and non-neoplastic cells. In non-neoplastic ductal epithelium of the breast there was a larger, but insignificant, variability in the three-dimensional positioning of the centromere 17 and HER2 domains between individuals. In neoplastic epithelial breast cells, however, the distances between centromere and gene domains were, on average, smaller than in non-neoplastic cells. In conclusion, our results demonstrate the feasibility of studying the genome architecture in archival, formalin-fixed, paraffin wax-embedded human tissues, opening new directions in tumor research and cell classification.",
keywords = "Breast, Breast Neoplasms, Carcinoma, Ductal, Breast, Carcinoma, Pancreatic Ductal, Chromosome Positioning, Epithelium, Genome, Human, Humans, In Situ Hybridization, Fluorescence, Pancreatic Ducts, Pancreatic Neoplasms, Tissue Fixation",
author = "Thorsten Wiech and Sylvia Timme and Florian Riede and Stefan Stein and Michael Schuricke and Christoph Cremer and Martin Werner and Michael Hausmann and Axel Walch",
year = "2005",
month = mar,
day = "1",
doi = "10.1007/s00418-005-0768-3",
language = "English",
volume = "123",
pages = "229--38",
journal = "HISTOCHEM CELL BIOL",
issn = "0948-6143",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - Human archival tissues provide a valuable source for the analysis of spatial genome organization

AU - Wiech, Thorsten

AU - Timme, Sylvia

AU - Riede, Florian

AU - Stein, Stefan

AU - Schuricke, Michael

AU - Cremer, Christoph

AU - Werner, Martin

AU - Hausmann, Michael

AU - Walch, Axel

PY - 2005/3/1

Y1 - 2005/3/1

N2 - Sections from archival formalin-fixed, paraffin wax-embedded human tissues are a valuable source for the study of the nuclear architecture of specific tissue types in terms of the three-dimensional spatial positioning and architecture of chromosome territories and sub-chromosomal domains. Chromosome painting, centromeric, and locus-specific probes were hybridized to tissue microarrays prepared from formalin-fixed paraffin wax-embedded samples of pancreas and breast. The cell nuclei were analyzed using quantitative three-dimensional image microscopy. The results obtained from non-neoplastic pancreatic cells of randomly selected individuals indicated that the radial arrangement of the chromosome 8 territories as well as their shape (roundness) did not significantly differ between the individuals and were in accordance with assumptions of a probabilistic model for computer simulations. There were considerable differences between pancreatic tumor and non-neoplastic cells. In non-neoplastic ductal epithelium of the breast there was a larger, but insignificant, variability in the three-dimensional positioning of the centromere 17 and HER2 domains between individuals. In neoplastic epithelial breast cells, however, the distances between centromere and gene domains were, on average, smaller than in non-neoplastic cells. In conclusion, our results demonstrate the feasibility of studying the genome architecture in archival, formalin-fixed, paraffin wax-embedded human tissues, opening new directions in tumor research and cell classification.

AB - Sections from archival formalin-fixed, paraffin wax-embedded human tissues are a valuable source for the study of the nuclear architecture of specific tissue types in terms of the three-dimensional spatial positioning and architecture of chromosome territories and sub-chromosomal domains. Chromosome painting, centromeric, and locus-specific probes were hybridized to tissue microarrays prepared from formalin-fixed paraffin wax-embedded samples of pancreas and breast. The cell nuclei were analyzed using quantitative three-dimensional image microscopy. The results obtained from non-neoplastic pancreatic cells of randomly selected individuals indicated that the radial arrangement of the chromosome 8 territories as well as their shape (roundness) did not significantly differ between the individuals and were in accordance with assumptions of a probabilistic model for computer simulations. There were considerable differences between pancreatic tumor and non-neoplastic cells. In non-neoplastic ductal epithelium of the breast there was a larger, but insignificant, variability in the three-dimensional positioning of the centromere 17 and HER2 domains between individuals. In neoplastic epithelial breast cells, however, the distances between centromere and gene domains were, on average, smaller than in non-neoplastic cells. In conclusion, our results demonstrate the feasibility of studying the genome architecture in archival, formalin-fixed, paraffin wax-embedded human tissues, opening new directions in tumor research and cell classification.

KW - Breast

KW - Breast Neoplasms

KW - Carcinoma, Ductal, Breast

KW - Carcinoma, Pancreatic Ductal

KW - Chromosome Positioning

KW - Epithelium

KW - Genome, Human

KW - Humans

KW - In Situ Hybridization, Fluorescence

KW - Pancreatic Ducts

KW - Pancreatic Neoplasms

KW - Tissue Fixation

U2 - 10.1007/s00418-005-0768-3

DO - 10.1007/s00418-005-0768-3

M3 - SCORING: Journal article

C2 - 15827756

VL - 123

SP - 229

EP - 238

JO - HISTOCHEM CELL BIOL

JF - HISTOCHEM CELL BIOL

SN - 0948-6143

IS - 3

ER -