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HPV DNA, E6*I-mRNA expression and p16INK4A immunohistochemistry in head and neck cancer - how valid is p16INK4A as surrogate marker?

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HPV DNA, E6*I-mRNA expression and p16INK4A immunohistochemistry in head and neck cancer - how valid is p16INK4A as surrogate marker? / Hoffmann, Markus; Tribius, Silke; Quabius, Elgar Susanne; Henry, Hannes; Pfannenschmidt, Saskia; Burkhardt, Claudia; Görögh, Tibor; Halec, Gordana; Hoffmann, Anna Sophie; Kahn, Tomas; Röcken, Christoph; Haag, Jochen; Waterboer, Tim; Schmitt, Markus.

In: CANCER LETT, Vol. 323, No. 1, 1, 2012, p. 88-96.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hoffmann, M, Tribius, S, Quabius, ES, Henry, H, Pfannenschmidt, S, Burkhardt, C, Görögh, T, Halec, G, Hoffmann, AS, Kahn, T, Röcken, C, Haag, J, Waterboer, T & Schmitt, M 2012, 'HPV DNA, E6*I-mRNA expression and p16INK4A immunohistochemistry in head and neck cancer - how valid is p16INK4A as surrogate marker?', CANCER LETT, vol. 323, no. 1, 1, pp. 88-96. <http://www.ncbi.nlm.nih.gov/pubmed/22484467?dopt=Citation>

APA

Hoffmann, M., Tribius, S., Quabius, E. S., Henry, H., Pfannenschmidt, S., Burkhardt, C., Görögh, T., Halec, G., Hoffmann, A. S., Kahn, T., Röcken, C., Haag, J., Waterboer, T., & Schmitt, M. (2012). HPV DNA, E6*I-mRNA expression and p16INK4A immunohistochemistry in head and neck cancer - how valid is p16INK4A as surrogate marker? CANCER LETT, 323(1), 88-96. [1]. http://www.ncbi.nlm.nih.gov/pubmed/22484467?dopt=Citation

Vancouver

Hoffmann M, Tribius S, Quabius ES, Henry H, Pfannenschmidt S, Burkhardt C et al. HPV DNA, E6*I-mRNA expression and p16INK4A immunohistochemistry in head and neck cancer - how valid is p16INK4A as surrogate marker? CANCER LETT. 2012;323(1):88-96. 1.

Bibtex

@article{27d3f6d3a1b74b8285de7f8aac6584b3,
title = "HPV DNA, E6*I-mRNA expression and p16INK4A immunohistochemistry in head and neck cancer - how valid is p16INK4A as surrogate marker?",
abstract = "It has been proposed that p16(INK4A) qualifies as a surrogate marker for viral oncogene activity in head and neck cancer (HNSCC). By analyzing 78 HNSCC we sought to validate the accuracy of p16(INK4A) as a reliable marker of active HPV infections in HNSCC. To this end we determined HPV DNA (HPVD) and E6*I mRNA (HPVR) expression status and correlated these results with p16(INK4A) staining. In tonsillar SCC 12/20 were HPVD+ and 12/12 of these showed active HPV infections whereas in non-tonsillar SCC 10/58 were HPVD+ and 5/10 showed active HPV infections. Thus, we prove about 8% of non-tonsillar SCC to be also correlated with HPV-associated carcinogenesis. Strikingly, 3/14 (21.4%) of tonsillar and non-tonsillar HPVD+/HPVR+ cases did not show p16(INK4A) overexpression and these cases would have been missed when applying initial p16(INK4A) staining only. However, in 13 cases negative for HPV, DNA p16(INK4A) was overexpressed. In conclusion, our data confirm tonsillar SCC to be predominantly but not only associated with active HPV infections. Furthermore, our data show that p16(INK4A) overexpression is not evident in a subgroup of HNSCC with active HPV infection. Definitive HPV data should therefore be utilized in diagnostics and treatment modalities of HPV positive and HPV negative HNSCC patients, resulting in a paradigm shift regarding these obviously different tumor entities.",
keywords = "Adult, Humans, Male, Aged, Female, Middle Aged, Immunohistochemistry, RNA, Messenger/analysis, Biological Markers/*analysis, Carcinoma, Squamous Cell/metabolism/*virology, Cyclin-Dependent Kinase Inhibitor p16/*analysis, DNA, Viral/*analysis, Head and Neck Neoplasms/metabolism/*virology, Multiplex Polymerase Chain Reaction, Oncogene Proteins, Viral/*analysis, Papillomavirus Infections/complications/diagnosis, Tonsillar Neoplasms/metabolism/virology, Adult, Humans, Male, Aged, Female, Middle Aged, Immunohistochemistry, RNA, Messenger/analysis, Biological Markers/*analysis, Carcinoma, Squamous Cell/metabolism/*virology, Cyclin-Dependent Kinase Inhibitor p16/*analysis, DNA, Viral/*analysis, Head and Neck Neoplasms/metabolism/*virology, Multiplex Polymerase Chain Reaction, Oncogene Proteins, Viral/*analysis, Papillomavirus Infections/complications/diagnosis, Tonsillar Neoplasms/metabolism/virology",
author = "Markus Hoffmann and Silke Tribius and Quabius, {Elgar Susanne} and Hannes Henry and Saskia Pfannenschmidt and Claudia Burkhardt and Tibor G{\"o}r{\"o}gh and Gordana Halec and Hoffmann, {Anna Sophie} and Tomas Kahn and Christoph R{\"o}cken and Jochen Haag and Tim Waterboer and Markus Schmitt",
year = "2012",
language = "English",
volume = "323",
pages = "88--96",
journal = "CANCER LETT",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - HPV DNA, E6*I-mRNA expression and p16INK4A immunohistochemistry in head and neck cancer - how valid is p16INK4A as surrogate marker?

AU - Hoffmann, Markus

AU - Tribius, Silke

AU - Quabius, Elgar Susanne

AU - Henry, Hannes

AU - Pfannenschmidt, Saskia

AU - Burkhardt, Claudia

AU - Görögh, Tibor

AU - Halec, Gordana

AU - Hoffmann, Anna Sophie

AU - Kahn, Tomas

AU - Röcken, Christoph

AU - Haag, Jochen

AU - Waterboer, Tim

AU - Schmitt, Markus

PY - 2012

Y1 - 2012

N2 - It has been proposed that p16(INK4A) qualifies as a surrogate marker for viral oncogene activity in head and neck cancer (HNSCC). By analyzing 78 HNSCC we sought to validate the accuracy of p16(INK4A) as a reliable marker of active HPV infections in HNSCC. To this end we determined HPV DNA (HPVD) and E6*I mRNA (HPVR) expression status and correlated these results with p16(INK4A) staining. In tonsillar SCC 12/20 were HPVD+ and 12/12 of these showed active HPV infections whereas in non-tonsillar SCC 10/58 were HPVD+ and 5/10 showed active HPV infections. Thus, we prove about 8% of non-tonsillar SCC to be also correlated with HPV-associated carcinogenesis. Strikingly, 3/14 (21.4%) of tonsillar and non-tonsillar HPVD+/HPVR+ cases did not show p16(INK4A) overexpression and these cases would have been missed when applying initial p16(INK4A) staining only. However, in 13 cases negative for HPV, DNA p16(INK4A) was overexpressed. In conclusion, our data confirm tonsillar SCC to be predominantly but not only associated with active HPV infections. Furthermore, our data show that p16(INK4A) overexpression is not evident in a subgroup of HNSCC with active HPV infection. Definitive HPV data should therefore be utilized in diagnostics and treatment modalities of HPV positive and HPV negative HNSCC patients, resulting in a paradigm shift regarding these obviously different tumor entities.

AB - It has been proposed that p16(INK4A) qualifies as a surrogate marker for viral oncogene activity in head and neck cancer (HNSCC). By analyzing 78 HNSCC we sought to validate the accuracy of p16(INK4A) as a reliable marker of active HPV infections in HNSCC. To this end we determined HPV DNA (HPVD) and E6*I mRNA (HPVR) expression status and correlated these results with p16(INK4A) staining. In tonsillar SCC 12/20 were HPVD+ and 12/12 of these showed active HPV infections whereas in non-tonsillar SCC 10/58 were HPVD+ and 5/10 showed active HPV infections. Thus, we prove about 8% of non-tonsillar SCC to be also correlated with HPV-associated carcinogenesis. Strikingly, 3/14 (21.4%) of tonsillar and non-tonsillar HPVD+/HPVR+ cases did not show p16(INK4A) overexpression and these cases would have been missed when applying initial p16(INK4A) staining only. However, in 13 cases negative for HPV, DNA p16(INK4A) was overexpressed. In conclusion, our data confirm tonsillar SCC to be predominantly but not only associated with active HPV infections. Furthermore, our data show that p16(INK4A) overexpression is not evident in a subgroup of HNSCC with active HPV infection. Definitive HPV data should therefore be utilized in diagnostics and treatment modalities of HPV positive and HPV negative HNSCC patients, resulting in a paradigm shift regarding these obviously different tumor entities.

KW - Adult

KW - Humans

KW - Male

KW - Aged

KW - Female

KW - Middle Aged

KW - Immunohistochemistry

KW - RNA, Messenger/analysis

KW - Biological Markers/analysis

KW - Carcinoma, Squamous Cell/metabolism/virology

KW - Cyclin-Dependent Kinase Inhibitor p16/analysis

KW - DNA, Viral/analysis

KW - Head and Neck Neoplasms/metabolism/virology

KW - Multiplex Polymerase Chain Reaction

KW - Oncogene Proteins, Viral/analysis

KW - Papillomavirus Infections/complications/diagnosis

KW - Tonsillar Neoplasms/metabolism/virology

KW - Adult

KW - Humans

KW - Male

KW - Aged

KW - Female

KW - Middle Aged

KW - Immunohistochemistry

KW - RNA, Messenger/analysis

KW - Biological Markers/analysis

KW - Carcinoma, Squamous Cell/metabolism/virology

KW - Cyclin-Dependent Kinase Inhibitor p16/analysis

KW - DNA, Viral/analysis

KW - Head and Neck Neoplasms/metabolism/virology

KW - Multiplex Polymerase Chain Reaction

KW - Oncogene Proteins, Viral/analysis

KW - Papillomavirus Infections/complications/diagnosis

KW - Tonsillar Neoplasms/metabolism/virology

M3 - SCORING: Journal article

VL - 323

SP - 88

EP - 96

JO - CANCER LETT

JF - CANCER LETT

SN - 0304-3835

IS - 1

M1 - 1

ER -