How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology
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How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology. / Wu, You; Sun, Jiangling; Wang, Wang; Wang, Yao; Friedrich, Reinhard E.
In: FRONT BIOENG BIOTECH, Vol. 12, 2024, p. 1324049.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology
AU - Wu, You
AU - Sun, Jiangling
AU - Wang, Wang
AU - Wang, Yao
AU - Friedrich, Reinhard E
N1 - Copyright © 2024 Wu, Sun, Wang, Wang and Friedrich.
PY - 2024
Y1 - 2024
N2 - INTRODUCTION: Dental pulp stem cells from humans possess self-renewal and versatile differentiation abilities. These cells, known as DPSC, are promising for tissue engineering due to their outstanding biological characteristics and ease of access without significant donor site trauma. Existing methods for isolating DPSC mainly include enzyme digestion and explant techniques. Compared with the enzymatic digestion technique, the outgrowth method is less prone to cell damage and loss during the operation, which is essential for DPSC with fewer tissue sources.METHODS: In order to maximize the amount of stem cells harvested while reducing the cost of DPSC culture, the feasibility of the optimized explant technique was evaluated in this experiment. Cell morphology, minimum cell emergence time, the total amount of cells harvested, cell survival, and proliferative and differentiation capacity of DPSC obtained with different numbers of explant attachments (A1-A5) were evaluated.RESULTS: There was a reduction in the survival rate of the cells in groups A2-A5, and the amount of harvested DPSC decreased in A3-A5 groups, but the DPSC harvested in groups A1-A4 had similar proliferative and differentiation abilities. However, starting from group A5, the survival rate, proliferation and differentiation ability of DPSC decreased significantly, and the adipogenic trend of the cells became more apparent, indicating that the cells had begun to enter the senescence state.DISCUSSION: The results of our study demonstrated that the DPSC obtained by the optimized explant method up to 4 times had reliable biological properties and is available for tissue engineering.
AB - INTRODUCTION: Dental pulp stem cells from humans possess self-renewal and versatile differentiation abilities. These cells, known as DPSC, are promising for tissue engineering due to their outstanding biological characteristics and ease of access without significant donor site trauma. Existing methods for isolating DPSC mainly include enzyme digestion and explant techniques. Compared with the enzymatic digestion technique, the outgrowth method is less prone to cell damage and loss during the operation, which is essential for DPSC with fewer tissue sources.METHODS: In order to maximize the amount of stem cells harvested while reducing the cost of DPSC culture, the feasibility of the optimized explant technique was evaluated in this experiment. Cell morphology, minimum cell emergence time, the total amount of cells harvested, cell survival, and proliferative and differentiation capacity of DPSC obtained with different numbers of explant attachments (A1-A5) were evaluated.RESULTS: There was a reduction in the survival rate of the cells in groups A2-A5, and the amount of harvested DPSC decreased in A3-A5 groups, but the DPSC harvested in groups A1-A4 had similar proliferative and differentiation abilities. However, starting from group A5, the survival rate, proliferation and differentiation ability of DPSC decreased significantly, and the adipogenic trend of the cells became more apparent, indicating that the cells had begun to enter the senescence state.DISCUSSION: The results of our study demonstrated that the DPSC obtained by the optimized explant method up to 4 times had reliable biological properties and is available for tissue engineering.
U2 - 10.3389/fbioe.2024.1324049
DO - 10.3389/fbioe.2024.1324049
M3 - SCORING: Journal article
C2 - 38562666
VL - 12
SP - 1324049
JO - FRONT BIOENG BIOTECH
JF - FRONT BIOENG BIOTECH
SN - 2296-4185
ER -
