HLA-E contributes to an immune-inhibitory phenotype of glioblastoma stem-like cells

Fabian Wolpert; Patrick Roth; Katrin Lamszus; Ghazaleh Tabatabai; Michael Weller; Günter Eisele

doi:10.1016/j.jneuroim.2012.05.010

HLA-E contributes to an immune-inhibitory phenotype of glioblastoma stem-like cells

Standard

HLA-E contributes to an immune-inhibitory phenotype of glioblastoma stem-like cells. / Wolpert, Fabian; Roth, Patrick; Lamszus, Katrin; Tabatabai, Ghazaleh; Weller, Michael; Eisele, Günter.

In: J NEUROIMMUNOL, Vol. 250, No. 1-2, 15.09.2012, p. 27-34.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Wolpert, F, Roth, P, Lamszus, K, Tabatabai, G, Weller, M & Eisele, G 2012, 'HLA-E contributes to an immune-inhibitory phenotype of glioblastoma stem-like cells', J NEUROIMMUNOL, vol. 250, no. 1-2, pp. 27-34. https://doi.org/10.1016/j.jneuroim.2012.05.010

APA

Wolpert, F., Roth, P., Lamszus, K., Tabatabai, G., Weller, M., & Eisele, G. (2012). HLA-E contributes to an immune-inhibitory phenotype of glioblastoma stem-like cells. J NEUROIMMUNOL, 250(1-2), 27-34. https://doi.org/10.1016/j.jneuroim.2012.05.010

Vancouver

Wolpert F, Roth P, Lamszus K, Tabatabai G, Weller M, Eisele G. HLA-E contributes to an immune-inhibitory phenotype of glioblastoma stem-like cells. J NEUROIMMUNOL. 2012 Sep 15;250(1-2):27-34. https://doi.org/10.1016/j.jneuroim.2012.05.010

Bibtex

@article{2f18ea8d09294b708f70ad306aeee2b8,

title = "HLA-E contributes to an immune-inhibitory phenotype of glioblastoma stem-like cells",

abstract = "Cancer stem cells are an attractive target for immunotherapeutic approaches to glioblastoma. However, an immune inhibitory phenotype of cells currently classified as {"}glioma-initiating cells{"} (GIC) might counteract recognition by immune effector cells. Here, we investigate the contribution of the non-classical MHC molecule HLA-E to the immunosuppressive phenotype of GIC. HLA-E is expressed in GIC lines and its expression is reduced upon differentiation of GIC in serum-containing culture conditions. Constitutive HLA-E inhibits natural killer (NK) cell-mediated lysis of GIC since small-interfering RNA-mediated HLA-E gene silencing enhances the immunogenicity of GIC. Increased GIC lysis was observed both in the CD133+ and in the CD133- compartment. Furthermore, the use of interferon-γ as a possible agent to boost an immune response against glioblastoma cells might be limited by the concurrent upregulation of HLA-E.",

keywords = "Brain Neoplasms, Cell Line, Tumor, Flow Cytometry, Glioblastoma, Histocompatibility Antigens Class I, Humans, Killer Cells, Natural, Neoplastic Stem Cells, Phenotype, Real-Time Polymerase Chain Reaction",

author = "Fabian Wolpert and Patrick Roth and Katrin Lamszus and Ghazaleh Tabatabai and Michael Weller and G{\"u}nter Eisele",

year = "2012",

month = sep,

day = "15",

doi = "10.1016/j.jneuroim.2012.05.010",

language = "English",

volume = "250",

pages = "27--34",

journal = "J NEUROIMMUNOL",

issn = "0165-5728",

publisher = "Elsevier",

number = "1-2",

}

RIS

TY - JOUR

T1 - HLA-E contributes to an immune-inhibitory phenotype of glioblastoma stem-like cells

AU - Wolpert, Fabian

AU - Roth, Patrick

AU - Lamszus, Katrin

AU - Tabatabai, Ghazaleh

AU - Weller, Michael

AU - Eisele, Günter

PY - 2012/9/15

Y1 - 2012/9/15

N2 - Cancer stem cells are an attractive target for immunotherapeutic approaches to glioblastoma. However, an immune inhibitory phenotype of cells currently classified as "glioma-initiating cells" (GIC) might counteract recognition by immune effector cells. Here, we investigate the contribution of the non-classical MHC molecule HLA-E to the immunosuppressive phenotype of GIC. HLA-E is expressed in GIC lines and its expression is reduced upon differentiation of GIC in serum-containing culture conditions. Constitutive HLA-E inhibits natural killer (NK) cell-mediated lysis of GIC since small-interfering RNA-mediated HLA-E gene silencing enhances the immunogenicity of GIC. Increased GIC lysis was observed both in the CD133+ and in the CD133- compartment. Furthermore, the use of interferon-γ as a possible agent to boost an immune response against glioblastoma cells might be limited by the concurrent upregulation of HLA-E.

AB - Cancer stem cells are an attractive target for immunotherapeutic approaches to glioblastoma. However, an immune inhibitory phenotype of cells currently classified as "glioma-initiating cells" (GIC) might counteract recognition by immune effector cells. Here, we investigate the contribution of the non-classical MHC molecule HLA-E to the immunosuppressive phenotype of GIC. HLA-E is expressed in GIC lines and its expression is reduced upon differentiation of GIC in serum-containing culture conditions. Constitutive HLA-E inhibits natural killer (NK) cell-mediated lysis of GIC since small-interfering RNA-mediated HLA-E gene silencing enhances the immunogenicity of GIC. Increased GIC lysis was observed both in the CD133+ and in the CD133- compartment. Furthermore, the use of interferon-γ as a possible agent to boost an immune response against glioblastoma cells might be limited by the concurrent upregulation of HLA-E.

KW - Brain Neoplasms

KW - Cell Line, Tumor

KW - Flow Cytometry

KW - Glioblastoma

KW - Histocompatibility Antigens Class I

KW - Humans

KW - Killer Cells, Natural

KW - Neoplastic Stem Cells

KW - Phenotype

KW - Real-Time Polymerase Chain Reaction

U2 - 10.1016/j.jneuroim.2012.05.010

DO - 10.1016/j.jneuroim.2012.05.010

M3 - SCORING: Journal article

C2 - 22688424

VL - 250

SP - 27

EP - 34

JO - J NEUROIMMUNOL

JF - J NEUROIMMUNOL

SN - 0165-5728

IS - 1-2

ER -