High-Resolution Calcium Imaging Method for Local Calcium Signaling
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High-Resolution Calcium Imaging Method for Local Calcium Signaling. / Diercks, Björn-Philipp; Werner, Rene; Schetelig, Daniel; Wolf, Insa; Guse, Andreas.
Calcium-Binding Proteins of the EF-Hand Superfamily. Vol. 1929 1. ed. Humana Press, 2019. p. 27-39.Research output: SCORING: Contribution to book/anthology › SCORING: Contribution to collected editions/anthologies › Research › peer-review
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TY - CHAP
T1 - High-Resolution Calcium Imaging Method for Local Calcium Signaling
AU - Diercks, Björn-Philipp
AU - Werner, Rene
AU - Schetelig, Daniel
AU - Wolf, Insa
AU - Guse, Andreas
PY - 2019
Y1 - 2019
N2 - All eukaryotic cells respond to extracellular signals in a physiologically meaningful way. For multicellular organisms, physiologically relevant cooperation is only possible, if cell-cell communication works properly. Here, the extracellular signals must be translated into intracellular signals that ultimately result in cellular responses. This process is termed signal transduction or signaling. Ca2+ signaling has been developed in almost all eukaryotic cells. The cellular components used for this highly versatile signaling system are often termed “Ca2+ toolbox”. Besides Ca2+ pumps and Ca2+-binding proteins, the Ca2+ channels that are located in the plasma membrane and intracellular membranes and the Ca2+-mobilizing second messengers are major players in shaping the four-dimensional nature of Ca2+ signals. Here, we report on methodological developments to acquire and analyze cellular Ca2+ signals with high temporal and spatial resolution with specific focus on (1) photobleaching of Ca2+ indicators at high acquisition rate, (2) determination of system noise and spatiotemporal detection limits, and (3) image processing.
AB - All eukaryotic cells respond to extracellular signals in a physiologically meaningful way. For multicellular organisms, physiologically relevant cooperation is only possible, if cell-cell communication works properly. Here, the extracellular signals must be translated into intracellular signals that ultimately result in cellular responses. This process is termed signal transduction or signaling. Ca2+ signaling has been developed in almost all eukaryotic cells. The cellular components used for this highly versatile signaling system are often termed “Ca2+ toolbox”. Besides Ca2+ pumps and Ca2+-binding proteins, the Ca2+ channels that are located in the plasma membrane and intracellular membranes and the Ca2+-mobilizing second messengers are major players in shaping the four-dimensional nature of Ca2+ signals. Here, we report on methodological developments to acquire and analyze cellular Ca2+ signals with high temporal and spatial resolution with specific focus on (1) photobleaching of Ca2+ indicators at high acquisition rate, (2) determination of system noise and spatiotemporal detection limits, and (3) image processing.
M3 - SCORING: Contribution to collected editions/anthologies
SN - 978-1-4939-9029-0
VL - 1929
SP - 27
EP - 39
BT - Calcium-Binding Proteins of the EF-Hand Superfamily
PB - Humana Press
ER -