High-resolution analyses of copy number changes in disseminated tumor cells of patients with breast cancer.
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High-resolution analyses of copy number changes in disseminated tumor cells of patients with breast cancer. / Mathiesen, Randi R; Fjelldal, Renathe; Liestøl, Knut; Due, Eldri U; Geigl, Jochen B; Riethdorf, Sabine; Borgen, Elin; Rye, Inga H; Schneider, Ida J; Obenauf, Anna C; Mauermann, Oliver; Nilsen, Gro; Ole, Christian Lingjaerde; Børresen-Dale, Anne-Lise; Pantel, Klaus; Speicher, Michael R; Naume, Bjørn; Baumbusch, Lars O.
In: INT J CANCER, Vol. 131, No. 4, 4, 2012, p. 405-415.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - High-resolution analyses of copy number changes in disseminated tumor cells of patients with breast cancer.
AU - Mathiesen, Randi R
AU - Fjelldal, Renathe
AU - Liestøl, Knut
AU - Due, Eldri U
AU - Geigl, Jochen B
AU - Riethdorf, Sabine
AU - Borgen, Elin
AU - Rye, Inga H
AU - Schneider, Ida J
AU - Obenauf, Anna C
AU - Mauermann, Oliver
AU - Nilsen, Gro
AU - Ole, Christian Lingjaerde
AU - Børresen-Dale, Anne-Lise
AU - Pantel, Klaus
AU - Speicher, Michael R
AU - Naume, Bjørn
AU - Baumbusch, Lars O
PY - 2012
Y1 - 2012
N2 - The presence of disseminated tumor cells (DTCs) in bone marrow (BM) identifies breast cancer patients with less favorable outcome. Furthermore, molecular characterization is required to investigate the malignant potential of these cells. This study presents a single-cell array comparative genomic hybridization (SCaCGH) method providing molecular analysis of immunomorphologically detected DTCs. The resolution limit of the method was estimated using the cancer cell line SK-BR-3 on 44 and 244k arrays. The technique was further tested on 28 circulating tumor cells and four hematopoietic cells (HCs) from peripheral blood (n = 8 patients). The SCaCGH method was finally applied to 24 DTCs, three immunopositive cells morphologically classified as probable HCs from breast cancer patients and five HC controls from BM (n = 7 patients plus n = 1 healthy donor). The frequency of copy number changes of the DTCs revealed similarities with primary breast tumor samples. Three of the patients had available profiles for DTCs and the corresponding tumor tissue from primary surgery. More than two-third of the analyzed DTCs disclosed equivalent changes, both to each other and to the corresponding primary disease, whereas the rest of the cells showed balanced profiles. The probable HCs revealed either balanced profiles (n = 2) or changes comparable to the tumor tissue and DTCs (n = 1), indicating morphological overlap between HCs and DTCs. Similar aberration patterns were visible in DTCs collected at diagnosis and at 3 years relapse-free follow-up. SCaCGH may be a powerful tool for the molecular characterization of DTCs.
AB - The presence of disseminated tumor cells (DTCs) in bone marrow (BM) identifies breast cancer patients with less favorable outcome. Furthermore, molecular characterization is required to investigate the malignant potential of these cells. This study presents a single-cell array comparative genomic hybridization (SCaCGH) method providing molecular analysis of immunomorphologically detected DTCs. The resolution limit of the method was estimated using the cancer cell line SK-BR-3 on 44 and 244k arrays. The technique was further tested on 28 circulating tumor cells and four hematopoietic cells (HCs) from peripheral blood (n = 8 patients). The SCaCGH method was finally applied to 24 DTCs, three immunopositive cells morphologically classified as probable HCs from breast cancer patients and five HC controls from BM (n = 7 patients plus n = 1 healthy donor). The frequency of copy number changes of the DTCs revealed similarities with primary breast tumor samples. Three of the patients had available profiles for DTCs and the corresponding tumor tissue from primary surgery. More than two-third of the analyzed DTCs disclosed equivalent changes, both to each other and to the corresponding primary disease, whereas the rest of the cells showed balanced profiles. The probable HCs revealed either balanced profiles (n = 2) or changes comparable to the tumor tissue and DTCs (n = 1), indicating morphological overlap between HCs and DTCs. Similar aberration patterns were visible in DTCs collected at diagnosis and at 3 years relapse-free follow-up. SCaCGH may be a powerful tool for the molecular characterization of DTCs.
M3 - SCORING: Journal article
VL - 131
SP - 405
EP - 415
JO - INT J CANCER
JF - INT J CANCER
SN - 0020-7136
IS - 4
M1 - 4
ER -