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Highly-efficient gene transfer with retroviral vectors into human T lymphocytes on fibronectin

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Highly-efficient gene transfer with retroviral vectors into human T lymphocytes on fibronectin. / Fehse, B; Schade, U M; Li, Z; Uhde, A; Koch, S; Goller, B; Rüger, R; Fehse, N; Stockschläder, M; Zander, A R.

In: BRIT J HAEMATOL, Vol. 102, No. 2, 01.07.1998, p. 566-74.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Fehse, B, Schade, UM, Li, Z, Uhde, A, Koch, S, Goller, B, Rüger, R, Fehse, N, Stockschläder, M & Zander, AR 1998, 'Highly-efficient gene transfer with retroviral vectors into human T lymphocytes on fibronectin', BRIT J HAEMATOL, vol. 102, no. 2, pp. 566-74.

APA

Fehse, B., Schade, U. M., Li, Z., Uhde, A., Koch, S., Goller, B., Rüger, R., Fehse, N., Stockschläder, M., & Zander, A. R. (1998). Highly-efficient gene transfer with retroviral vectors into human T lymphocytes on fibronectin. BRIT J HAEMATOL, 102(2), 566-74.

Vancouver

Fehse B, Schade UM, Li Z, Uhde A, Koch S, Goller B et al. Highly-efficient gene transfer with retroviral vectors into human T lymphocytes on fibronectin. BRIT J HAEMATOL. 1998 Jul 1;102(2):566-74.

Bibtex

@article{fbf17cf40e5f40d79c551dee0d5e038c,
title = "Highly-efficient gene transfer with retroviral vectors into human T lymphocytes on fibronectin",
abstract = "Genetically modified lymphocytes have been successfully used for correction of ADA deficiency in children and in controlling graft-versus-host disease (GvHD) after allogeneic bone marrow transplantation. Low transduction efficiencies are, however, limiting for gene therapeutic strategies based on lymphocytes. In this study we compared protocols for highly efficient gene transfer into human T cells using retroviral vector-containing supernatant. We showed that infection of both human primary T cells and CD4+ Jurkat cells is most efficient on the matrix component fibronectin. Transduction was carried out with a retroviral vector encoding both the human intracytoplasmatically truncated low-affinity nerve growth factor receptor (deltaLNGFR) as a gene transfer marker and the Herpes simplex virus thymidine kinase for negative selection. Based on LNGFR expression genetically modified cells were enriched to near purity by magnetic cell sorting (MACS). Enriched cells could be shown to be highly sensitive to ganciclovir.",
keywords = "Anti-Allergic Agents, Fibronectins, Ganciclovir, Gene Transfer Techniques, Genetic Vectors, Humans, Integrin alpha4beta1, Integrins, Jurkat Cells, Receptors, Fibronectin, Receptors, Lymphocyte Homing, Receptors, Nerve Growth Factor, Retroviridae, T-Lymphocytes, Thymidine Kinase",
author = "B Fehse and Schade, {U M} and Z Li and A Uhde and S Koch and B Goller and R R{\"u}ger and N Fehse and M Stockschl{\"a}der and Zander, {A R}",
year = "1998",
month = jul,
day = "1",
language = "English",
volume = "102",
pages = "566--74",
journal = "BRIT J HAEMATOL",
issn = "0007-1048",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Highly-efficient gene transfer with retroviral vectors into human T lymphocytes on fibronectin

AU - Fehse, B

AU - Schade, U M

AU - Li, Z

AU - Uhde, A

AU - Koch, S

AU - Goller, B

AU - Rüger, R

AU - Fehse, N

AU - Stockschläder, M

AU - Zander, A R

PY - 1998/7/1

Y1 - 1998/7/1

N2 - Genetically modified lymphocytes have been successfully used for correction of ADA deficiency in children and in controlling graft-versus-host disease (GvHD) after allogeneic bone marrow transplantation. Low transduction efficiencies are, however, limiting for gene therapeutic strategies based on lymphocytes. In this study we compared protocols for highly efficient gene transfer into human T cells using retroviral vector-containing supernatant. We showed that infection of both human primary T cells and CD4+ Jurkat cells is most efficient on the matrix component fibronectin. Transduction was carried out with a retroviral vector encoding both the human intracytoplasmatically truncated low-affinity nerve growth factor receptor (deltaLNGFR) as a gene transfer marker and the Herpes simplex virus thymidine kinase for negative selection. Based on LNGFR expression genetically modified cells were enriched to near purity by magnetic cell sorting (MACS). Enriched cells could be shown to be highly sensitive to ganciclovir.

AB - Genetically modified lymphocytes have been successfully used for correction of ADA deficiency in children and in controlling graft-versus-host disease (GvHD) after allogeneic bone marrow transplantation. Low transduction efficiencies are, however, limiting for gene therapeutic strategies based on lymphocytes. In this study we compared protocols for highly efficient gene transfer into human T cells using retroviral vector-containing supernatant. We showed that infection of both human primary T cells and CD4+ Jurkat cells is most efficient on the matrix component fibronectin. Transduction was carried out with a retroviral vector encoding both the human intracytoplasmatically truncated low-affinity nerve growth factor receptor (deltaLNGFR) as a gene transfer marker and the Herpes simplex virus thymidine kinase for negative selection. Based on LNGFR expression genetically modified cells were enriched to near purity by magnetic cell sorting (MACS). Enriched cells could be shown to be highly sensitive to ganciclovir.

KW - Anti-Allergic Agents

KW - Fibronectins

KW - Ganciclovir

KW - Gene Transfer Techniques

KW - Genetic Vectors

KW - Humans

KW - Integrin alpha4beta1

KW - Integrins

KW - Jurkat Cells

KW - Receptors, Fibronectin

KW - Receptors, Lymphocyte Homing

KW - Receptors, Nerve Growth Factor

KW - Retroviridae

KW - T-Lymphocytes

KW - Thymidine Kinase

M3 - SCORING: Journal article

C2 - 9695974

VL - 102

SP - 566

EP - 574

JO - BRIT J HAEMATOL

JF - BRIT J HAEMATOL

SN - 0007-1048

IS - 2

ER -