Research ExplorerPublicationsHighly sensitive and specific novel biomarkers for the diagn...

Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder carcinoma

Standard

Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder carcinoma. / Kumar, Prashant; Nandi, Sayantani; Tan, Tuan Zea; Ler, Siok Ghee; Chia, Kee Seng; Lim, Wei-Yen; Bütow, Zentia; Vordos, Dimitrios; De la Taille, Alexandre; Al-Haddawi, Muthafar; Raida, Manfred; Beyer, Burkhard; Ricci, Estelle; Colombel, Marc; Chong, Tsung Wen; Chiong, Edmund; Soo, Ross; Park, Mi Kyoung; Ha, Hong Koo; Gunaratne, Jayantha; Thiery, Jean Paul.

In: ONCOTARGET, Vol. 6, No. 15, 30.05.2015, p. 13539-49.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kumar, P, Nandi, S, Tan, TZ, Ler, SG, Chia, KS, Lim, W-Y, Bütow, Z, Vordos, D, De la Taille, A, Al-Haddawi, M, Raida, M, Beyer, B, Ricci, E, Colombel, M, Chong, TW, Chiong, E, Soo, R, Park, MK, Ha, HK, Gunaratne, J & Thiery, JP 2015, 'Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder carcinoma', ONCOTARGET, vol. 6, no. 15, pp. 13539-49.

APA

Kumar, P., Nandi, S., Tan, T. Z., Ler, S. G., Chia, K. S., Lim, W-Y., Bütow, Z., Vordos, D., De la Taille, A., Al-Haddawi, M., Raida, M., Beyer, B., Ricci, E., Colombel, M., Chong, T. W., Chiong, E., Soo, R., Park, M. K., Ha, H. K., ... Thiery, J. P. (2015). Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder carcinoma. ONCOTARGET, 6(15), 13539-49.

Vancouver

Kumar P, Nandi S, Tan TZ, Ler SG, Chia KS, Lim W-Y et al. Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder carcinoma. ONCOTARGET. 2015 May 30;6(15):13539-49.

Bibtex

@article{369cda0536174a54ac8987d5bfc04c32,
title = "Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder carcinoma",
abstract = "Transitional bladder carcinoma (BCa) is prevalent in developed countries, particularly among men. Given that these tumors frequently recur or progress, the early detection and subsequent monitoring of BCa at different stages is critical. Current BCa diagnostic biomarkers are not sufficiently sensitive for substituting or complementing invasive cystoscopy. Here, we sought to identify a robust set of urine biomarkers for BCa detection. Using a high-resolution, mass spectrometry-based, quantitative proteomics approach, we measured, compared and validated protein variations in 451 voided urine samples from healthy subjects, non-bladder cancer patients and patients with non-invasive and invasive BCa. We identified five robust biomarkers: Coronin-1A, Apolipoprotein A4, Semenogelin-2, Gamma synuclein and DJ-1/PARK7. In diagnosing Ta/T1 BCa, these biomarkers achieved an AUC of 0.92 and 0.98, respectively, using ELISA and western blot data (sensitivity, 79.2% and 93.9%; specificity, 100% and 96.7%, respectively). In diagnosing T2/T3 BCa, an AUC of 0.94 and 1.0 was attained (sensitivity, 86.4% and 100%; specificity, 100%) using the same methods. Thus, our multiplex biomarker panel offers unprecedented accuracy for the diagnosis of BCa patients and provides the prospect for a non-invasive way to detect bladder cancer.",
author = "Prashant Kumar and Sayantani Nandi and Tan, {Tuan Zea} and Ler, {Siok Ghee} and Chia, {Kee Seng} and Wei-Yen Lim and Zentia B{\"u}tow and Dimitrios Vordos and {De la Taille}, Alexandre and Muthafar Al-Haddawi and Manfred Raida and Burkhard Beyer and Estelle Ricci and Marc Colombel and Chong, {Tsung Wen} and Edmund Chiong and Ross Soo and Park, {Mi Kyoung} and Ha, {Hong Koo} and Jayantha Gunaratne and Thiery, {Jean Paul}",
year = "2015",
month = may,
day = "30",
language = "English",
volume = "6",
pages = "13539--49",
journal = "ONCOTARGET",
issn = "1949-2553",
publisher = "IMPACT JOURNALS LLC",
number = "15",

}

RIS

TY - JOUR

T1 - Highly sensitive and specific novel biomarkers for the diagnosis of transitional bladder carcinoma

AU - Kumar, Prashant

AU - Nandi, Sayantani

AU - Tan, Tuan Zea

AU - Ler, Siok Ghee

AU - Chia, Kee Seng

AU - Lim, Wei-Yen

AU - Bütow, Zentia

AU - Vordos, Dimitrios

AU - De la Taille, Alexandre

AU - Al-Haddawi, Muthafar

AU - Raida, Manfred

AU - Beyer, Burkhard

AU - Ricci, Estelle

AU - Colombel, Marc

AU - Chong, Tsung Wen

AU - Chiong, Edmund

AU - Soo, Ross

AU - Park, Mi Kyoung

AU - Ha, Hong Koo

AU - Gunaratne, Jayantha

AU - Thiery, Jean Paul

PY - 2015/5/30

Y1 - 2015/5/30

N2 - Transitional bladder carcinoma (BCa) is prevalent in developed countries, particularly among men. Given that these tumors frequently recur or progress, the early detection and subsequent monitoring of BCa at different stages is critical. Current BCa diagnostic biomarkers are not sufficiently sensitive for substituting or complementing invasive cystoscopy. Here, we sought to identify a robust set of urine biomarkers for BCa detection. Using a high-resolution, mass spectrometry-based, quantitative proteomics approach, we measured, compared and validated protein variations in 451 voided urine samples from healthy subjects, non-bladder cancer patients and patients with non-invasive and invasive BCa. We identified five robust biomarkers: Coronin-1A, Apolipoprotein A4, Semenogelin-2, Gamma synuclein and DJ-1/PARK7. In diagnosing Ta/T1 BCa, these biomarkers achieved an AUC of 0.92 and 0.98, respectively, using ELISA and western blot data (sensitivity, 79.2% and 93.9%; specificity, 100% and 96.7%, respectively). In diagnosing T2/T3 BCa, an AUC of 0.94 and 1.0 was attained (sensitivity, 86.4% and 100%; specificity, 100%) using the same methods. Thus, our multiplex biomarker panel offers unprecedented accuracy for the diagnosis of BCa patients and provides the prospect for a non-invasive way to detect bladder cancer.

AB - Transitional bladder carcinoma (BCa) is prevalent in developed countries, particularly among men. Given that these tumors frequently recur or progress, the early detection and subsequent monitoring of BCa at different stages is critical. Current BCa diagnostic biomarkers are not sufficiently sensitive for substituting or complementing invasive cystoscopy. Here, we sought to identify a robust set of urine biomarkers for BCa detection. Using a high-resolution, mass spectrometry-based, quantitative proteomics approach, we measured, compared and validated protein variations in 451 voided urine samples from healthy subjects, non-bladder cancer patients and patients with non-invasive and invasive BCa. We identified five robust biomarkers: Coronin-1A, Apolipoprotein A4, Semenogelin-2, Gamma synuclein and DJ-1/PARK7. In diagnosing Ta/T1 BCa, these biomarkers achieved an AUC of 0.92 and 0.98, respectively, using ELISA and western blot data (sensitivity, 79.2% and 93.9%; specificity, 100% and 96.7%, respectively). In diagnosing T2/T3 BCa, an AUC of 0.94 and 1.0 was attained (sensitivity, 86.4% and 100%; specificity, 100%) using the same methods. Thus, our multiplex biomarker panel offers unprecedented accuracy for the diagnosis of BCa patients and provides the prospect for a non-invasive way to detect bladder cancer.

M3 - SCORING: Journal article

C2 - 25915536

VL - 6

SP - 13539

EP - 13549

JO - ONCOTARGET

JF - ONCOTARGET

SN - 1949-2553

IS - 15

ER -