High rate of premature chromosome condensation in human oocytes following microinjection with round-headed sperm: case report.
Standard
High rate of premature chromosome condensation in human oocytes following microinjection with round-headed sperm: case report. / Schmiady, Hardi; Schulze, Wolfgang; Scheiber, Ingrid; Pfüller, Bettina.
In: HUM REPROD, Vol. 20, No. 5, 5, 2005, p. 1319-1323.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - High rate of premature chromosome condensation in human oocytes following microinjection with round-headed sperm: case report.
AU - Schmiady, Hardi
AU - Schulze, Wolfgang
AU - Scheiber, Ingrid
AU - Pfüller, Bettina
PY - 2005
Y1 - 2005
N2 - A young couple proceeded to three ICSI treatment cycles because of male infertility. The semen samples varied between 10 x 10(6) and 36 x 10(6)/ml, 38 and 51% progressive motility but 0% normal morphology. Different types of sperm heads, mostly round-headed with varying spherical appearance (86%) were presented beside acephalic sperm (pinheads; 12%), both one- or two-tailed and the former also without a tail. Very few sperm (2%) exhibited slightly oval-shaped heads. Electron microscopy revealed the absence of the acrosome combined with disturbance of the chromatin condensation among the round-headed sperm. In all three cycles, the fertilization rate using the round-headed sperm fraction was very low with the best result of 2/18 (11%) two-pronucleate oocytes and one one-pronucleate oocyte obtained in the second ICSI cycle. The three oocytes cleaved and were transferred in the 3-4-cell stage without achieving a pregnancy. Of the 29 unfertilized and prepared oocytes from the last two cycles, 27 were informative and revealed the maternal metaphase II chromosomes in the haploid range and a high rate (85%) of premature chromosome condensation (PCC) of the sperm nucleus with remarkable variation in the degree of condensation. Thus, it appears that nearly all round-headed sperm from this patient were incapable of oocyte activation after ICSI, which could be due to non-release (or absence) of an activating factor. As a consequence, PCC was induced in the sperm nuclei by the chromosome condensing factors which were still active in the oopasm of the arrested oocytes.
AB - A young couple proceeded to three ICSI treatment cycles because of male infertility. The semen samples varied between 10 x 10(6) and 36 x 10(6)/ml, 38 and 51% progressive motility but 0% normal morphology. Different types of sperm heads, mostly round-headed with varying spherical appearance (86%) were presented beside acephalic sperm (pinheads; 12%), both one- or two-tailed and the former also without a tail. Very few sperm (2%) exhibited slightly oval-shaped heads. Electron microscopy revealed the absence of the acrosome combined with disturbance of the chromatin condensation among the round-headed sperm. In all three cycles, the fertilization rate using the round-headed sperm fraction was very low with the best result of 2/18 (11%) two-pronucleate oocytes and one one-pronucleate oocyte obtained in the second ICSI cycle. The three oocytes cleaved and were transferred in the 3-4-cell stage without achieving a pregnancy. Of the 29 unfertilized and prepared oocytes from the last two cycles, 27 were informative and revealed the maternal metaphase II chromosomes in the haploid range and a high rate (85%) of premature chromosome condensation (PCC) of the sperm nucleus with remarkable variation in the degree of condensation. Thus, it appears that nearly all round-headed sperm from this patient were incapable of oocyte activation after ICSI, which could be due to non-release (or absence) of an activating factor. As a consequence, PCC was induced in the sperm nuclei by the chromosome condensing factors which were still active in the oopasm of the arrested oocytes.
M3 - SCORING: Zeitschriftenaufsatz
VL - 20
SP - 1319
EP - 1323
JO - HUM REPROD
JF - HUM REPROD
SN - 0268-1161
IS - 5
M1 - 5
ER -