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High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments

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High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments. / Hagemann, Carsten; Meyer, Christoph; Stojic, Jelena; Eicker, Sven; Gerngras, Stefanie; Kühnel, Siglinde; Roosen, Klaus; Vince, Giles Hamilton.

In: J NEUROSCI METH, Vol. 156, No. 1-2, 30.09.2006, p. 194-202.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hagemann, C, Meyer, C, Stojic, J, Eicker, S, Gerngras, S, Kühnel, S, Roosen, K & Vince, GH 2006, 'High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments', J NEUROSCI METH, vol. 156, no. 1-2, pp. 194-202. https://doi.org/10.1016/j.jneumeth.2006.03.003

APA

Hagemann, C., Meyer, C., Stojic, J., Eicker, S., Gerngras, S., Kühnel, S., Roosen, K., & Vince, G. H. (2006). High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments. J NEUROSCI METH, 156(1-2), 194-202. https://doi.org/10.1016/j.jneumeth.2006.03.003

Vancouver

Hagemann C, Meyer C, Stojic J, Eicker S, Gerngras S, Kühnel S et al. High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments. J NEUROSCI METH. 2006 Sep 30;156(1-2):194-202. https://doi.org/10.1016/j.jneumeth.2006.03.003

Bibtex

@article{34c8f205781a45d78a51690c1012a286,
title = "High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments",
abstract = "In order to investigate the impact of signalling proteins on the phenotype and malignant behavior of glioblastoma cells, we optimized the transfection procedure of human glioblastoma cell lines U251, U373, GaMG and of primary cells obtained from a patient's tumor using nucleofection technology in conjunction with plasmid pmaxGFP. We describe the optimization procedure, show that a high percentage of the cells can be transfected and that nucleofection does not cause phenotypic alterations of the cells. Therefore, we conclude that nucleofection is a highly efficient tool to deliver plasmids for transient protein overexpression and siRNA for specific protein knock-down to different glioblastoma cell lines or primary cells.",
keywords = "Blotting, Western, Brain Neoplasms, Cell Line, Tumor, Cell Movement, Cell Nucleus, Cell Proliferation, Cytological Techniques, Electroporation, Glial Fibrillary Acidic Protein, Glioma, Green Fluorescent Proteins, Humans, Immunohistochemistry, Neurons, Phenotype, Plasmids, RNA, Small Interfering, Reverse Transcriptase Polymerase Chain Reaction, Transfection",
author = "Carsten Hagemann and Christoph Meyer and Jelena Stojic and Sven Eicker and Stefanie Gerngras and Siglinde K{\"u}hnel and Klaus Roosen and Vince, {Giles Hamilton}",
year = "2006",
month = sep,
day = "30",
doi = "10.1016/j.jneumeth.2006.03.003",
language = "English",
volume = "156",
pages = "194--202",
journal = "J NEUROSCI METH",
issn = "0165-0270",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments

AU - Hagemann, Carsten

AU - Meyer, Christoph

AU - Stojic, Jelena

AU - Eicker, Sven

AU - Gerngras, Stefanie

AU - Kühnel, Siglinde

AU - Roosen, Klaus

AU - Vince, Giles Hamilton

PY - 2006/9/30

Y1 - 2006/9/30

N2 - In order to investigate the impact of signalling proteins on the phenotype and malignant behavior of glioblastoma cells, we optimized the transfection procedure of human glioblastoma cell lines U251, U373, GaMG and of primary cells obtained from a patient's tumor using nucleofection technology in conjunction with plasmid pmaxGFP. We describe the optimization procedure, show that a high percentage of the cells can be transfected and that nucleofection does not cause phenotypic alterations of the cells. Therefore, we conclude that nucleofection is a highly efficient tool to deliver plasmids for transient protein overexpression and siRNA for specific protein knock-down to different glioblastoma cell lines or primary cells.

AB - In order to investigate the impact of signalling proteins on the phenotype and malignant behavior of glioblastoma cells, we optimized the transfection procedure of human glioblastoma cell lines U251, U373, GaMG and of primary cells obtained from a patient's tumor using nucleofection technology in conjunction with plasmid pmaxGFP. We describe the optimization procedure, show that a high percentage of the cells can be transfected and that nucleofection does not cause phenotypic alterations of the cells. Therefore, we conclude that nucleofection is a highly efficient tool to deliver plasmids for transient protein overexpression and siRNA for specific protein knock-down to different glioblastoma cell lines or primary cells.

KW - Blotting, Western

KW - Brain Neoplasms

KW - Cell Line, Tumor

KW - Cell Movement

KW - Cell Nucleus

KW - Cell Proliferation

KW - Cytological Techniques

KW - Electroporation

KW - Glial Fibrillary Acidic Protein

KW - Glioma

KW - Green Fluorescent Proteins

KW - Humans

KW - Immunohistochemistry

KW - Neurons

KW - Phenotype

KW - Plasmids

KW - RNA, Small Interfering

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Transfection

U2 - 10.1016/j.jneumeth.2006.03.003

DO - 10.1016/j.jneumeth.2006.03.003

M3 - SCORING: Journal article

C2 - 16621008

VL - 156

SP - 194

EP - 202

JO - J NEUROSCI METH

JF - J NEUROSCI METH

SN - 0165-0270

IS - 1-2

ER -