High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells.
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High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells. / Lee, Min Young; Esra, Cagavi Bozkulak; Schliffke, Simon; Amos, Peter J; Ren, Yongming; Ge, Xin; Ehrlich, Barbara E; Qyang, Yibing.
In: BIOCHEM BIOPH RES CO, Vol. 416, No. 1-2, 1-2, 2011, p. 51-57.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - High density cultures of embryoid bodies enhanced cardiac differentiation of murine embryonic stem cells.
AU - Lee, Min Young
AU - Esra, Cagavi Bozkulak
AU - Schliffke, Simon
AU - Amos, Peter J
AU - Ren, Yongming
AU - Ge, Xin
AU - Ehrlich, Barbara E
AU - Qyang, Yibing
PY - 2011
Y1 - 2011
N2 - Murine embryonic stem cell (mESC)-derived cardiomyocytes represent a promising source of cells for use in the development of models for studying early cardiac development as well as cell-based therapies in postnatal pathologies. Here, we report a highly efficient cardiac differentiation system in which high density embryoid body (EB) cultures leads to a marked increase of cardiomyocytes production from multiple mESC lines without the addition of any cardiogenic growth factors. Our results show that high density EB cultures significantly increase the yield of functional cardiomyocytes, which express typical cardiac markers, exhibit normal rhythmic Ca(2+) transients, and respond to both ?-adrenergic and electric stimulations. During the differentiation period, the inhibition of bone morphogenetic protein (BMP) signaling significantly attenuates the increase of cardiac differentiation as well as the increased expression of cardiac-specific genes, NK2 transcription factor related 5 (Nkx2.5) and myosin light chain 2v (Mlc2v) by high density EB cultures. Therefore, we believe that we offer a novel and efficient means of cardiomyocyte production for practical use of mESCs in cardiac regenerative medicine.
AB - Murine embryonic stem cell (mESC)-derived cardiomyocytes represent a promising source of cells for use in the development of models for studying early cardiac development as well as cell-based therapies in postnatal pathologies. Here, we report a highly efficient cardiac differentiation system in which high density embryoid body (EB) cultures leads to a marked increase of cardiomyocytes production from multiple mESC lines without the addition of any cardiogenic growth factors. Our results show that high density EB cultures significantly increase the yield of functional cardiomyocytes, which express typical cardiac markers, exhibit normal rhythmic Ca(2+) transients, and respond to both ?-adrenergic and electric stimulations. During the differentiation period, the inhibition of bone morphogenetic protein (BMP) signaling significantly attenuates the increase of cardiac differentiation as well as the increased expression of cardiac-specific genes, NK2 transcription factor related 5 (Nkx2.5) and myosin light chain 2v (Mlc2v) by high density EB cultures. Therefore, we believe that we offer a novel and efficient means of cardiomyocyte production for practical use of mESCs in cardiac regenerative medicine.
KW - Animals
KW - Mice
KW - Mice, Inbred C57BL
KW - Cell Count
KW - Calcium/metabolism
KW - Bone Morphogenetic Proteins/antagonists & inhibitors
KW - Cell Culture Techniques
KW - Cell Differentiation
KW - Embryoid Bodies/cytology
KW - Embryonic Stem Cells/cytology
KW - Myocytes, Cardiac/cytology
KW - Animals
KW - Mice
KW - Mice, Inbred C57BL
KW - Cell Count
KW - Calcium/metabolism
KW - Bone Morphogenetic Proteins/antagonists & inhibitors
KW - Cell Culture Techniques
KW - Cell Differentiation
KW - Embryoid Bodies/cytology
KW - Embryonic Stem Cells/cytology
KW - Myocytes, Cardiac/cytology
M3 - SCORING: Journal article
VL - 416
SP - 51
EP - 57
JO - BIOCHEM BIOPH RES CO
JF - BIOCHEM BIOPH RES CO
SN - 0006-291X
IS - 1-2
M1 - 1-2
ER -
