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Herpes virus saimiri-transformed human T lymphocytes

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Herpes virus saimiri-transformed human T lymphocytes : normal functional phenotype and preserved T cell receptor signalling. / Mittrücker, H W; Müller-Fleckenstein, I; Fleckenstein, B; Fleischer, B.

In: INT IMMUNOL, Vol. 5, No. 8, 01.08.1993, p. 985-90.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Mittrücker, HW, Müller-Fleckenstein, I, Fleckenstein, B & Fleischer, B 1993, 'Herpes virus saimiri-transformed human T lymphocytes: normal functional phenotype and preserved T cell receptor signalling', INT IMMUNOL, vol. 5, no. 8, pp. 985-90.

APA

Mittrücker, H. W., Müller-Fleckenstein, I., Fleckenstein, B., & Fleischer, B. (1993). Herpes virus saimiri-transformed human T lymphocytes: normal functional phenotype and preserved T cell receptor signalling. INT IMMUNOL, 5(8), 985-90.

Vancouver

Mittrücker HW, Müller-Fleckenstein I, Fleckenstein B, Fleischer B. Herpes virus saimiri-transformed human T lymphocytes: normal functional phenotype and preserved T cell receptor signalling. INT IMMUNOL. 1993 Aug 1;5(8):985-90.

Bibtex

@article{69c4370398d94182a74b5961332bda72,
title = "Herpes virus saimiri-transformed human T lymphocytes: normal functional phenotype and preserved T cell receptor signalling",
abstract = "Herpes virus saimiri (HVS), a primate herpes virus, transforms human CD4+ and CD8+ T lymphocytes to continuous growth in vitro. We have previously shown that HVS-transformed human T cells (HVS-T cells) respond to stimulation via CD2 with autocrine growth. In the present study we have investigated the functional characteristics of HVS-T cells. We describe that these cells can perform all the functions of normal T cells, i.e. cytokine secretion, cytotoxicity, and exocytosis of granule esterases. All these activities can be triggered via CD2 by binding to its natural ligand or via the TCR, e.g. by anti-TCR antibodies, by recognition of a bacterial superantigen and by MHC-restricted recognition of specific antigen. The pattern of tyrosine-phosphorylated proteins after TCR triggering was identical in HVS-T cells and normal T cells. We conclude that HVS-T cells can respond to TCR-mediated signals with the functions of normal T lymphocytes. Furthermore, HVS-T cells are the only transformed human T cells that can be specifically triggered by cytotoxicity and esterase release. The finding that the TCR functions normally in these cells will make HVS a convenient means to immortalize antigen-specific human T lymphocytes.",
keywords = "Antigens, Antigens, CD2, Antigens, Differentiation, T-Lymphocyte, Cell Line, Transformed, Cell Transformation, Viral, Herpesvirus 2, Saimiriine, Humans, Interleukin-2, Lymphocyte Activation, Phenotype, Receptors, Antigen, T-Cell, Receptors, Immunologic, T-Lymphocytes",
author = "Mittr{\"u}cker, {H W} and I M{\"u}ller-Fleckenstein and B Fleckenstein and B Fleischer",
year = "1993",
month = aug,
day = "1",
language = "English",
volume = "5",
pages = "985--90",
journal = "INT IMMUNOL",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "8",

}

RIS

TY - JOUR

T1 - Herpes virus saimiri-transformed human T lymphocytes

T2 - normal functional phenotype and preserved T cell receptor signalling

AU - Mittrücker, H W

AU - Müller-Fleckenstein, I

AU - Fleckenstein, B

AU - Fleischer, B

PY - 1993/8/1

Y1 - 1993/8/1

N2 - Herpes virus saimiri (HVS), a primate herpes virus, transforms human CD4+ and CD8+ T lymphocytes to continuous growth in vitro. We have previously shown that HVS-transformed human T cells (HVS-T cells) respond to stimulation via CD2 with autocrine growth. In the present study we have investigated the functional characteristics of HVS-T cells. We describe that these cells can perform all the functions of normal T cells, i.e. cytokine secretion, cytotoxicity, and exocytosis of granule esterases. All these activities can be triggered via CD2 by binding to its natural ligand or via the TCR, e.g. by anti-TCR antibodies, by recognition of a bacterial superantigen and by MHC-restricted recognition of specific antigen. The pattern of tyrosine-phosphorylated proteins after TCR triggering was identical in HVS-T cells and normal T cells. We conclude that HVS-T cells can respond to TCR-mediated signals with the functions of normal T lymphocytes. Furthermore, HVS-T cells are the only transformed human T cells that can be specifically triggered by cytotoxicity and esterase release. The finding that the TCR functions normally in these cells will make HVS a convenient means to immortalize antigen-specific human T lymphocytes.

AB - Herpes virus saimiri (HVS), a primate herpes virus, transforms human CD4+ and CD8+ T lymphocytes to continuous growth in vitro. We have previously shown that HVS-transformed human T cells (HVS-T cells) respond to stimulation via CD2 with autocrine growth. In the present study we have investigated the functional characteristics of HVS-T cells. We describe that these cells can perform all the functions of normal T cells, i.e. cytokine secretion, cytotoxicity, and exocytosis of granule esterases. All these activities can be triggered via CD2 by binding to its natural ligand or via the TCR, e.g. by anti-TCR antibodies, by recognition of a bacterial superantigen and by MHC-restricted recognition of specific antigen. The pattern of tyrosine-phosphorylated proteins after TCR triggering was identical in HVS-T cells and normal T cells. We conclude that HVS-T cells can respond to TCR-mediated signals with the functions of normal T lymphocytes. Furthermore, HVS-T cells are the only transformed human T cells that can be specifically triggered by cytotoxicity and esterase release. The finding that the TCR functions normally in these cells will make HVS a convenient means to immortalize antigen-specific human T lymphocytes.

KW - Antigens

KW - Antigens, CD2

KW - Antigens, Differentiation, T-Lymphocyte

KW - Cell Line, Transformed

KW - Cell Transformation, Viral

KW - Herpesvirus 2, Saimiriine

KW - Humans

KW - Interleukin-2

KW - Lymphocyte Activation

KW - Phenotype

KW - Receptors, Antigen, T-Cell

KW - Receptors, Immunologic

KW - T-Lymphocytes

M3 - SCORING: Journal article

C2 - 8104475

VL - 5

SP - 985

EP - 990

JO - INT IMMUNOL

JF - INT IMMUNOL

SN - 0953-8178

IS - 8

ER -