Hepatic tissue engineering on 3-dimensional biodegradable polymers within a pulsatile flow bioreactor.
Standard
Hepatic tissue engineering on 3-dimensional biodegradable polymers within a pulsatile flow bioreactor. / Török, E; Pollok, Jörg-Matthias; Ma, P X; Vogel, C; Dandri-Petersen, Maura; Petersen, J; Burda, M R; Kaufmann, P M; Kluth, D; Rogiers, X.
In: DIGEST SURG, Vol. 18, No. 3, 3, 2001, p. 196-203.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Hepatic tissue engineering on 3-dimensional biodegradable polymers within a pulsatile flow bioreactor.
AU - Török, E
AU - Pollok, Jörg-Matthias
AU - Ma, P X
AU - Vogel, C
AU - Dandri-Petersen, Maura
AU - Petersen, J
AU - Burda, M R
AU - Kaufmann, P M
AU - Kluth, D
AU - Rogiers, X
PY - 2001
Y1 - 2001
N2 - BACKGROUND: An optimal method for hepatocyte transplantation is not yet determined. With the principles of tissue engineering in vitro conditioning of hepatocytes on biodegradable polymer in a flow bioreactor before implantation forming spheroids may achieve increased cell mass and function to replace lost organ function in vivo. METHODS: Biodegradable poly-L-lactic (PLLA) polymer discs were seeded with rat hepatocytes in a concentration of 10 x 10(6) cells per ml and exposed to a medium flow of 24 ml/min for 1, 2, 4 and 6 days. The number and diameter of spheroidal aggregates was measured by phase-contrast microscopy. H;E histology was performed. Albumin production as hepatocyte specific function was determined by ELISA. RESULTS: Spheroids of viable hepatocytes of 50-200 microm in diameter were formed. Both the number and diameter of the spheroids increased during the first 2 days and then remained constant until day 6. Albumin production was maintained throughout the culture period. CONCLUSION: Short (2- 3 days) pre-transplant conditioning of hepatocytes in a flow bioreactor on biodegradable PLLA resulted in formation of spheroids with a liver-like morphology and preserved specific metabolic function. Tissue engineered hepatocyte spheroids on polymer may represent a functionally active and easy transplantable neotissue and may serve as an in vivo substitute for lost liver function.
AB - BACKGROUND: An optimal method for hepatocyte transplantation is not yet determined. With the principles of tissue engineering in vitro conditioning of hepatocytes on biodegradable polymer in a flow bioreactor before implantation forming spheroids may achieve increased cell mass and function to replace lost organ function in vivo. METHODS: Biodegradable poly-L-lactic (PLLA) polymer discs were seeded with rat hepatocytes in a concentration of 10 x 10(6) cells per ml and exposed to a medium flow of 24 ml/min for 1, 2, 4 and 6 days. The number and diameter of spheroidal aggregates was measured by phase-contrast microscopy. H;E histology was performed. Albumin production as hepatocyte specific function was determined by ELISA. RESULTS: Spheroids of viable hepatocytes of 50-200 microm in diameter were formed. Both the number and diameter of the spheroids increased during the first 2 days and then remained constant until day 6. Albumin production was maintained throughout the culture period. CONCLUSION: Short (2- 3 days) pre-transplant conditioning of hepatocytes in a flow bioreactor on biodegradable PLLA resulted in formation of spheroids with a liver-like morphology and preserved specific metabolic function. Tissue engineered hepatocyte spheroids on polymer may represent a functionally active and easy transplantable neotissue and may serve as an in vivo substitute for lost liver function.
M3 - SCORING: Zeitschriftenaufsatz
VL - 18
SP - 196
EP - 203
JO - DIGEST SURG
JF - DIGEST SURG
SN - 0253-4886
IS - 3
M1 - 3
ER -
