Hepatic stellate cell lipid droplets: a specialized lipid droplet for retinoid storage.
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Hepatic stellate cell lipid droplets: a specialized lipid droplet for retinoid storage. / Blaner, William S; O'Byrne, Sheila M; Wongsiriroj, Nuttaporn; Kluwe, Johannes; D'Ambrosio, Diana M; Jiang, Hongfeng; Schwabe, Robert F; Hillman, Elizabeth M C; Piantedosi, Roseann; Libien, Jenny.
In: BBA-MOL CELL BIOL L, Vol. 1791, No. 6, 6, 2009, p. 467-473.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Hepatic stellate cell lipid droplets: a specialized lipid droplet for retinoid storage.
AU - Blaner, William S
AU - O'Byrne, Sheila M
AU - Wongsiriroj, Nuttaporn
AU - Kluwe, Johannes
AU - D'Ambrosio, Diana M
AU - Jiang, Hongfeng
AU - Schwabe, Robert F
AU - Hillman, Elizabeth M C
AU - Piantedosi, Roseann
AU - Libien, Jenny
PY - 2009
Y1 - 2009
N2 - The majority of retinoid (vitamin A and its metabolites) present in the body of a healthy vertebrate is contained within lipid droplets present in the cytoplasm of hepatic stellate cells (HSCs). Two types of lipid droplets have been identified through histological analysis of HSCs within the liver: smaller droplets bounded by a unit membrane and larger membrane-free droplets. Dietary retinoid intake but not triglyceride intake markedly influences the number and size of HSC lipid droplets. The lipids present in rat HSC lipid droplets include retinyl ester, triglyceride, cholesteryl ester, cholesterol, phospholipids and free fatty acids. Retinyl ester and triglyceride are present at similar concentrations, and together these two classes of lipid account for approximately three-quarters of the total lipid in HSC lipid droplets. Both adipocyte-differentiation related protein and TIP47 have been identified by immunohistochemical analysis to be present in HSC lipid droplets. Lecithin:retinol acyltransferase (LRAT), an enzyme responsible for all retinyl ester synthesis within the liver, is required for HSC lipid droplet formation, since Lrat-deficient mice completely lack HSC lipid droplets. When HSCs become activated in response to hepatic injury, the lipid droplets and their retinoid contents are rapidly lost. Although loss of HSC lipid droplets is a hallmark of developing liver disease, it is not known whether this contributes to disease development or occurs simply as a consequence of disease progression. Collectively, the available information suggests that HSC lipid droplets are specialized organelles for hepatic retinoid storage and that loss of HSC lipid droplets may contribute to the development of hepatic disease.
AB - The majority of retinoid (vitamin A and its metabolites) present in the body of a healthy vertebrate is contained within lipid droplets present in the cytoplasm of hepatic stellate cells (HSCs). Two types of lipid droplets have been identified through histological analysis of HSCs within the liver: smaller droplets bounded by a unit membrane and larger membrane-free droplets. Dietary retinoid intake but not triglyceride intake markedly influences the number and size of HSC lipid droplets. The lipids present in rat HSC lipid droplets include retinyl ester, triglyceride, cholesteryl ester, cholesterol, phospholipids and free fatty acids. Retinyl ester and triglyceride are present at similar concentrations, and together these two classes of lipid account for approximately three-quarters of the total lipid in HSC lipid droplets. Both adipocyte-differentiation related protein and TIP47 have been identified by immunohistochemical analysis to be present in HSC lipid droplets. Lecithin:retinol acyltransferase (LRAT), an enzyme responsible for all retinyl ester synthesis within the liver, is required for HSC lipid droplet formation, since Lrat-deficient mice completely lack HSC lipid droplets. When HSCs become activated in response to hepatic injury, the lipid droplets and their retinoid contents are rapidly lost. Although loss of HSC lipid droplets is a hallmark of developing liver disease, it is not known whether this contributes to disease development or occurs simply as a consequence of disease progression. Collectively, the available information suggests that HSC lipid droplets are specialized organelles for hepatic retinoid storage and that loss of HSC lipid droplets may contribute to the development of hepatic disease.
KW - Animals
KW - Humans
KW - Acyltransferases metabolism
KW - Hepatic Stellate Cells metabolism
KW - Lipid Metabolism
KW - Liver Diseases metabolism
KW - Organelle Size
KW - Organelles metabolism
KW - Retinoids metabolism
KW - Triglycerides metabolism
KW - Animals
KW - Humans
KW - Acyltransferases metabolism
KW - Hepatic Stellate Cells metabolism
KW - Lipid Metabolism
KW - Liver Diseases metabolism
KW - Organelle Size
KW - Organelles metabolism
KW - Retinoids metabolism
KW - Triglycerides metabolism
M3 - SCORING: Zeitschriftenaufsatz
VL - 1791
SP - 467
EP - 473
JO - BBA-MOL CELL BIOL L
JF - BBA-MOL CELL BIOL L
SN - 1388-1981
IS - 6
M1 - 6
ER -
