Heme oxygenase-1 suppresses a pro-inflammatory phenotype in monocytes and determines endothelial function and arterial hypertension in mice and humans
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Heme oxygenase-1 suppresses a pro-inflammatory phenotype in monocytes and determines endothelial function and arterial hypertension in mice and humans. / Wenzel, Philip; Rossmann, Heidi; Müller, Christian; Kossmann, Sabine; Oelze, Matthias; Schulz, Andreas; Arnold, Natalie; Simsek, Canan; Lagrange, Jeremy; Klemz, Roman; Schönfelder, Tanja; Brandt, Moritz; Karbach, Susanne H; Knorr, Maike; Finger, Stefanie; Neukirch, Carolin; Häuser, Friederike; Beutel, Manfred E; Kröller-Schön, Swenja; Schulz, Eberhard; Schnabel, Renate B; Lackner, Karl; Wild, Philipp S; Zeller, Tanja; Daiber, Andreas; Blankenberg, Stefan; Münzel, Thomas.
In: EUR HEART J, Vol. 36, No. 48, 21.12.2015, p. 3437-46.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Heme oxygenase-1 suppresses a pro-inflammatory phenotype in monocytes and determines endothelial function and arterial hypertension in mice and humans
AU - Wenzel, Philip
AU - Rossmann, Heidi
AU - Müller, Christian
AU - Kossmann, Sabine
AU - Oelze, Matthias
AU - Schulz, Andreas
AU - Arnold, Natalie
AU - Simsek, Canan
AU - Lagrange, Jeremy
AU - Klemz, Roman
AU - Schönfelder, Tanja
AU - Brandt, Moritz
AU - Karbach, Susanne H
AU - Knorr, Maike
AU - Finger, Stefanie
AU - Neukirch, Carolin
AU - Häuser, Friederike
AU - Beutel, Manfred E
AU - Kröller-Schön, Swenja
AU - Schulz, Eberhard
AU - Schnabel, Renate B
AU - Lackner, Karl
AU - Wild, Philipp S
AU - Zeller, Tanja
AU - Daiber, Andreas
AU - Blankenberg, Stefan
AU - Münzel, Thomas
N1 - Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2015. For permissions please email: journals.permissions@oup.com.
PY - 2015/12/21
Y1 - 2015/12/21
N2 - AIMS: Heme oxygenase-1 (HO-1) confers protection to the vasculature and suppresses inflammatory properties of monocytes and macrophages. It is unclear how HO-1 determines the extent of vascular dysfunction in mice and humans.METHODS AND RESULTS: Decreased HO-1 activity and expression was paralleled by increased aortic expression and activity of the nicotinamide dinucleotide phosphate oxidase Nox2 in HO-1 deficient Hmox1⁻/⁻ and Hmox1(⁺/⁻) compared with Hmox1⁺/⁺ mice. When subjected to angiotensin II-infusion, streptozotocin-induced diabetes mellitus and aging, HO-1 deficient mice showed increased vascular dysfunction inversely correlated with HO activity. In a primary prevention population-based cohort, we assessed length polymorphisms of the HMOX1 promoter region and established a bipolar frequency pattern of allele length (long vs. short repeats) in 4937 individuals. Monocytic HMOX1 mRNA expression was positively correlated with flow-mediated dilation and inversely with CD14 mRNA expression indicating pro-inflammatory monocytes in 733 hypertensive individuals of this cohort. Hmox1⁻/⁻ mice showed drastically increased expression of the chemokine receptor CCR2 in monocytes and the aorta. Angiotensin II-infused Hmox1⁻/⁻ mice had amplified endothelial inflammation in vivo, significantly increased aortic infiltration of pro-inflammatory CD11b⁺ Ly6C(hi) monocytes and Ly6G⁺ neutrophils and were marked by Ly6C(hi) monocytosis in the circulation and an increased blood pressure response. Finally, individuals with unfavourable HMOX1 gene promoter length had increased prevalence of arterial hypertension and reduced cumulative survival after a median follow-up of 7.23 years.CONCLUSIONS: Heme oxygenase-1 is a regulator of vascular function in hypertension via determining the phenotype of inflammatory circulating and infiltrating monocytes with possible implications for all-cause mortality.
AB - AIMS: Heme oxygenase-1 (HO-1) confers protection to the vasculature and suppresses inflammatory properties of monocytes and macrophages. It is unclear how HO-1 determines the extent of vascular dysfunction in mice and humans.METHODS AND RESULTS: Decreased HO-1 activity and expression was paralleled by increased aortic expression and activity of the nicotinamide dinucleotide phosphate oxidase Nox2 in HO-1 deficient Hmox1⁻/⁻ and Hmox1(⁺/⁻) compared with Hmox1⁺/⁺ mice. When subjected to angiotensin II-infusion, streptozotocin-induced diabetes mellitus and aging, HO-1 deficient mice showed increased vascular dysfunction inversely correlated with HO activity. In a primary prevention population-based cohort, we assessed length polymorphisms of the HMOX1 promoter region and established a bipolar frequency pattern of allele length (long vs. short repeats) in 4937 individuals. Monocytic HMOX1 mRNA expression was positively correlated with flow-mediated dilation and inversely with CD14 mRNA expression indicating pro-inflammatory monocytes in 733 hypertensive individuals of this cohort. Hmox1⁻/⁻ mice showed drastically increased expression of the chemokine receptor CCR2 in monocytes and the aorta. Angiotensin II-infused Hmox1⁻/⁻ mice had amplified endothelial inflammation in vivo, significantly increased aortic infiltration of pro-inflammatory CD11b⁺ Ly6C(hi) monocytes and Ly6G⁺ neutrophils and were marked by Ly6C(hi) monocytosis in the circulation and an increased blood pressure response. Finally, individuals with unfavourable HMOX1 gene promoter length had increased prevalence of arterial hypertension and reduced cumulative survival after a median follow-up of 7.23 years.CONCLUSIONS: Heme oxygenase-1 is a regulator of vascular function in hypertension via determining the phenotype of inflammatory circulating and infiltrating monocytes with possible implications for all-cause mortality.
KW - Animals
KW - Cross-Sectional Studies
KW - Endothelium, Vascular/physiopathology
KW - Female
KW - Heme Oxygenase-1/deficiency
KW - Humans
KW - Hypertension/mortality
KW - Male
KW - Mice
KW - Monocytes/physiology
KW - Neutrophils/physiology
KW - Oxidative Stress/physiology
KW - Phenotype
KW - Polymorphism, Genetic
KW - RNA, Messenger/metabolism
U2 - 10.1093/eurheartj/ehv544
DO - 10.1093/eurheartj/ehv544
M3 - SCORING: Journal article
C2 - 26516175
VL - 36
SP - 3437
EP - 3446
JO - EUR HEART J
JF - EUR HEART J
SN - 0195-668X
IS - 48
ER -