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Hematopoietic stem cells develop in the absence of endothelial cadherin 5 expression

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Hematopoietic stem cells develop in the absence of endothelial cadherin 5 expression. / Anderson, Heidi; Patch, Taylor C; Reddy, Pavankumar N G; Hagedorn, Elliott J; Kim, Peter G; Soltis, Kathleen A; Chen, Michael J; Tamplin, Owen J; Frye, Maike; MacLean, Glenn A; Hübner, Kathleen; Bauer, Daniel E; Kanki, John P; Vogin, Guillaume; Huston, Nicholas C; Nguyen, Minh; Fujiwara, Yuko; Paw, Barry H; Vestweber, Dietmar; Zon, Leonard I; Orkin, Stuart H; Daley, George Q; Shah, Dhvanit I.

In: BLOOD, Vol. 126, No. 26, 24.12.2015, p. 2811-20.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Anderson, H, Patch, TC, Reddy, PNG, Hagedorn, EJ, Kim, PG, Soltis, KA, Chen, MJ, Tamplin, OJ, Frye, M, MacLean, GA, Hübner, K, Bauer, DE, Kanki, JP, Vogin, G, Huston, NC, Nguyen, M, Fujiwara, Y, Paw, BH, Vestweber, D, Zon, LI, Orkin, SH, Daley, GQ & Shah, DI 2015, 'Hematopoietic stem cells develop in the absence of endothelial cadherin 5 expression', BLOOD, vol. 126, no. 26, pp. 2811-20. https://doi.org/10.1182/blood-2015-07-659276

APA

Anderson, H., Patch, T. C., Reddy, P. N. G., Hagedorn, E. J., Kim, P. G., Soltis, K. A., Chen, M. J., Tamplin, O. J., Frye, M., MacLean, G. A., Hübner, K., Bauer, D. E., Kanki, J. P., Vogin, G., Huston, N. C., Nguyen, M., Fujiwara, Y., Paw, B. H., Vestweber, D., ... Shah, D. I. (2015). Hematopoietic stem cells develop in the absence of endothelial cadherin 5 expression. BLOOD, 126(26), 2811-20. https://doi.org/10.1182/blood-2015-07-659276

Vancouver

Anderson H, Patch TC, Reddy PNG, Hagedorn EJ, Kim PG, Soltis KA et al. Hematopoietic stem cells develop in the absence of endothelial cadherin 5 expression. BLOOD. 2015 Dec 24;126(26):2811-20. https://doi.org/10.1182/blood-2015-07-659276

Bibtex

@article{b6da4a4918c6430abac0a6ba4bc03aad,
title = "Hematopoietic stem cells develop in the absence of endothelial cadherin 5 expression",
abstract = "Rare endothelial cells in the aorta-gonad-mesonephros (AGM) transition into hematopoietic stem cells (HSCs) during embryonic development. Lineage tracing experiments indicate that HSCs emerge from cadherin 5 (Cdh5; vascular endothelial-cadherin)(+) endothelial precursors, and isolated populations of Cdh5(+) cells from mouse embryos and embryonic stem cells can be differentiated into hematopoietic cells. Cdh5 has also been widely implicated as a marker of AGM-derived hemogenic endothelial cells. Because Cdh5(-/-) mice embryos die before the first HSCs emerge, it is unknown whether Cdh5 has a direct role in HSC emergence. Our previous genetic screen yielded malbec (mlb(bw306)), a zebrafish mutant for cdh5, with normal embryonic and definitive blood. Using time-lapse confocal imaging, parabiotic surgical pairing of zebrafish embryos, and blastula transplantation assays, we show that HSCs emerge, migrate, engraft, and differentiate in the absence of cdh5 expression. By tracing Cdh5(-/-)green fluorescent protein (GFP)(+/+) cells in chimeric mice, we demonstrated that Cdh5(-/-)GFP(+/+) HSCs emerging from embryonic day 10.5 and 11.5 (E10.5 and E11.5) AGM or derived from E13.5 fetal liver not only differentiate into hematopoietic colonies but also engraft and reconstitute multilineage adult blood. We also developed a conditional mouse Cdh5 knockout (Cdh5(flox/flox):Scl-Cre-ER(T)) and demonstrated that multipotent hematopoietic colonies form despite the absence of Cdh5. These data establish that Cdh5, a marker of hemogenic endothelium in the AGM, is dispensable for the transition of hemogenic endothelium to HSCs.",
keywords = "Animals, Antigens, CD, Cadherins, Cell Differentiation, Cell Lineage, Electroporation, Embryo, Mammalian, Embryo, Nonmammalian, Flow Cytometry, Hemangioblasts, Hematopoiesis, Hematopoietic Stem Cells, Immunohistochemistry, Mesonephros, Mice, Mice, Knockout, Microscopy, Confocal, Zebrafish, Journal Article",
author = "Heidi Anderson and Patch, {Taylor C} and Reddy, {Pavankumar N G} and Hagedorn, {Elliott J} and Kim, {Peter G} and Soltis, {Kathleen A} and Chen, {Michael J} and Tamplin, {Owen J} and Maike Frye and MacLean, {Glenn A} and Kathleen H{\"u}bner and Bauer, {Daniel E} and Kanki, {John P} and Guillaume Vogin and Huston, {Nicholas C} and Minh Nguyen and Yuko Fujiwara and Paw, {Barry H} and Dietmar Vestweber and Zon, {Leonard I} and Orkin, {Stuart H} and Daley, {George Q} and Shah, {Dhvanit I}",
note = "{\textcopyright} 2015 by The American Society of Hematology.",
year = "2015",
month = dec,
day = "24",
doi = "10.1182/blood-2015-07-659276",
language = "English",
volume = "126",
pages = "2811--20",
journal = "BLOOD",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "26",

}

RIS

TY - JOUR

T1 - Hematopoietic stem cells develop in the absence of endothelial cadherin 5 expression

AU - Anderson, Heidi

AU - Patch, Taylor C

AU - Reddy, Pavankumar N G

AU - Hagedorn, Elliott J

AU - Kim, Peter G

AU - Soltis, Kathleen A

AU - Chen, Michael J

AU - Tamplin, Owen J

AU - Frye, Maike

AU - MacLean, Glenn A

AU - Hübner, Kathleen

AU - Bauer, Daniel E

AU - Kanki, John P

AU - Vogin, Guillaume

AU - Huston, Nicholas C

AU - Nguyen, Minh

AU - Fujiwara, Yuko

AU - Paw, Barry H

AU - Vestweber, Dietmar

AU - Zon, Leonard I

AU - Orkin, Stuart H

AU - Daley, George Q

AU - Shah, Dhvanit I

N1 - © 2015 by The American Society of Hematology.

PY - 2015/12/24

Y1 - 2015/12/24

N2 - Rare endothelial cells in the aorta-gonad-mesonephros (AGM) transition into hematopoietic stem cells (HSCs) during embryonic development. Lineage tracing experiments indicate that HSCs emerge from cadherin 5 (Cdh5; vascular endothelial-cadherin)(+) endothelial precursors, and isolated populations of Cdh5(+) cells from mouse embryos and embryonic stem cells can be differentiated into hematopoietic cells. Cdh5 has also been widely implicated as a marker of AGM-derived hemogenic endothelial cells. Because Cdh5(-/-) mice embryos die before the first HSCs emerge, it is unknown whether Cdh5 has a direct role in HSC emergence. Our previous genetic screen yielded malbec (mlb(bw306)), a zebrafish mutant for cdh5, with normal embryonic and definitive blood. Using time-lapse confocal imaging, parabiotic surgical pairing of zebrafish embryos, and blastula transplantation assays, we show that HSCs emerge, migrate, engraft, and differentiate in the absence of cdh5 expression. By tracing Cdh5(-/-)green fluorescent protein (GFP)(+/+) cells in chimeric mice, we demonstrated that Cdh5(-/-)GFP(+/+) HSCs emerging from embryonic day 10.5 and 11.5 (E10.5 and E11.5) AGM or derived from E13.5 fetal liver not only differentiate into hematopoietic colonies but also engraft and reconstitute multilineage adult blood. We also developed a conditional mouse Cdh5 knockout (Cdh5(flox/flox):Scl-Cre-ER(T)) and demonstrated that multipotent hematopoietic colonies form despite the absence of Cdh5. These data establish that Cdh5, a marker of hemogenic endothelium in the AGM, is dispensable for the transition of hemogenic endothelium to HSCs.

AB - Rare endothelial cells in the aorta-gonad-mesonephros (AGM) transition into hematopoietic stem cells (HSCs) during embryonic development. Lineage tracing experiments indicate that HSCs emerge from cadherin 5 (Cdh5; vascular endothelial-cadherin)(+) endothelial precursors, and isolated populations of Cdh5(+) cells from mouse embryos and embryonic stem cells can be differentiated into hematopoietic cells. Cdh5 has also been widely implicated as a marker of AGM-derived hemogenic endothelial cells. Because Cdh5(-/-) mice embryos die before the first HSCs emerge, it is unknown whether Cdh5 has a direct role in HSC emergence. Our previous genetic screen yielded malbec (mlb(bw306)), a zebrafish mutant for cdh5, with normal embryonic and definitive blood. Using time-lapse confocal imaging, parabiotic surgical pairing of zebrafish embryos, and blastula transplantation assays, we show that HSCs emerge, migrate, engraft, and differentiate in the absence of cdh5 expression. By tracing Cdh5(-/-)green fluorescent protein (GFP)(+/+) cells in chimeric mice, we demonstrated that Cdh5(-/-)GFP(+/+) HSCs emerging from embryonic day 10.5 and 11.5 (E10.5 and E11.5) AGM or derived from E13.5 fetal liver not only differentiate into hematopoietic colonies but also engraft and reconstitute multilineage adult blood. We also developed a conditional mouse Cdh5 knockout (Cdh5(flox/flox):Scl-Cre-ER(T)) and demonstrated that multipotent hematopoietic colonies form despite the absence of Cdh5. These data establish that Cdh5, a marker of hemogenic endothelium in the AGM, is dispensable for the transition of hemogenic endothelium to HSCs.

KW - Animals

KW - Antigens, CD

KW - Cadherins

KW - Cell Differentiation

KW - Cell Lineage

KW - Electroporation

KW - Embryo, Mammalian

KW - Embryo, Nonmammalian

KW - Flow Cytometry

KW - Hemangioblasts

KW - Hematopoiesis

KW - Hematopoietic Stem Cells

KW - Immunohistochemistry

KW - Mesonephros

KW - Mice

KW - Mice, Knockout

KW - Microscopy, Confocal

KW - Zebrafish

KW - Journal Article

U2 - 10.1182/blood-2015-07-659276

DO - 10.1182/blood-2015-07-659276

M3 - SCORING: Journal article

C2 - 26385351

VL - 126

SP - 2811

EP - 2820

JO - BLOOD

JF - BLOOD

SN - 0006-4971

IS - 26

ER -