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Helicobacter-induced expression of Bcl-X(L) in B lymphocytes in the mouse model

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Helicobacter-induced expression of Bcl-X(L) in B lymphocytes in the mouse model : a possible step in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma. / Morgner, A; Sutton, P; O'Rourke, J L; Enno, A; Dixon, M F; Lee, A.

In: INT J CANCER, Vol. 92, No. 5, 01.06.2001, p. 634-40.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Morgner, A, Sutton, P, O'Rourke, JL, Enno, A, Dixon, MF & Lee, A 2001, 'Helicobacter-induced expression of Bcl-X(L) in B lymphocytes in the mouse model: a possible step in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma', INT J CANCER, vol. 92, no. 5, pp. 634-40.

APA

Morgner, A., Sutton, P., O'Rourke, J. L., Enno, A., Dixon, M. F., & Lee, A. (2001). Helicobacter-induced expression of Bcl-X(L) in B lymphocytes in the mouse model: a possible step in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma. INT J CANCER, 92(5), 634-40.

Vancouver

Morgner A, Sutton P, O'Rourke JL, Enno A, Dixon MF, Lee A. Helicobacter-induced expression of Bcl-X(L) in B lymphocytes in the mouse model: a possible step in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma. INT J CANCER. 2001 Jun 1;92(5):634-40.

Bibtex

@article{b4da64e8d9534feaa22ff2910c5edab7,
title = "Helicobacter-induced expression of Bcl-X(L) in B lymphocytes in the mouse model: a possible step in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma",
abstract = "Primary gastric mucosa-associated lymphoid tissue (MALT) lymphoma may develop from chronic infection with Helicobacter sp. in the mouse model. The mechanisms of pathogenesis remain unclear. Regulation of B-cell proliferation and death are important features to investigate. Proteins encoded by bcl-2 family genes, e.g., Bcl-X(L), regulate apoptosis; and alterations in the expression of these genes can contribute to the development of cancer. Our aim was to determine the role of Bcl-X(L) in B lymphocytes in the development of gastric MALT lymphoma associated with Helicobacter infection using the BALB/c mouse model. We analyzed 37 animals with Helicobacter-associated MALT (n = 25), low-grade MALT lymphoma (n = 10) and high-grade lymphoma (n = 2), investigating the in vivo distribution of Bcl-X(L) in B cells/B-lymphoma cells using immunohistochemical analysis. In vitro cultivation of B cells/B-lymphoma cells was employed to perform RT-PCR analysis of Bcl-X(L) mRNA expression after cell stimulation with Helicobacter antigen. We found significant Bcl-X(L) protein expression in B lymphocytes within MALT and low-grade MALT lymphoma, whereas there was no and minimal expression, respectively, of Bcl-X(L) in the 2 high-grade MALT lymphoma cases. Expression of bcl-X(L) mRNA in B lymphocytes was up-regulated in vitro upon Helicobacter-antigen stimulation and associated with prolonged cell survival. These findings support the hypothesis that Bcl-X(L) plays a role in the pathogenesis of B-cell MALT lymphoma by providing cell-survival signals and by triggering the acquisition of MALT.",
keywords = "Animals, B-Lymphocytes, Cell Division, Female, Helicobacter Infections, Immunohistochemistry, Lymphoma, B-Cell, Marginal Zone, Mice, Mice, Inbred BALB C, Proto-Oncogene Proteins c-bcl-2, RNA, Messenger, Stomach Neoplasms, bcl-X Protein, Journal Article, Research Support, Non-U.S. Gov't",
author = "A Morgner and P Sutton and O'Rourke, {J L} and A Enno and Dixon, {M F} and A Lee",
note = "Copyright 2001 Wiley-Liss, Inc.",
year = "2001",
month = jun,
day = "1",
language = "English",
volume = "92",
pages = "634--40",
journal = "INT J CANCER",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "5",

}

RIS

TY - JOUR

T1 - Helicobacter-induced expression of Bcl-X(L) in B lymphocytes in the mouse model

T2 - a possible step in the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma

AU - Morgner, A

AU - Sutton, P

AU - O'Rourke, J L

AU - Enno, A

AU - Dixon, M F

AU - Lee, A

N1 - Copyright 2001 Wiley-Liss, Inc.

PY - 2001/6/1

Y1 - 2001/6/1

N2 - Primary gastric mucosa-associated lymphoid tissue (MALT) lymphoma may develop from chronic infection with Helicobacter sp. in the mouse model. The mechanisms of pathogenesis remain unclear. Regulation of B-cell proliferation and death are important features to investigate. Proteins encoded by bcl-2 family genes, e.g., Bcl-X(L), regulate apoptosis; and alterations in the expression of these genes can contribute to the development of cancer. Our aim was to determine the role of Bcl-X(L) in B lymphocytes in the development of gastric MALT lymphoma associated with Helicobacter infection using the BALB/c mouse model. We analyzed 37 animals with Helicobacter-associated MALT (n = 25), low-grade MALT lymphoma (n = 10) and high-grade lymphoma (n = 2), investigating the in vivo distribution of Bcl-X(L) in B cells/B-lymphoma cells using immunohistochemical analysis. In vitro cultivation of B cells/B-lymphoma cells was employed to perform RT-PCR analysis of Bcl-X(L) mRNA expression after cell stimulation with Helicobacter antigen. We found significant Bcl-X(L) protein expression in B lymphocytes within MALT and low-grade MALT lymphoma, whereas there was no and minimal expression, respectively, of Bcl-X(L) in the 2 high-grade MALT lymphoma cases. Expression of bcl-X(L) mRNA in B lymphocytes was up-regulated in vitro upon Helicobacter-antigen stimulation and associated with prolonged cell survival. These findings support the hypothesis that Bcl-X(L) plays a role in the pathogenesis of B-cell MALT lymphoma by providing cell-survival signals and by triggering the acquisition of MALT.

AB - Primary gastric mucosa-associated lymphoid tissue (MALT) lymphoma may develop from chronic infection with Helicobacter sp. in the mouse model. The mechanisms of pathogenesis remain unclear. Regulation of B-cell proliferation and death are important features to investigate. Proteins encoded by bcl-2 family genes, e.g., Bcl-X(L), regulate apoptosis; and alterations in the expression of these genes can contribute to the development of cancer. Our aim was to determine the role of Bcl-X(L) in B lymphocytes in the development of gastric MALT lymphoma associated with Helicobacter infection using the BALB/c mouse model. We analyzed 37 animals with Helicobacter-associated MALT (n = 25), low-grade MALT lymphoma (n = 10) and high-grade lymphoma (n = 2), investigating the in vivo distribution of Bcl-X(L) in B cells/B-lymphoma cells using immunohistochemical analysis. In vitro cultivation of B cells/B-lymphoma cells was employed to perform RT-PCR analysis of Bcl-X(L) mRNA expression after cell stimulation with Helicobacter antigen. We found significant Bcl-X(L) protein expression in B lymphocytes within MALT and low-grade MALT lymphoma, whereas there was no and minimal expression, respectively, of Bcl-X(L) in the 2 high-grade MALT lymphoma cases. Expression of bcl-X(L) mRNA in B lymphocytes was up-regulated in vitro upon Helicobacter-antigen stimulation and associated with prolonged cell survival. These findings support the hypothesis that Bcl-X(L) plays a role in the pathogenesis of B-cell MALT lymphoma by providing cell-survival signals and by triggering the acquisition of MALT.

KW - Animals

KW - B-Lymphocytes

KW - Cell Division

KW - Female

KW - Helicobacter Infections

KW - Immunohistochemistry

KW - Lymphoma, B-Cell, Marginal Zone

KW - Mice

KW - Mice, Inbred BALB C

KW - Proto-Oncogene Proteins c-bcl-2

KW - RNA, Messenger

KW - Stomach Neoplasms

KW - bcl-X Protein

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 11340565

VL - 92

SP - 634

EP - 640

JO - INT J CANCER

JF - INT J CANCER

SN - 0020-7136

IS - 5

ER -